2B82,1Z5G


Conserved Protein Domain Family
HAD_CBAP

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cd07499: HAD_CBAP 
Click on image for an interactive view with Cn3D
molecular class B acid phosphatases, similar to Escherichia coli AphA
class B acid phosphatases (CBAPs) have been detected in a minority of bacterial species which include a number of major pathogens such as Escherichia coli, Haemophilus influenzae, and Streptococcus pyogenes. This family includes the CBAP Escherichia coli AphA. The purified enzyme is a broad-spectrum nucleotidase highly active against both 3'- and 5'-mononucleotides and monodeoxynucleotides, which can also act as a phosphotransferase. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319802
View PSSM: cd07499
Aligned: 7 rows
Threshold Bit Score: 318.594
Threshold Setting Gi: 15675094
Created: 1-Apr-2008
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 20 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                          #####        #             ##     #                           
2B82_A        25 VAQIENSLAGrpPMAVGFDIDDTVLF-SSPGFWRGKKTFSPeseDYLKNPVFWEKMNn-gWDEFSIPKEVARQLIDMHVR 102
1Z5G_A        28 VAQIENSLTGrpPMAVGFDIDDTVLF-SSPGFWRGKKTYSPdsdDYLKNPAFWEKMNn-gWDEFSIPKEAARQLIDMHVR 105
gi 1176345    49 VDQIKQSLEGkaPINVSFDIDDTVMLfSSPCFYHGQQKFSPgkhDYLKNQDFWNEVNa-gCDKYSIPKQIAIDLINMHQA 127
gi 15675094   50 IADIASSLEGqkPITVSFDIDDTLLF-TSQYFQYGKEYITPgsfDFLHKQKFWDLVAk-rGDQDSIPKEYAKQLIAMHQK 127
gi 374110466  52 VADIQKELQGkaPFAVGFDIDDTTLF-STPVFYRGQQEFSPngySYLTNQDFWDKANc-gWDAFSMPKNIAKELIAMHQE 129
gi 374110461  54 IEQVAESLKGkaPISVGFDIDDTLLF-SSPAFFYGKQKFSPdsnAFLKNQKFWDAVSssgWDRFSIPKDSGRALMELHLK 132
gi 374110458  50 AESIQKELKGkpPITVSFDIDDTVLV-SSQCFYYGKQTFSPdslDYLHNQAYWDFVAd-gCDNYSIPKESAKKLIDMHQA 127
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                ###                                    #         #             ##  ##   
2B82_A       103 RGDAIFFVTGRSPTk------tETVSKTLADNFhipatnMNPVIFAGDkpg---qnTKSQWLQDKNiRIFYGDSDNDITA 173
1Z5G_A       106 RGDSIYFVTGRSQTk------tETVSKTLADNFhipaanMNPVIFAGDkpe---qnTKVQWLQEKNmRIFYGDSDNDITA 176
gi 1176345   128 RGDQVYFFTGRTAGk------vDGVTPILEKTFni--knMHPVEFMGSrert-tkyNKTPAIISHKvSIHYGDSDDDVLA 198
gi 15675094  128 RGDKIVFITGRTRGsmykkgeiDKTAKSLAKDFkl--dkPIAINYTGDkavkpyqyDKTYYIKKNGsQIHYGDSDEDINA 205
gi 374110466 130 RGDSIYFITGRTGSd------cNFTTEYLKKTFdi--kdMHDVIFAGSsrt---eyTKTKYIKNNDiKIYYGDADGDIIS 198
gi 374110461 133 RGDHVYFITGRPMPsn----gkEDLTQTLKDDFkipdnqLNKVIFAGTkq-----dAKVEYMQKYHiTIFYGDSDNDIQD 203
gi 374110458 128 RGDQVIFITGRTPHrlhkagqmDQLARILERAFhi--knMKPINYTHDkpiapykyDKTRFMVENRvSIHYGDSNDDILA 205
                        170       180       190
                 ....*....|....*....|....*....|....*...
Feature 1                         ##                   
2B82_A       174 ARDvGARGIRILRASNSTYKPLPQAGAFGEEVIVNSEY 211
1Z5G_A       177 ARDcGIRGIRILRAANSTYKPLPQAGAFGEEVIVNSEY 214
gi 1176345   199 AKEaGVRGIRLMRAANSTYQPMPTLGGYGEEVLINSSY 236
gi 15675094  206 AKEaGARPIRILRAPNSTNLPLPKAGGYGEEVLENSAY 243
gi 374110466 199 ARDaGAEGIRVMRAANSSYTPIPKNGIYGERVLKDSQY 236
gi 374110461 204 ARKaGAEGIRVLRPLNSTNKPMPKNGAFGEKVIVNSQY 241
gi 374110458 206 AREaGIRGIRVIRSATSTNRPLPLNGGYGEEVVEDSSY 243

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