2G07,2VKQ,2G09,4NV0


Conserved Protein Domain Family
HAD_5NT

?
cd07504: HAD_5NT 
Click on image for an interactive view with Cn3D
haloacid dehalogenase (HAD)-like 5'-nucleotidases similar to human cytosolic IIIA and IIIB
5'-nucleotidases dephosphorylate nucleoside 5prime-monophosphates. This family includes human 5'-nucleotidase, cytosolic IIIA (cN-IIIA, previously called cN-III; NT5C3A) the main pyrimidine 5'-nucleotidase in erythrocytes which dephosphorylates the pyrimidine nucleotides CMP, UMP, TMP, and the purine 7-methylguanosine monophosphate (m7GM), and possesses phosphotransferase activity. It also includes human 5'-nucleotidase, cytosolic IIIB (cN-IIIB; NT5C3B) which has a strong preference for m7GMP, dephosphorylates CMP and UMP and, with significantly lower efficiency, GMP and AMP, and can also act as a phosphotransferase. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
?
PSSM-Id: 319807
View PSSM: cd07504
Aligned: 16 rows
Threshold Bit Score: 388.98
Threshold Setting Gi: 240254604
Created: 20-Mar-2008
Updated: 18-Aug-2016
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                #####               #                     #   #  #      
2G07_A        25 NPTRVEEIICGLIKGGaaKLQIITXFDXTLSRFSYNGKRcp-tCHNIIDNckl-vtDECRRKLLQLKEQYYAIEVDPvlt 102
2VKQ_A        20 NPTRVEEIICGLIKGGaaKLQIITDFDMTLSRFSYKGKRcp-tCHNIIDNckl-vtDECRKKLLQLKEKYYAIEVDPvlt 97
gi 147901387  14 DPERVEELICRLIKGGasKLQVITDFDMTLSKFSHNGKRcp-tCHNIIDNskl-itDECRKKLFQLKEKYYAIEIDPdlt 91
gi 476007845  17 QPGRVQEIVGALRKGGgdRLQVISDFDMTLSRFAYNGKRcp-sSYNILDNski-isEECRKELTALLHHYYPIEIDPhrt 94
gi 123911034  15 DPEGLQDKITRIQRGGqeKLQIISDFDMTLSRFSRNGERcp-tCYNIIDNsni-isDEGRKKLKCLFDIYYPLEIDPkks 92
gi 392902088  20 DIKSLTDKLQNIVSGGadQLMVISDFDFTLSRFADKSGNrcssCYCVFDSavgtnnPEWCRKFVGLYHKYGPIEHDHsls 99
gi 7291759    31 DPAEVERIINEFVIGGpeRMQIVSDFDYTITKQRTEDGGavpsSFGIFNAcqs-lpENFKAETDKLYHKYRPIEIDPhmp 109
gi 74872158   31 DPAEVERIINEFVIGGpeRMQIVSDFDYTITKQRTEDGGavpsSFGIFNAcqs-lpENFKAETDKLYHKYRPIEIDPhmp 109
gi 22096397   95 DPTAVAAKLRKMVVGGagKTVVISDFDYTLSRFANEQGErlstTHGVFDDnvmrlkPELGQKFVDLKNKYYPIEFSPnlt 174
gi 240254604  14 HPRALTDKMTLIRDAGpsKFQVIADFDATLTRYRVNGLRg--rQTSHGLLqq--gdAYYDAKRQALYDHYHPLEISPvip 89
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  ##  #                                              ###                
2G07_A       103 veEKFPYXVEWYTKSHGLLieqgipkAKLKEIVADSDvxLKEGYENFFGKLQqhgIPVFIFSAGIGDVLEEVIRQagvy- 181
2VKQ_A        98 veEKYPYMVEWYTKSHGLLvqqalpkAKLKEIVAESDvmLKEGYENFFDKLQqhsIPVFIFSAGIGDVLEEVIRQagvy- 176
gi 147901387  92 mkEKYPYMVEWYMKSHTLLidqrlqkDKLAEAVRESDvkLKEGYEMFFDILHkqsIPVFIFSAGLGDILEEVIRQagvy- 170
gi 476007845  95 vkEKLPHMVEWWTKAHNLLcqqkiqkFQIAQVVRESNamLREGYKTFFNTLYhnnIPLFIFSAGIGDILEEIIRQmkvf- 173
gi 123911034  93 ieEKYPLMVEWWSKAHDLFyeqriqkDRLAQVVKESQatLRDGYDLFFNSLYqreIPLFIFSAGIGDVLEEIIRQagvf- 171
gi 392902088 100 ieEKVPFMEAWWQQSHGLIiqggfkkQAIDDYVAHCNiqLRDNADIMMKKMTnhaVPFIIFSAGIGTIIEMYLRHkfgrv 179
gi 7291759   110 iaEKVQYMIEWWTKSGELTsgfpfdqSEIDQIASKYThaLRDRTHEFFADLQrlgIPTLVFSAGLGNSVVSVLRQanvl- 188
gi 74872158  110 iaEKVQYMIEWWTKSGELTsgfpfdqSEIDQIASKYThaLRDRTHEFFADLQrlgIPTLVFSAGLGNSVVSVLRQanvl- 188
gi 22096397  175 meEKIPHMEKWWGTSHSLIvnekfskNTIEDFVRQSRivFKDGAEDFIEALDahnIPLVIFSAGIGNIIEYFLQQklgai 254
gi 240254604  90 idEKTKLMEEWWGKTHELLieggltyDAIKKSVANSSiaFREGVSELFEFLEkkeIPVLIFSAGLADVIEEVLRQnldrt 169
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                    #  #                                      ##  ##    
2G07_A       182 hsnVKVVSNFXDFDEnGVLKGFKGELIHVFNKHDGAL---------KNTDYFSql------kdNSNIILLGDSQGDLRXA 246
2VKQ_A       177 hpnVKVVSNFMDFDEtGVLKGFKGELIHVFNKHDGAL---------RNTEYFNql------kdNSNIILLGDSQGDLRMA 241
gi 147901387 171 hpdVKVISNFMEFDEnGIITGFKGELIHVYNKHDGAL---------KNTEYFGhl------kdNSNIILLGDSLGDLAMA 235
gi 476007845 174 hpnIHIVSNYMDFNEdGFLQGFKGQLIHTYNKNSSAC---------ENSGYFQql------egKTNVILLGDSIGDLTMA 238
gi 123911034 172 hpnTKVVSNYMDFDDnGILTGFKGDLIHTYNKNSSVL---------KDTEYFKei------shRTNILLLGDTLGDLTMA 236
gi 392902088 180 esnTHVVSNMMGFDDdGYVNSFSDPLIHVFCKNSSVMp--------ADRTFSEqi------egRKNVILLGDSVGDAFMD 245
gi 7291759   189 hpnVKVVSNFLQFRD-GLLDGFQQPMIHTFNKNETVL---------NETSEYYdlv-----htRDHIIVMGDSIGDADMA 253
gi 74872158  189 hpnVKVVSNFLQFRD-GLLDGFQQPMIHTFNKNETVL---------NETSEYYdlv-----htRDHIIVMGDSIGDADMA 253
gi 22096397  255 prnTHFISNMILFDEdDNACAFSEPLIHTFCKNSSVIq--------KETSFFHdi------agRVNVILLGDSMGDIHMD 320
gi 240254604 170 fknVKIVSNRMVFNDdGQLVSFKGKLIHVLNKNEHALdmaaplhdrLGVDIGEedeenvnmkeRRNVLLMGDHLGDLRMS 249
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*..
Feature 1                                                                 
2G07_A       247 DGvanveHILKIGYLNDrvd--elLEKYXDSYDIVLvke----eslEVVNSILQKTL 297
2VKQ_A       242 DGvanveHILKIGYLNDrvd--elLEKYMDSYDIVLvqd----eslEVANSILQKIL 292
gi 147901387 236 DGvpnveNVLKIGFLNDkve--elIEKYMDSYDIVLvkd----dtlDVANSIIQKIL 286
gi 476007845 239 DGvpgvqNILKIGFLNDkve--erRERYMDSYDIVLekd----etlDVVNGLLQHIL 289
gi 123911034 237 DGvstveNIIKIGFLNDkve--elTEQFLQSYDIVLlrd----etlDVVNGILQFVT 287
gi 392902088 246 VGveeeqVSLKIGFVNHdad--klVDKYLNYFDIVLvdd----qsmDIPNQILEAIY 296
gi 7291759   254 SGvpassHIMKIGFLFDhve--anMKKYMDTFDIVLvdd----qtmDVPRTLLSLIE 304
gi 74872158  254 SGvpassHIMKIGFLFDhve--anMKKYMDTFDIVLvdd----qtmDVPRTLLSLIE 304
gi 22096397  321 VGverdgPTLKVGYYNGslddtaaLQHYEEVYDIVLihd----ptlNVAQKIVDIIN 373
gi 240254604 250 DGld-yeTRISIGFLNDnie--ksLESYRESFDLVYlndapmwgalELVSRLFSTEA 303

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap