2HO4,3HLT,2X4D


Conserved Protein Domain Family
HAD_PPase

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cd07509: HAD_PPase 
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inorganic pyrophosphatase similar to a human phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP)
LHPP hydrolyzes nitrogen-phosphorus bonds in phospholysine, phosphohistidine and imidodiphosphate as well as oxygen-phosphorus bonds in inorganic pyrophosphate in vitro. This family also includes human haloacid dehalogenase like hydrolase domain containing 2 protine (HDHD2) a phosphatase which may be involved in polygenic hypertension. Members of this family belong to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319812
View PSSM: cd07509
Aligned: 18 rows
Threshold Bit Score: 319.225
Threshold Setting Gi: 31792418
Created: 11-Jun-2008
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on human LHPP, on human and mouse HDHD2, and on other haloacid dehalogenase (HAD) superfamily members with structure
  • Structure:2X4D: human LHPP binds phosphoric acid and Mg2+ in the active site
    View structure with Cn3D
  • Structure:3HLT: human haloacid dehalogenase-like hydrolase domain containing 2 (HDHD2), binds Na+ and sulfate ion in the active site
    View structure with Cn3D
  • Structure:2HO4: Mus musculus haloacid dehalogenase-like hydrolase domain containing 2 protein binds Mg2+ and phosphate ion in the active site
    View structure with Cn3D
  • Structure:2X4D
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #####                                 ######                               
2HO4_A         8 KAVLVDLNGTLHIEda-----aVPGAQEALKRLratSVXVRFVTNTTKEtkKDLLERLKKLEFeisEDEIFTSLtAARNL 82
2X4D_A        13 RGVLLDISGVLYDSgagg-gtaIAGSVEAVARLkrsRLKVRFCTNESAAsrAELVGQLQRLGFdisEQEVTAPApAACQI 91
gi 66045799    4 DSLLFDIDGTLLLKgq-----pLPGAADALSFArsqGLRLQLLTNTTAKmpEELAEELCRAGIevvPDEIQTATtSCLGY 78
gi 74132209   34 KALLLDIDGTMVFKgh-----aIPGAAEAIEAArmaGLQLRFLTNITGQlpSTIAEDLQRHGInvrAEEIHTAStSCARY 108
gi 148235235   9 RAVLLDVSGVLYDSggagggsaIQGSVDAVNRIrhaGLKLRFCTNESQAtrSHFAQKLKRFGFsisEEEVTAPGpAATRL 88
gi 158705883  12 RGVLLDISGVLYDSgagg-gtaIAGSVEAVARLkrsRLKVRFCTNESQKsrAELVGQLQRLGFdisEQEVTAPApAACQI 90
gi 148922898  14 KGVILDMCGVLYDSgegg-graIHGSVEAVKRLmdsGLMLRFCTNETQNtrERFVQKLRVMGFdisVSHVFSPApAVVQI 92
gi 19920940    4 KGALIDLSGTLHVEde-----pTPNAVEALKRLrdsGVLVKFVTNTTKDskATLHERLCRIGFqldASEIYSSLsAAVSY 78
gi 17557870    8 NGFLLDITGVLYNSiyksdgvaVPKSAEAVNFLy-qHSKVKFLSNAKGNsnRNVARRLQRLGInvrEEDVITPApVVAQY 86
gi 31792418    9 AAVLFDIDGVLVLSwr-----aIPGAAETVRQLthrGIACAYLTNTTTRtrRQIAEALGAAGIpvaADDVITAGvLTAEY 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                     #  
2HO4_A        83 IEqkq--vrPXLLLddralpef------------tgvqtqdPNAVVIGlapehFHYQLLNQAFRLLl--dGAPLIAIHKA 146
2X4D_A        92 LKerg--lrPYLLIhdgvrsef------------dqidtsnPNCVVIAdagesFSYQNMNNAFQVLmeleKPVLISLGKG 157
gi 66045799   79 LQqhp-hlkCHLLVpdsirpvfr----------giatddtnPDVVVISdigeaFDYATLNRCFRMLr--gGARLIALQKN 145
gi 74132209  109 LKslg-dvsCFFLMpesvnsmfe----------giardhvsPDVVVIGdigesFDYACLNQAFGYLh--kGARLVVPHKN 175
gi 148235235  89 MKerg--lrPHLLVhndllpef------------esveksdPNCVLIGdaaenFSYKNVNRAFQVLinlqKPVLISLGKG 154
gi 158705883  91 LKeqg--lrPYLLIhdgvrsef------------dqidtsnPNCVVIAdagesFSYQNMNNAFQVLmeleKPVLISLGKG 156
gi 148922898  93 LQkrh--lrPHLLVhddlipef------------dgvdtssPNCVVIGdaaekFSYQNLNEAFRVLigleKPVLFSLGRG 158
gi 19920940   79 VEner--lnPYYILsedarqdf-----------ppedtrryKDSVVIGlapkaFNYEQLNEAFNVLlenkNHKLIAVHQG 145
gi 17557870   87 CRenk--lrPHLFVrddvleyf------------dgidtssPNCVVMGeveegFSFDRINRAFRILidmpKPLLITMGNG 152
gi 31792418   84 LHgaypgarCFLVNngditedlpgidvvlsteigpedcpeaPDVVVLGsagpqFDHRTLSRVYGWMl--dGVPVVAMHRN 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                        #                       ###  ##                 
2HO4_A       147 RYYkrkDGLALGPGPFVTALEYAtdtkaxVVGKPEKTFFLEALrdadcapeEAVXIGDDcRDDVDGAQNIGXLGILVKTG 226
2X4D_A       158 RYYaatSGLMLDVGPYMKALEYAcgikaeVVGKPSPEFFKSALqaigveahQAVMIGDDiVGDVGGAQRCGMRALQVRTG 237
gi 66045799  146 LFWfdrDGERLDCGAFIVGLEAAaqvqalVMGKPSPMFFEAALrkldtsasRTLVVGDDvLTDCAGAKAVGASSLLVRTG 225
gi 74132209  176 LFWfdpRGVRLDCGAFILGLEAAsgkqalVTGKPSPVFFTSVMdslgvkpsQTMIIGDDlLTDIAAAQHLEVAHALVLTG 255
gi 148235235 155 RYYketDGLKLDVGAYMKALEYAcdikaeVVGKPSPNFFLSALeemgakpeEALMIGDDiVHDIGGAKSCGLRAVLVRTG 234
gi 158705883 157 RYYketSGLMLDVGPYMKALEYAcgikaeVVGKPSPEFFKSALqaigveahQAVMIGDDiVGDVGGAQRCGMRALQVRTG 236
gi 148922898 159 RYYketDGLKLDVGVYMKALEYAcdvqaeVVGKPSSEFFKTVLndmnlqphEVVMVGDDlVNDVGGAQSCGMKGLQVRTG 238
gi 19920940  146 KYYkraEGLALGPGCFVKGLEFAtgrtakVIGKPNPYFFEGALagr--dpaSCVMIGDDaNDDIVGAMSMGMQGILVKTG 223
gi 17557870  153 KFFqrvDGPCIDVGAFAAALKFStncevlNIGKPSRFYFEQGMnalgmkpeEIVMVGDDlMSDVGGAQACGMRGVQVRTG 232
gi 31792418  162 MTWnttDGLRIDTGMYLTGMEQAcgktatAIGKPAAEGFLAAAdrvgvdpqQMVMIGDDlHNDVLAAQAVGMTGVLVRTG 241
                        250       260       270
                 ....*....|....*....|....*....|....*...
Feature 1                                              
2HO4_A       227 KYKaadeeki---------npppyltCESFPHAVDHIL 255
2X4D_A       238 KFRpsdehhp---------evkadgyVDNLAEAVDLLL 266
gi 66045799  226 KYDprlfeahrqnvdaviegiaefprWWMSGSAAGHLR 263
gi 74132209  256 KGAsytesd-----------vprperLWPSIAELSRFL 282
gi 148235235 235 KYRpsdekhp---------evtadgyVNNLAHAVDILL 263
gi 158705883 237 KFRpsdehhp---------evkadgyVDNLAEAVDLLL 265
gi 148922898 239 KYRpsdecdp---------svradayVDDLSAAVDAIL 267
gi 19920940  224 KYLpdvkps-----------ppptalLENFAEAVDWII 250
gi 17557870  233 KWRpdfekmp----------vtpdltADCLYDAVKLIA 260
gi 31792418  242 KFRqqtl---------------drwlAGASATRPHHVI 264

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