3L5K


Conserved Protein Domain Family
HAD_AtGPP-like

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cd07529: HAD_AtGPP-like 
Click on image for an interactive view with Cn3D
subfamily of beta-phosphoglucomutase-like family, similar to Arabidopsis thaliana Gpp1 and Gpp2
This subfamily includes Arabidopsis thaliana AtGpp1 and AtGpp2, and Drosophila GS1-like protein (Dmel\Gs1l) of unknown function. AtGpp1 and AtGpp2 are constitutively expressed in all the Arabidopsis tissues and unaffected under abiotic stress. Overexpression of AtGpp2 in transgenic Arabidopsis plants increases the specific DL-glycerol-3-phosphatase activity and improves the plants tolerance to salt, osmotic and oxidative stress. It belongs to the beta-phosphoglucomutase-like family whose other members include Lactococcus lactis beta-PGM, a mutase which catalyzes the interconversion of beta-D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), Saccharomyces cerevisiae phosphatases GPP1 and GPP2 that dephosphorylate DL-glycerol-3-phosphate and DOG1 and DOG2 that dephosphorylate 2-deoxyglucose-6-phosphate, and Escherichia coli 6-phosphogluconate phosphatase YieH. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319831
View PSSM: cd07529
Aligned: 11 rows
Threshold Bit Score: 271.913
Threshold Setting Gi: 84027747
Created: 8-Apr-2008
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              #####                                                                     
3L5K_A        30 VTHLIFDMDGLLLDTERLYSVVFQeICNRYDKKYs-WDVKSLVMGKKAleAAQIIIDVLQLPMSke--eLVEESQTKLKE 106
gi 20129151   10 VTHVIFDCDGTLIDSEGIYLKTVQdLLAKYGKTYt-KVDQTQHMGMPVgtFSQHIVKDLKLPLSp---aEFQKEFEAAVD 85
gi 45550911   16 VTHCIFELDGLLIDSERLRTETVQrILDPYGHTYs-FDLKMRCMGKPDseQAALIVNTFNLPFSm---tEFENQQELQCR 91
gi 47226791    3 VTHVIFDMDGLLLDTERLYTVAFQeICDRFEKQYt-WAVKSSVMGRNAldACQIIRDTLDLPMTae--eLLIESRQILKR 79
gi 26453252   22 ITHVIFDMDGLLLDTEKFYTEVQEkILARYNKTFd-WSLKAKMMGRKAieAARLFVDESGISDSls-aeDFIVERESMLQ 99
gi 259016422   8 YMACLFDMDGLLVDSETIYTKTTNlILDRYGKDPlpISVKAQMMGRPGsaAAKVVIDWSNIPMTpq--qFVDEQQVIRAK 85
gi 74626747    3 PEACLFDMDGLLVDTESIYTKSTNiILKRYNKGPfsMEVKAKMMGRTSkeASRIFLDWSGIDLTce--eYIALQRETQAE 80
gi 84027747    7 VKACLFDMDGLLINTEDIYTETLNeTLAEFGKGPltWDVKIKLQGLPGpeAGKRVIEHYKLPITl---dEYDERNVALQS 83
gi 19864674    9 VTHCVFDMDGLLLDTERLYTVATEmILEPYGKTYp-FEIKEQVMGLQTepLARFMVEHYELPMSw---eEYARQQRANTE 84
gi 808355714   5 VTHVIFDFDGLLVDTESAYTEANMeLLRKYGHVFt-MDLKRRQMGKRHdeSIRWLINELKIGDLvtpeeYSRQYDELLIE 83
gi 75161678   14 ITHVIFDMDGLLLDTEKFYTEVQEiILARFNKKFd-WSLKAKMMGRKAieAARIFVEESGISDSls-aeDFLVERESMLQ 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                     ##                                      #          
3L5K_A       107 VFpt-aaLMPGAEKLIIHLRkhGIPFALATSSrsASFDMKTSRHKEFF----SLFSHIVLGDDPevqHGKPDPDIFLACA 181
gi 20129151   86 KSmgsvaLLPGVRDLILHLHeyRIPFCIATSSfrKLFKVKAESFKDIFl--aFHHVVCGDDPALgpgRGKPYPDIYLLAA 163
gi 45550911   92 GKmgfirLMPGVERLLHHLKafNIPMAIASGCcrDSFRIKTRRHSRPFd--vFHHVVLSGSDEEv-kRGKPAPDVFLTTA 168
gi 47226791   80 IFps-akLLPGVEKLVIHLQqhNIPIAVATSSegVTFSLKTSQHKDFFg--rFHHIVLGDDPDVk--NNKPLPDSFLVCA 154
gi 26453252  100 DLfptsdLMPGASRLLRHLHgkGIPICIATGThtRHFDLKTQRHRELFs--lMHHVVRGDDPEVk--EGKPAPDGFLAAS 175
gi 259016422  86 FWss-lkPMPGAESLINNLSnhGIDIGLATSSntANYNMKTAHLKHIFek-fGKNVITGDNPSIapgRGKPFPDIWLKVL 163
gi 74626747   81 LWrh-tkPLPGVMNLLSKLKslNIPIALATSSd-THNFEKKSAHLSHLfdhfDGNIITGDDPRLpvgRGKPHPDIWFIAL 158
gi 84027747   84 LKwgtceFLPGALNLLKYLKlkNIPIALCTSS--NKTKFRGKTSHLEEgfdlFDTIVTGDDPRIakgRGKPFPDIWQLGL 161
gi 19864674   85 ILmrnaqLMPGAERLLRHLHanKVPFCLATSSgaDMVELKTAQHRELFs--lFNHKVCGSSDKEv-vNGKPAPDIFLVAA 161
gi 808355714  84 MFkr-spAMPGAEKLVRHLLhtGVPVALCTGScsRTFPTKLDNHKDWVnm-iKLQVLSGDDPEVk--HGKPHPDPFLVTM 159
gi 75161678   92 DLfptseLMPGASRLIKHLHvkNIPICIATGThtRHYDLKTQRHRELFs--lMHHVVRGDDPEVk--QGKPAPDGFLAAA 167
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
Feature 1                               ##  ##                
3L5K_A       182 KRFSPppa--------meKCLVFEDAPNGVEAALAAGMQVVMVPD 218
gi 20129151  164 SRFNPpad--------pkKCLIFEDAPVGLIGGKAAGSQVIFIPT 200
gi 45550911  169 SRFEEspe--------psKCLVFESSLVGMEAALSAGMQVVLVPD 205
gi 47226791  155 SRFNPpaapen-dwgndtRCLVFEDAPNGVTAALAAGMQVVMIPD 198
gi 26453252  176 RRFEDgpv-------dprKVLVFEDAPSGVQAAKNAGMNVIMVPD 213
gi 259016422 164 NLINEsrkqrglkaltpsQCIAFEDSIPGVKSAKAAGMHVIWVPD 208
gi 74626747  159 KMINDkrkaqgqaeilpeNCLVFEDSITGVQSGRAAGMKVVWVPD 203
gi 84027747  162 KELNEkfhtd----ikpdECIVFEDGIPGVKSAKAFGAHVIWVPH 202
gi 19864674  162 GRFGVppk--------psDCLVFEDSPNGVTAANSAGMQVVMVPD 198
gi 808355714 160 KRFPQvpe-------sadKVLVFEDSYNGVLSALDAGMQCVMVPE 197
gi 75161678  168 RRFKDgpv-------dsqKVLVFEDAPSGVLAAKNAGMNVVMVPD 205

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