1XVI,3ZUP,1WZC,3ZX4


Conserved Protein Domain Family
HAD_Pase

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cd07507: HAD_Pase 
Click on image for an interactive view with Cn3D
haloacid dehalogenase-like superfamily phosphatase similar to Pyrococcus horikoshii mannosyl-3-phosphoglycerate phosphatase and Persephonella marina glucosyl-3-phosphoglycerate phosphatase
This family includes Pyrococcus horikoshii and Thermus thermophilus HB27 mannosyl-3-phosphoglycerate phosphatases (MpgPs) which catalyze the dephosphorylation of alpha-mannosyl-3-phosphoglycerate (MPG) to produce alpha-mannosylglycerate (MG), and Persephonella marina glucosyl-3-phosphoglycerate phosphatase (GpgP) which catalyzes the dephosphorylation of glucosyl-3-phosphoglycerate (GPG) to produce glucosylglycerate (GG). It also includes Methanococcoides burtonii MpgP protein which is able to dephosphorylate GPG to GG, and MPG to MG. Similar flexibilities in substrate specificity have been confirmed in vitro for the MpgPs from Thermus thermophiles and Pyrococcus horikoshii. Screens with natural substrates have not yet detected activity for another member Escherichia Coli YedP. Members of this family belong to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319810
View PSSM: cd07507
Aligned: 12 rows
Threshold Bit Score: 259.603
Threshold Setting Gi: 150421599
Created: 21-Mar-2008
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 24 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on Pyrococcus horikoshii and Thermus thermophiles mannosyl-3-phosphoglycerate phosphatase, and on other haloacid dehalogenase (HAD) superfamily members with structure
  • Structure:1WZC: Pyrococcus horikoshii mannosyl-3-phosphoglycerate phosphatase binds Mg2+ and phosphate ion in the active site
    View structure with Cn3D
  • Structure:3ZX4: Thermus thermophiles mannosyl-3-phosphoglycerate phosphatase binds 2-o-alpha-mannosyl-d-glycerate, Mg2+, and phosphate ion in the active site
    View structure with Cn3D
  • Structure:3ZUP: Thermus thermophiles mannosyl-3-phosphoglycerate phosphatase binds 2-o-alpha-mannosyl-d-glycerate, Mg2+, and phosphate ion in the active site
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #####                             ###   #                                   
1XVI_A        11 LVFSDLDGTLLDShsydwqpAAPWLTRLREanVPVILCSSKTSAEMLYLqktl--glqgLPLIAENGAVIQLAeqwqeid 88
3ZUP_A         2 IVFTDLDGTLLDErge-lgpAREALERLRAlgVPVVPVTAKTRKEVEALgl-------ePPFIVENGGGLYLPrdwpvra 73
1WZC_A         4 LIFLDIDKTLIPGyep--dpAKPIIEELKDmgFEIIFNSSKTRAEQEYYrkel---eveTPFISENGSAIFIPkgyfpfd 78
3ZX4_A         2 IVFTDLDGTLLDErge-lgpAREALERLRAlgVPVVPVTAKTRKEVEALgl-------ePPFIVENGGGLYLPrdwpvra 73
gi 161701642   5 VIFTDLDGTLLNHedysfkdAIPSLERIKKkgIPLVIVTSKTKKEVELIqkel---gieEPFIVENGAAVFFPkgyrgfn 81
gi 32365727   10 VIFTDLDGTLLDLhtydpgpAREAVARLQErgIPIVFCSSKTRAEQLSYrrql---girDPFIVENGATIFIPrgyftvp 86
gi 52550565    4 VLFTDLDGTMLDLydysydaALPALEALKTrkIPVVFCTAKTLVENEYYrkel---gidDPFIVENGGAIFVPenyfsfr 80
gi 91203792    4 IIITDLDGTLLDHttysfekAKEALALVRArnIPVVICTSKTQAEIEVYrekl---dnkDPFIPENGGAIIIPdgyfksv 80
gi 150421599   9 VIFTDLDNTLVGPggeageaGEVYLEALDLg-YRVVPVTSKSIYEIVELwdsigvppgeRIALAESGGAIYGPrgslarp 87
gi 121686883   4 IIFTDLDGTLIDHdtysydaARPALDLLKEkeIPLIFCTSKTRAELEVYvdel---echHPFISENGGAIFIPkdhfsie 80
gi 296103574  19 LVFSDLDGSLLDIhtyawqpAMPWLDKLQDfqVPLILCSSKTAAEMQDIqqdl--glegLPFIAENGAVIQPDvrwekaq 96
gi 33240179   11 WIVTDIDGTLMDHqyd-fspALSTIKWLQElgIPIIPCTSKTAAEVRALrlel---gitDPFIVENGGAIYGDelssnee 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               #           #         #  #    #          
1XVI_A        89 gf--------priisgisHGEISLVLNTLREkeHFKFTTFddvddatiaewTGLSRSQAALTQLHEASVTLiwrds---d 157
3ZUP_A        74 grpk---ggyrvvslawpYRKVRARLREAEAlaGRPILGYgdltaeavarlTGLSREAARRAKAREYDETLvlcp----- 145
1WZC_A        79 vkgke-vgnyivielgirVEKIREELKKLENiyGLKYYGNstkee--iekfTGMPPELVPLAMEREYSETIfewsr---- 151
3ZX4_A        74 grpk---ggyrvvslawpYRKVRARLREAEAlaGRPILGYgdltaeavarlTGLSREAARRAKAREYDETLvlcp----- 145
gi 161701642  82 ircdq-enryciiklgrdYREIRDFIEKIKDkfKIKGFGDmtvee--ivrlTDLPYDRAELAKERDFTEPFiie------ 152
gi 32365727   87 rearptshglevielgrpAAEIRRALREIRKetGLSFRGYhemtveevaelTGLPPEAARRAMTREYSETIivdfg---p 163
gi 52550565   81 feckk-rgdycvvefgalYGELRDALRAIKEetGFKITGFgdmtaeevaadANLSVELAKLAKQKEYNESFifd------ 153
gi 91203792   81 idkkn--gnyrivrlgtpYKEIVAALHKIREscGIAITGFsdltpgkisaiTGIDISSAIRAKQRLYSEPIfiesgheni 158
gi 150421599  88 tgfns-evgleytalgkpLASIDALLDSLAEtcGAVRLSKadate--aqliTGLPRERAALAARREYLEVIwsrsq---e 161
gi 121686883  81 lkdvheignykviefgtsYTRIRGVLEDIRKktGFKITGFgdldaegvskdTGLDIRSAKLAKLREYDEAFrleed---e 157
gi 296103574  97 -----------rltmgmlHRDIRPFIEQIRLqaGYKFTTFddvddrvigewTGLSRYRSTLARKHEASVTLiwrdt---d 162
gi 33240179   87 w----------elvlgksYKYLRSKLELISLelGYKLIPLndlsyqeinelTGLEGRGIELALDRKWSVPFlnpkds-dr 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                               #### #       #                         ##  ##            
1XVI_A       158 ermaQFTARLnelgl----qfmqGARFWHVldasaGKDQAANWIIATYqqlsg-krptTLGLGDGPNDAPLLEVmDYAVI 232
3ZUP_A       146 eeveAVLEALeavgl----ewthGGRFYHAak-gaDKGRAVARLRALWpdpe--earfAVGLGDSLNDLPLFRAvDLAVY 218
1WZC_A       152 dgweEVLVEGgfk-------vtmGSRFYTVhg-nsDKGKAAKILLDFYkrlg---qieSYAVGDSYNDFPMFEVvDKVFI 220
3ZX4_A       146 eeveAVLEALeavgl----ewthGGRFYHAak-gaDKGRAVARLRALWpdpe--earfAVGLGDSLNDLPLFRAvDLAVY 218
gi 161701642 153 --deKDIKDLeeiaekegfkitkGGRFYHLigkgqDKGRAVQIVKKVFeenyg-evplTVGLGDSRNDIPMLREvDIPIL 229
gi 32365727  164 adwqRFNAALvahgl----vcfaGGRFHTVvglgtDKGQAVHRLAELYrqqh--gailTVGLGDSPNDVPMLRAvDRPFL 237
gi 52550565  154 -epeSEAAVLfekikekgfaathGGRYYNIhgmnaDKGKAVRALTELFkrey--gevrTFGVGDSMNDISMLNAvEQPAV 230
gi 91203792  159 nkikKIFNALgfn-------fmeGGRFHHIfslntDKGKAVRMLINMYcdhyh-dqirTIGIGDSHIDLPMLAAvDVPVI 230
gi 150421599 162 cldtILSTALryre-----ltyvHRAPRTVqi-aaHRGKGMAIDAALQepllrpcakvVVTAGDSSHDIPIIERgMLAFR 235
gi 121686883 158 nataKVIELIhaagl----nytkGGRYWHImg-dnDKGKAVRALTEIYrqqf--tevvTIALGDSLNDLPMLKAvDIPFL 230
gi 296103574 163 ekmvQFEEELaqrgl----kclqGARFWHIldarcGKDVAVNWLVAQYreqeg-imptTLGLGDGPNDAPLLDSvDFAVV 237
gi 33240179  156 eritEIASSLcts-------iykGNRMSHLlgegsHKGKAVIELKKFLnqp----nikVVALGDSQNDLPLLEVaDIAIV 224
                        250       260       270
                 ....*....|....*....|....*....|..
Feature 1                                        
1XVI_A       233 Vkglnregvhlhd-edparvwrtqreGPEGWR 263
3ZUP_A       219 Vgrgdppeg----------vlatpapGPEGFR 240
1WZC_A       221 Vgslkhkkaqn-----vssiidvlevIKHHHH 247
3ZX4_A       219 Vgrgdppeg----------vlatpapGPEGFR 240
gi 161701642 230 Iphinkkyesvn----lpgiikaeypGSKGWN 257
gi 32365727  238 Vqrpdgtwesvd----vpglvpipapGPEGWC 265
gi 52550565  231 Vknkkgawldis----lpglykakgeGPEGWA 258
gi 91203792  231 Vrkktgdydkri---qlpnlvyadgiGPAGWN 259
gi 150421599 236 Vdynrdwsrlv-----kpiymvipyeAPKAWT 262
gi 121686883 231 Vqkpdgkydpsi---ilteikhaegiGPVGWN 259
gi 296103574 238 Ikginrqgitlrd-dnparvyhtqqaGPAGWH 268
gi 33240179  225 IpsrngpnkflkkgidkgefllapapHSEGWA 256

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