1RLO,1RLM,3L7Y,1RLT,2HF2,3DAO,2B30


Conserved Protein Domain Family
HAD_YbiV-Like

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cd07518: HAD_YbiV-Like 
Click on image for an interactive view with Cn3D
Escherichia coli YbiV sugar phosphatase/phosphotransferase and related proteins; belongs to the haloacid dehalogenase-like superfamily
Escherichia coli YbiV can act as both a sugar phosphatase and as a phosphotransferase. Members of this family belong to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319820
View PSSM: cd07518
Aligned: 29 rows
Threshold Bit Score: 160.827
Threshold Setting Gi: 78101559
Created: 12-Mar-2008
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on Escherichia coli sugar phosphatase YbiV, and on other haloacid dehalogenase (HAD) superfamily members with structure
  • Structure:1RLM: Escherichia coli sugar phosphatase YbiV, binds glycerol and Mg2+ in the active site
    View structure with Cn3D
  • Structure:1RLT: Escherichia coli sugar phosphatase binds aluminum fluoride and Mg2+ in the active site
    View structure with Cn3D
  • Structure:3DAO: Eubacterium rectale putative phosphatase (Eubrec_1417) binds PEG400 in the active site
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #####                               ###                     #              
1RLO_A         4 KVIVTXMDGTFLndaktynqprFMAQYQELKKrg-iKFVVASGNQYYQLISFFpel-kdeISFVAENGALVYehgkqlfh 81
1RLM_A         4 KVIVTDMDGTFLndakt-ynqpRFMAQYQELKkrgiKFVVASGNQYYQLISFFpel-kdeISFVAENGALVYehgkqlfh 81
gi 59714330    7 KFIAADMDGTLLnehse-ldpsFFDLYQQLEDkn-iIFAAASGRQYYSLLETFepv-kdrMMFIAENGTLVMypgeelys 83
gi 153937441   3 KFIATDLDGTLVnsegk-iynkVFNLINDLHKng-vKFAAASGRFYSQLNENFnsv-kedMILIAHNGALIKyskngqtl 79
gi 23308812    4 RLVATDMDGTLLnthhe-vpekFWDILEQMRAkg-iAFAPASGRQLATLQKQFgha-gepISYIAENGTVVVhdgeiisl 80
gi 380032667   4 AIIATDLDGTFLrddhqfdhhrFQAQLDQMNAqg-qHFVVASGNQLQHCIDVFdgi-ngeLTYVAENGGLVIdnhgnvla 81
gi 253755371   4 KIYASDMDGTFLredhsfdkerFRRILNQFKEkg-yLFVAASGRSMQSLKLVFedf-vdeIGFVAENGSIVEyqgqtifm 81
gi 253754864   4 RLIATDMDGTFLdgqgnfdrerFSRVLDQLDQkk-iPFVIASGNGIGRLLQLCqgf-edrLIFVADNGAHVYqngktvir 81
gi 15893812    3 KLIATDMDGTLLrsnge-fpneFPLILDSLLNkn-iMFSVASGRQYFTLKDNMagf-edkITFIAENGAFIVkdgkelfa 79
gi 380031804   4 NLIATDLNGTLLhqdqrfdkerFKRVLAQLRAin-vSLVLSSGNQYAHLRQLFkdvmadnLIVVAENGASIYaqdrlifd 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                     # #
1RLO_A        82 geltrhesrivigellkdkqlnfvacglq-----------sayvsenapeafvalmakhyhrlkpvkdyqeiddvlFKFS 150
1RLM_A        82 geltrhesrivigellkdkqlnfvacglq-----------sayvsenapeafvalmakhyhrlkpvkdyqeiddvlFKFS 150
gi 59714330   84 csmdtpsviniiktaraiegthivlcgkrsayi--etqdpkalaefskyyqrceyvedllavddefikvaichfdgTEEF 161
gi 153937441  80 yanyidkeyiksveklkrnfgeelilage-----------neafvvnpsqsikeefnfynvpyieyksfdevdkpvQKIS 148
gi 23308812   81 ttidsdtvhsiidavrasdidmgvvvcrp----------erayverndeafraeglkyyvsieevqdlheavnnevIKVA 150
gi 380032667  82 esliepalyqelliyvatepalagaeisv----------sgkqgayirpqddspimryylsrlqvvpslsaiddhiYKAT 151
gi 253755371  82 ddpispeiylpiiagidagpfgssrsmvlsgle---nfyllknaepqfleamtnyyahfrlvdtfeevreeiikinAKFS 158
gi 253754864  82 raiqqveveailhffkgrwadvclmlsndgniymqagagmpfagtdlpielaqmaafqnrvtyldnlsaypvsesiHKVG 161
gi 15893812   80 ktldrdmlskvvddakkvpdcklvlcgk------------ksayvldkssafieevekyyhksavvnsldeiddefFKIA 147
gi 380031804  83 gslsadqqrkfvtvdrhqplfkdayvilvg--------shgsytelgapdklvamarqfydhlhlvadlatvddtvKKIS 154
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                         #       #                     ### ##           
1RLO_A       151 LNlpdeqiplvidklhvaldgimkpvtsgfgfIDLIipglhKANGISRLLKrwdlspqnVVAIGDSGNDAEMLKMarYSF 230
1RLM_A       151 LNlpdeqiplvidklhvaldgimkpvtsgfgfIDLIipglhKANGISRLLKrwdlspqnVVAIGDSGNDAEMLKMarYSF 230
gi 59714330  162 VFpsfdanfges----------hqvvvsakiwLDVMnvvasKGAAIKHLQNtldfsfeeTMSFGDYLNDLEMLQEsyYSY 231
gi 153937441 149 YYvkdgikasmidylkenlnknlqfvasgdkwIDMMnkevsKGHAIKILQKkfniekdnTMVFGDYYNDITMFKQayYSY 228
gi 23308812  151 IFtfqdaekdcapiira-aspnanvvvsgqhwVDVMdpsanKGQALAALRDalgleesqTLVFGDYLNDTELIKAagKSY 229
gi 380032667 152 FSwqatdadahaalinqqfagrlratvsggngLDVIpphvnKATGLAYLQQhwhvapsqTAAFGDNGNDLEMLREadYSF 231
gi 253755371 159 PEemddarrwlndtf-----egvtamttgfdnIDIIpngsnKSVGLSHLCAhfgitrqdVVAFGDNQNDLDMFDFagLAL 233
gi 253754864 162 LWvpearvesiteafnqgfhgqlvavtsgygsVDILpqgihKAWGLEQVLTgldiepeqVMAFGDSDNDIELLSYvgYSY 241
gi 15893812  148 ICdykgsannsnvllspkwgklfqltvsgdiwLDIGrndvnKGTAIKFLQDkfnineneTMAFGDYYNDVSMLQSvyHSY 227
gi 380031804 155 IStspdqaadlvarandyfdgelrahdsgygvVDLVdqrvgKLPAIQFLATklgltaanVMAFGDGANDVPLLRYaaHSY 234
                        250       260       270
                 ....*....|....*....|....*....|..
Feature 1                                        
1RLO_A       231 AMGNAaeNIKQIAr-YATDDN-NHEGALNVIQ 260
1RLM_A       231 AMGNAaeNIKQIAr-YATDDN-NHEGALNVIQ 260
gi 59714330  232 AMENAhqKVKETAr-FMAPSN-KDAGVFTVIK 261
gi 153937441 229 AMENApeDVKEKA--NFIAGNnNENAVYKTIS 258
gi 23308812  230 AMSNAhpDILELA--DEIAPSnIEEGVIVVLE 259
gi 380032667 232 AMQNAiaPVKEMAtyLTSHDN-NHDGVLATID 262
gi 253755371 234 ATENAreEVKAQAd-WMIGHC-NDGAVLAYLE 263
gi 253754864 242 AMENAtdKVKAVAk-YMAPSH-LEAGVLQVIE 271
gi 15893812  228 VMENApdGVKKHGr-FIAKSN-DESGVIQVIK 257
gi 380031804 235 AMCNApaDIQAAAkhVTALDN-EHDGVLATIE 265

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