2HX1


Conserved Protein Domain Family
HAD_like

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cd07525: HAD_like 
Click on image for an interactive view with Cn3D
uncharacterized family of the haloacid dehalogenase-like (HAD) hydrolase superfamily
The haloacid dehalogenase-like (HAD) hydrolases are a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members include 2-L-haloalkanoic acid dehalogenase (C-Cl bond hydrolysis), azetidine hydrolase (C-N bond hydrolysis); phosphonoacetaldehyde hydrolase (C-P bond hydrolysis), phosphoserine phosphatase and phosphomannomutase (CO-P bond hydrolysis), P-type ATPases (PO-P bond hydrolysis) and many others. Members are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319827
View PSSM: cd07525
Aligned: 12 rows
Threshold Bit Score: 237.609
Threshold Setting Gi: 83593130
Created: 9-Apr-2008
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #####                            ##                                        
2HX1_A        15 KCIFFDAFGVLKTYNGllPGIENTFDYLKAqGQDYYIVTNDAsrsPEQLADSYHKLGLFsi-taDKIISSGx------IT 87
gi 83591854   22 DAFIIDLWGVIHDGTQayPGAAAALAALKAqGKRTVLLTNAPr-lSGSVIAQMEGLGLGra-lyDAVMTSGd-----aVN 94
gi 16124958   47 DVVLSDVWGVIHNGVAsfPEACEALTKWAQtKGPVVLISNSPr-pSHDVVAQLDALGVPrs-awQGFVTSGd------AT 118
gi 144900448  17 DAFILDLWGVVHDGVEayAGARDTLVALRTaGKQSLLLSNAPr-rAEALVEQLARMGIEra-lyDYVLSSGe-----aVH 89
gi 36958658   18 DAFLVDQFGVLRDGRGpyPGAAETLVRLKTaGKRIIILSNSGk-rSAENDRRLAELGFEpg-swDWFLTSGe------VA 89
gi 15604434   17 DVFLFDLWGVIIEGNHtyPGVVQNINKIIA-QKKVYFVTNAPr-nIFSLHKTIKSWGINal--pEMIISSGei----aVQ 88
gi 42567769   24 KAWLLDQYGVLHDGKKpyPGAISTLKNLATaGAKIVIISNSSr-rASTTMEKLKGLGFDps-ffTGAITSGe------LT 95
gi 83593130   45 DLIVLDAYGVLHEGAEpyPAALEAFAALRArGKAVCVVTNAV---THAPGDVAARLTAL-----GFPLDAGevvsgrsLL 116
gi 27382589   21 DVVLSDIWGVVHNGLEsfPEACEALHTYRSrGGTVILITNAPr-pADSVQRQLRKLGVAde-tyDAIVSSGd------LT 92
gi 15888027   16 DVVLSDVWGVLHNGVSafPDAAVALHEARKaGKTVVLITNSPr-pAPGVIAQLRVLGVPde-ayDRIITSGd------VT 87
gi 77462857   16 SALFCDLWGCLHDGKRpfAEAVEALRAFRArGGTVLLMTNAPr-pKPSVVRQLESIGVPpd-cyDEVTSSGd------AA 87
gi 385232954  16 DAAFVDLWGCMHDGIRalPDAVAAMQDFRKgGGTVVLVTNSPr-pRASVERQITEDFGIapdswDAIATSGd------AA 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2HX1_A        88 KEYIDl------kVDGGIVAYlGTANSANYLVsdgikXLPVSAIDDSn-------iGEVNALVLLDDegf-------nwF 147
gi 83591854   95 AELLRrddp-ffqGLGQACLFvGPERDTNVLT-----DTGVALVTDPa-------kAGFVLCTGPVSfd--------esV 153
gi 16124958  119 RALLKa-------NAPGKVWKiGPARDEVLYE-----GIDLVAAGCEd--------AGFISCTGLYEdev-------evP 171
gi 144900448  90 LELQArtdp-fyaGLGRNLYHmGPERDVNVFE-----GLDYVAVDLAh--------ADFILNTGPWDve--------etV 147
gi 36958658   90 WQLLNqeaa-ataGGTRKCLLvSRDGDLSPLN-----GLDLVRTDSGe--------AADIVLIAASEgdv-------ypL 148
gi 15604434   89 MILESkkr---fdITNPIIYH-LGHLENDIIN-----LMQCYTTDDIn-------kANISLITIYRDene------kldL 146
gi 42567769   96 HQSLQrrddpwfaALGRRCIH-ITWNDRGAISl---eGLDLNVVENVe-------eADFVLAHGTEAlglpsgsvsprtI 164
gi 83593130  117 PDLLAge-----qDQGSGIMV-LGSHTAPVQErf-pqAIAQDWTAEAld-----raRGFLLIDTNGWm-----------D 173
gi 27382589   93 RLYVAe-------HPGRKMFWlGPERDNSIYR-----GLDAVTAPLEe--------ADYIVCTGLYDdet-------etA 145
gi 15888027   88 RGLIA--------EGPKKVFLlGPERDMPLLE-----GLDVERVGEAe--------AQSVVCTGFFDdet-------etP 139
gi 77462857   88 QYALVt------gAVGRRVYHlGPQKDETFFTel-spDLQKVAATEApivrvpleeAEGIVCTGLFDdlt-------etP 153
gi 385232954  89 RTALFt------gVVGQKVWFvGQPHERIFLEppkvlDTPIDVTEVDld------qAEGIVCAGPADpm--------adP 148
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                  #                     
2HX1_A       148 HDLNKTVNLLRKr-TIPAIVANTDNTYPLTktDVAIAIGGVATXIESILGr-RFIRFGKPDSQXFXFAYDXLRqkxei-- 223
gi 83591854  154 ADYAALLEACAAq-GLPMVCANPDRAVVREg-KTVICAGALADFYAGLGQ--TVVSRGKPDPAIYRLALERLDlpa---- 225
gi 16124958  172 EDYRDRLKVAAEr-GLLFICANPDRVVQRGd-RLIYCAGALADLYESLGG--KVVMAGKPFGQIYDLAVAEAArllgrpv 247
gi 144900448 148 EDYVPAMKQALEr-RLPMVCANPDLVVMRQg-QPVVCAGALAERYAEMGG--IVSMRGKPDPAIYVQALKILGlpa---- 219
gi 36958658  149 AHYEDLLRPAARr-GVPCLCTNPDKVMLTPs-GQAFGAGRIAELYEGLGG--KVRWIGKPFPDIYDLALDFLGrpd---- 220
gi 15604434  147 NEFDELFKIIVQr-KIINICANPDLGINQHg-IYRYCSGYYAEKIKQLGG--KVIYSGKPYEEIYHKILKECSnip---- 218
gi 42567769  165 DELEKILEKSAAr-GLPMIVANPDYVTVEAn-VFHIMPGTLASKYEELGG--EVKSMGKPHKMIYESAIAIAGvnp---- 236
gi 83593130  174 DEPESRLGASLRanPRPLIVCNPDVTCPFLg-KLSYEPGYFAFRLAAEIPdlPLRFLGKPYGAIYDRVAARFPgia---- 248
gi 27382589  146 EDYRGMMLKAREr-KLTLVCANPDIVVERGd-RLIYCAGAIAELYRELGG--EVIFYGKPHRPIYERAMALAGerqghqi 221
gi 15888027  140 EDYTEMLKGFIAr-KVPMICANPDLVVERGe-RIIPCAGAMAAYYEQLGG--EVRIAGKPHAPIYEACLAAAKdvrga-f 214
gi 77462857  154 EDYRATLLYAKTq-GLKLLCANPDIVVDFGd-KRIYCAGAIAEAYDAMGG--QSLYFGKPHPPIYDLARRRLEalrpg-v 228
gi 385232954 149 DLYRPEFERAIAr-GLKLLCANPDIVVDRGh-SREWCAGKLAQIYTEMGG--ESLYFGKPHAPIYDLARRRLDklgrk-v 223
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                ##   ##                                                                 
2HX1_A       224 skreILXVGDTlHTDILGGNKf-GLDTALVLTGNtr----------------idDAETKIKstg---------------i 271
gi 83591854  226 -garVAAIGDGvHTDMPGARAa-GVDAVFVTGGLn-------------------AELLGIRhgeapdqa-----kvrall 279
gi 16124958  248 drarILCVGDGvITDVKGAHDq-KLACLFVAKGIh------------------gDKAVGPDgq----------------l 292
gi 144900448 220 --arVMAVGDAlHTDVRGANQagLAGAVFVTQGIh-------------------AKDLGIKpgdnptqdrldhfvaahgd 278
gi 36958658  221 -parVCAIGDSiEHDIAGATGa-GLGSVLVTTGIlehqse--------eerrrlFDEHGVT------------------- 271
gi 15604434  219 -knrMLMIGDTfYTDILAANRl-GFDSALVLTGNsreyhfecd---nihekldsLMEAAIKqs----------------i 277
gi 42567769  237 --seSIAVGDSlHHDIRGANVs-GIESIFITGGIhgnel----------gltsfDETASLDsvktlt--------akhna 295
gi 83593130  249 -rerILAVGDSpHTDVLGARSa-GMAALLVESGLfrgrd-----------tgrlLAECAIL------------------- 296
gi 27382589  222 drrrVLAIGDSvRTDLTGAREf-GIDCLFVTRGIh------------------aEEFEGLDql----------------- 265
gi 15888027  215 akdrVLAIGDGmPTDVKGAIAs-GLNLLYISGGIh-------------------AAEYTLNgqtdealln--aylkgqga 272
gi 77462857  229 padeILCVGDGiATDIRGAVAe-GLDSLFITGGLaasvfgengesldqdrlehwLAEAELS------------------- 288
gi 385232954 224 dhsrIICIGDGiITDVQGALSe-DYDCLYISGGLa-------------------AAETKTTyqpepaald--ayltstgl 281

                 ....*..
Feature 1               
2HX1_A       272 vPTHICE 278
gi 83591854  280 dAHALTP 286
gi 16124958  293 vPDAVHA 299
gi 144900448 279 vPAAAIR 285
gi 36958658  272 -PDFILP 277
gi 15604434  278 iPNFVID 284
gi 42567769  296 fPTYVLS 302
gi 83593130  297 -PDFIAP 302
gi 27382589  266 dPASVME 272
gi 15888027  273 aPGWWMP 279
gi 77462857  289 -PTLTIA 294
gi 385232954 282 sPDYTIG 288

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