1RKQ,3MPO,3NIW,1NF2,3DNP


Conserved Protein Domain Family
HAD_Pase

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cd07516: HAD_Pase 
Click on image for an interactive view with Cn3D
phosphatase, similar to Escherichia coli Cof and Thermotoga maritima TM0651; belongs to the haloacid dehalogenase-like superfamily
Escherichia coli Cof is involved in the hydrolysis of HMP-PP (4-amino-2-methyl-5-hydroxymethylpyrimidine pyrophosphate, an intermediate in thiamin biosynthesis), Cof also has phosphatase activity against the coenzymes pyridoxal phosphate (PLP) and FMN. Thermotoga maritima TM0651 acts as a phosphatase with a phosphorylated carbohydrate molecule as a possible substrate. Escherichia coli YbhA is also a member of this family and catalyzes the dephosphorylation of PLP, YbhA can also hydrolyze erythrose-4-phosphate and fructose-1,6-bis-phosphate. Members of this family belong to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319818
View PSSM: cd07516
Aligned: 86 rows
Threshold Bit Score: 181.254
Threshold Setting Gi: 50365320
Created: 12-Mar-2008
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #####                             ##                                        
1RKQ_A         7 LIAIDMDGTLLlpdhtisPAVKNAIAAArarGVNVVLTTGRpYAGVHNYLkelhmeqpgDYCITYNGALVQkaa--dgST 84
gi 29375041    5 AIVMDIDGTLLtsekkisPKTRQALVAAqkqGLSLILASGRpTNGMRPLAdelemahynGHLLSYNGACVThhg--sqQQ 82
3NIW_A         7 LIVLDLDGTLTnskkeisSRNRETLIRIqeqGIRLVLASGRpTYGIVPLAnelrxnefgGFILSYNGGEIInwe--skEX 84
gi 15004774    6 VIIMDIDGTLTtskkiisEKTKKALKKAqelGAVLILASGRpTSGLMDFAkelemdknhGLLVSFNGARVVdce--tnKI 83
gi 16804736    5 AIILDIDGTLLnddkqisPETKKALITAqenGVKLILASGRpTTGMHVYAeqlemekhhGLLVSYNGAKVVdca--tnEE 82
gi 88195053    6 VVVLDMDDTLLnsdnvisEETANYLTAIqdeGYYVVLASGRpTEGMIPTArdlklpehhSYIISYNGSKTInmt--neEV 83
gi 1176309     5 AVFSDFNGTLLtsqhtisPRTVVVIKRLtanGIPFVPISARsPLGILPYWkql---etnNVLVAFSGALILnq---nlEP 78
1NF2_A         4 VFVFDLDGTLLndnleisEKDRRNIEKLs-rKCYVVFASGRmLVSTLNVEkkyf--krtFPTIAYNGAIVYlp---eeGV 77
gi 380033778   4 LIVSDIDGTLVdsqgqllPATITGVQAAvarGAQMVLASARsPKGMFGIAqql---ginSTMIAYNGALTAttdvndrLT 80
gi 22538214    4 LIFSDIDGTLInsnfkvtPKTRQGIKQIvaqGATFVPISARmPEAITPIMeqi---gidSYIISYNGALIQdm---qqKT 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1RKQ_A        85 VAQTal----syDDYRFLEKLSrev---------gsHFHALDr-------------------tTLYTAnrdisyytvhes 132
gi 29375041   83 LFNQtiskslsqQILEHLKQFDvipmi-----ndetFMYVNDvfhntlhl------etgdfniIEYESrggnfqlcewr- 150
3NIW_A        85 XYENvl----pnEVVPVLYECArtn---------hlSILTYDg-------------------aEIVTEnsldpyvqkeaf 132
gi 15004774   84 LFNEtmsidqgqAVLEHMKKFEvkpmi-----dkgdYMYVNDvfdneiqy------kgepfniIQYESrnnkfklcekh- 151
gi 16804736   83 LFNQaltveegkAVLEHMKQFEvkvmi-----dkedYMYTNDvydcyvpy------rgeeiniIQYESrggnfklcekd- 150
gi 88195053   84 EVSKsi----gkQDFDEIVDYCrdrgffvltyhdgqIIYDSEheymnieaeltglpmkrvddiKAYIQgd---------- 149
gi 1176309    79 IYSVqi----epKDILEINTVLaehp------llgvNYYTNNdchard----------venkwVIYERsvtkieihpf-- 136
1NF2_A        78 ILNEki----ppEVAKDIIEYIkpl---------nvHWQAYId-------------------dVLYSEkdneeiksyarh 125
gi 380033778  81 VFEEqavdpklaQAVQTVVAHDwpa--------asiSIYSNNnwlvatngp----weqqeatgIGYAPhvtdlgtwl--- 145
gi 22538214   78 IASHtm----dgQVALQVCSYVskhys-----kiawNVYRYH---------------------EWYSCdkenewvqkeee 127
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                          #             
1RKQ_A       133 fvatiplvfceaekmdpntqflKVMMIdepaildqaiaripqevkekyTVLKsapyFLEILdkrvnKGTGVKSLADVLgi 212
gi 29375041  151 -----------dlaarlnfplnKILIAgepaylqkyheaiyapfketvTAAFsapfYFEFTaknidKARSLEKLTLQLgi 219
3NIW_A       133 lnkxa-iretndfltditlpvaKCLIVgdagklipveselcirlqgkiNVFRsepyFLELVpqgidKALSLSVLLENIgx 211
gi 15004774  152 -----------dlaafadyplnKILTAgepeylkkhykemmepfkeslNCMFtadfYFEFTaegidKAKALDTVLIPMgy 220
gi 16804736  151 -----------dlaafldyrlsKILTAgdpaymqknyqammapfkdtlNCVFtadfYFEFTaqgidKAKALDTVLTPMgi 219
gi 88195053  150 --------------------vpKVMGVdyvanitearidlngvfndnvDATTskpfFLEFMakdvsKGNAIKALCHKLgy 209
gi 1176309   137 -------------devatrsphKIQIIgeaeeiieie-vllkekfphlSICRshanFLEVMhksatKGSAVRFLEDYFgv 202
1NF2_A       126 snvdy-rvepnlselvskmgttKLLLIdtperldelkeilserfkdvvKVFKsfptYLEIVpknvdKGKALRFLRERMnw 204
gi 380033778 146 --------------qrmptpvhKMMIMadpdiiqaieqrlsrnefsglAVYRsketYLEIVaagvtKAAALMKLLQDDqv 211
gi 22538214  128 ivglq-skemslmelekqdrihKLLLMgepslmgele-ntlkaqyphlSIAQsapyFIEIMapgieKGKSAKTLADYLdi 205
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                ### ##                                          
1RKQ_A       213 kpEEIMAIGDQENDIAMIEYAGVGVAVdNAIPSVKEVANfvtksNLEDGVAFAIEK 268
gi 29375041  220 aaEEVIAFGDGHNDYTMLEWAGTGIAMeNAVDELKSIATevtlsNDNDGIAVALTN 275
3NIW_A       212 trEEVIAIGDGYNDLSXIKFAGXGVAXgNAQEPVKKAADyitltNDEDGVAEAIER 267
gi 15004774  221 kkEEMIAFGDGHNDISMVKYAGIGVAMdNAVQELKNAADevtlsNEEDGIAYTLSK 276
gi 16804736  220 haENVIAFGDGHNDITMVEYAGTGIAMqNAVPELKAAASsvtlsNNEDGIAHVLNS 275
gi 88195053  210 svDQVIAFGDSMNDKSMFEVAGLAIAMgNASDELKQYANevtldHNENGIPHALKK 265
gi 1176309   203 qtNEVIAFGDNFNDLDMLEHVGLGVAMgNAPNEIKQAANvvtatNNEDGLALILEE 258
1NF2_A       205 kkEEIVVFGDNENDLFMFEEAGLRVAMeNAIEKVKEASDivtltNNDSGVSYVLER 260
gi 380033778 212 tpAETLAFGDNFNDVAMLQTAGIGVAMaNAPQPVKEAANivttdNNHNGIQLTLAQ 267
gi 22538214  206 slADSIAFGDNYNDLNLLEIVGKGFVMgNAPKDLQERIGnvtqdNDNDGIYYALVE 261

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