2FEA,2FEA


Conserved Protein Domain Family
HAD_Pase

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cd07524: HAD_Pase 
Click on image for an interactive view with Cn3D
phosphatase, similar to Bacillus subtilis MtnX; belongs to the haloacid dehalogenase-like superfamily
Bacillus subtilis recycles two toxic byproducts of polyamine metabolism, methylthioadenosine and methylthioribose, into methionine by a salvage pathway. The sixth reaction in this pathway is catalyzed by B. subtilis MtnX: the dephosphorylation of 2- hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HKMTP- 1-P) into 1,2-dihydroxy-3-keto-5-methylthiopentene. The hydrolysis of HK-MTP-1-P is a two-step mechanism involving the formation of a transiently phosphorylated aspartyl intermediate. Members of this family belong to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319826
View PSSM: cd07524
Aligned: 12 rows
Threshold Bit Score: 258.802
Threshold Setting Gi: 159029056
Created: 5-Jun-2008
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #####    # ##                                   #                           
2FEA_B         8 FIICDFDGTITxnDNIINIXKTFApp----eWXALKDGVLsktlsIKEGVGRXFGLlpsslKEEITSFVLEDakIREGFR 83
2FEA_A         8 FIICDFDGTITxnDNIINIXKTFApp----eWXALKDGVLsktlsIKEGVGRXFGLlpsslKEEITSFVLEDakIREGFR 83
gi 398365467   5 VIFTDFDGTVTleDSNDYLTDTLGfgk--ekRLKVFEGVLddtksFRQGFMEMLESih-tpFPECIKILEKKirLDPGFK 81
gi 24459281    8 IVFTDFDGTITlkDSNDYLTDNIGygp--ekRREGNIDILenrvkFRDAFKEMLDSvk-kpFPECIEILRKNikLDPGFK 84
gi 159029056   4 IVFCDFDGTITavETFAGMLKEFApdlsaqiMPQMYARTLs----LRRGVRQLLESipsqkYADIIAYAENKp-IRPGLA 78
gi 171988989   4 RILCDFDGTVTehDNIIALMTEFApp---eaFEPLKKGVLdqslsIQSGVGQMFRLlpsdrKQDYIDFLEQRavIRPGFK 80
gi 254764008   6 VLFCDFDGTITekDNIVAIVRKFApp----eWEALTEQILsqkisVQEGVGKLFQLlpsslRQDIIDFIVHEatIRPGFA 81
gi 259509732   4 HVICDFDGTITeeDNIIALMRRFApp----eWVELKDSVLnqtlsIREGVGQMFSLlpsnqQERYREFLQSTitLRAGFV 79
gi 218527838   4 IIFCDFDGTITetDNIFSLMTEFVpqesekiAKAMMEQTIs----FKDGLSAMFHLlsteqKDEVIQYLMDTavIREGFE 79
gi 74625279    8 YVFSDFDGTITlqDSNDYLTDNFGmgn--anRVNLNQQVLdgsisFRDAFAKMLDSvh-lsYDEALEVLKKNvaIDPSFK 84
gi 81558025    6 VLFCDFDGTITenDNIIAIMKQFApp----eWEALKDDILaerisVQEGVGKMFSLlpsslKGEIIDFLRRTtrLRAGFR 81
gi 81686407    4 QVFCDFDGTITnnDNIMSIMEKFApp----eAEEVKNRILsqelsIQEGVSQLFQLiptnlHDEIIQFLIETaeIRNGFH 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                        ##                                                              
2FEA_B        84 EFVAFINehEIPFYVISGGXDFFVYPLLegi-----vekDRIYCNHASFdn---------dYIHIDWphsckg----tcs 145
2FEA_A        84 EFVAFINehEIPFYVISGGXDFFVYPLLegi-----vekDRIYCNHASFdn---------dYIHIDWphsckg----tcs 145
gi 398365467  82 DTFEWAQenDVPVIVVSSGMKPIIKVLLtrlvgqesihkIDIVSNEVEIda--------hdQWKIIYkde---------- 143
gi 24459281   85 DFYDWCLseKIPVIVLSSGMEPIIRALLtdlvg-phadqIKIVSNQPKFldpegnewtveyHDDSDF------------- 150
gi 159029056  79 EFLAFLQeqSIPFIIISGGIQGMIETVLkregl--ldkvTAIYGVNLHTqge-------ylQVHSDWene---------- 139
gi 171988989  81 TLLAFAKsnGIDFAIVSGGIDFFVQPILqel-----ltdEAIYCNGSDFsg---------eTIRIEWphtcda----acn 142
gi 254764008  82 EFVSYCReeGIELLITSGGIDFFLEPILapfd----ladVPIYCNGSDFsg---------eRITITWpnacde----hct 144
gi 259509732  80 EFLQETQshGFRFDVVSGGMDFFVHPILegh-----vapEHIFCNHVDFsget-----arvTWPHACdvhc--------l 141
gi 218527838  80 DFVRYAQnhDIPFYIVSGGVDFFIEPLVeky-----gpfSGIYCNKADFsg---------eQIKLIYsnscdeecakyst 145
gi 74625279   85 PFYEWCKsqDIRVIILSSGMEPFIRALFeqylgkeeassIEIVSNDINV------------HPDGQWnivyh------dd 146
gi 81558025   82 EFVAFTKerGIPLYIVSGGIDFFVYPLLegl-----iepERIFCNGSDFsg---------eTIRITWphacdg----ecq 143
gi 81686407   80 EFIQFVNenNISFYVISGGMDFFVYPLLqgl-----ipkEQIYCNETDFsn---------eYITVNWphpcdr----lcq 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              #                           ### ##                                        
2FEA_B       146 nqcGCCKPSVIHELSep----------nqYIIXIGDSVTDVEAAKLSDLCFARdYLLNECREQnlnhlpyqdFYEIRKEI 215
2FEA_A       146 nqcGCCKPSVIHELSep----------nqYIIXIGDSVTDVEAAKLSDLCFARdYLLNECREQnlnhlpyqdFYEIRKEI 215
gi 398365467 144 spfGHDKSRSIDAYKkkfestlkageqrpVYFYCGDGVSDLSAAKECDLLFAKrGKDLVTYCKkqnvpfhefDTFKDILA 223
gi 24459281  151 ---GHDKSLAIKPTGnlpk------edrpVLFYCGDGVSDLSAARETDLLFAKyGHDLITYCEregapftvfKTFEDIHR 221
gi 159029056 140 -teLVAKALIMDKYSg------------vETIAIGDSVTDITMARKADLVFARdRLIDYLQAEnqpyipwdnFFEIREYL 206
gi 171988989 143 nqcGCCKTSIARKLKqd----------gdIIVTIGDSVTDFELAKQADHVYARdYLITLCEQHgiaytpfetFYDIVDHL 212
gi 254764008 145 ngcGMCKTTIIRRYDpa----------thFRIVIGDSITDLAGAKIADYVIARsFLADKAEELqlphstfatFHDVIRIL 214
gi 259509732 142 ndcGCCKPTIARQIVsp----------tdTLIVIGDSVTDFEIAKRADVVYARgQLISLCEAEgirhvpfetFYDISAYM 211
gi 218527838 146 qgcGCCKPSVMRKVAke----------ehFKIVIGDSLSDFEAAKQADIVLARdHLIQRCEELhvsykpfitFHDCLKIV 215
gi 74625279  147 shfGHDKSLTIRPYAqlpe------skrpHMVYCGDGVSDLSAAKETEHLFAKkGRDLIKYCErekisfsefETFADIHK 220
gi 81558025  144 ngcGCCKPSLLRKLArp----------dgYHVVIGDSITDLAVAKQADYVMARdFLLQKCQELglphapfatFFDVIDAL 213
gi 81686407  142 nhcGLCKSSLIRKLSdt----------ndFHIVIGDSITDLQAAKQADKVFARdFLITKCEENhisytpfetFHDVQTEL 211

                 ..
Feature 1          
2FEA_B       216 EN 217
2FEA_A       216 EN 217
gi 398365467 224 SM 225
gi 24459281  222 DI 223
gi 159029056 207 LL 208
gi 171988989 213 QT 214
gi 254764008 215 QQ 216
gi 259509732 212 KG 213
gi 218527838 216 QE 217
gi 74625279  221 DL 222
gi 81558025  214 QR 215
gi 81686407  212 KH 213

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