Conserved Protein Domain Family
HAD_PNPase_UmpH-like

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cd07532: HAD_PNPase_UmpH-like 
UmpH/NagD family phosphatase para nitrophenyl phosphate phosphatase, similar to Plasmodium falciparum PNPase
Plasmodium falciparum para nitrophenyl phosphate phosphatase (PNPase) catalyzes the dephosphorylation of thiamine monophosphate to thiamine, other substrates on which its active are nucleotides, phosphorylated sugars, pyridoxal-5-phosphate, and paranitrophenyl phosphate. This subfamily belongs to the UmpH/NagD phosphatase family, and to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319834
View PSSM: cd07532
Aligned: 11 rows
Threshold Bit Score: 398.986
Threshold Setting Gi: 71984613
Created: 13-Jun-2008
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   #####                            ####                                
gi 24656326   19 QWLStFESVILDADGVLWHFskaIDGAVDTFNYMnt-tGRKIFIISNNSEisRQEMADKAKGFGIEIKe---DNVLTSSf 94
gi 22026920   19 EWLAgIDTIICSTDGVLWQEntpIEGSVEAFNAIis-kGKRCLIATNECCltNKDLFQKAKCLGFNVKe---QDIFSSSg 94
gi 24666137   19 EWLQsFDTVLCDGDGTIWQDdtaIAGAPDVVNALqdrfDKKVYLITNNGLktRQELFERSQRLGFHLPs--dRHIISPTa 96
gi 24666141   34 EWLAgFDSVITDCDGVLWIYgqaLEGSVDVMNQLkg-mGKSIYFCTNNSTktRSELLKKGVELGFHIKe---NGIISTAh 109
gi 17558880   51 KVMKtIDTFIFDADGVLWLGesvMPGSPRLIDYLvk-hNKQIIVLTNNATksRAVYAKKLAKLGYNSSkmnkNNLVNPAa 129
gi 24656330   26 QWLKtIDTIIFDGNGVLWSHgkvLENAAETFNALra-mGKKAFICTNNSVtsVEGICKYAQEMGFLVAk---NEILSSVq 101
gi 190624332  19 KWVKnIETIICDADGVLWHFtkaIDGAPEVFKRVte-sGRNLFIVTNNSSmpSEAFAKRAQGLGFMIDe---DHCRTSSt 94
gi 194113907  19 QWLGsIETVLFGTDGVLWNFddpIKGSVETFNATrn-kGKRCFLVTNDSSmvASDMAQKAMCLGLKVGe---QEILTSAa 94
gi 190620207  19 KWLAkIDTVVFGTDDVLWKGnepVDHAVEAFNLMkm-kGKRTLIVSNNTIlhSSELLKKAKKLGFNVEk---EEVLTSGg 94
gi 71984613   10 QLLSnFDTFVFDADGVLWTGdipIPGAAEWINTLlddpEKSVFITTNNSTktLEQYMKKVKKMRFGRLg--rENLLSPTi 87
gi 86563050   10 ELLAnYDTFLFDADGVLWTGdipVPGAIEWINLLledpSKKVFVLTNNSTktLEQYMKKIEKLGFGHLg--rNNVISPAi 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 24656326   95 SCANFLAvknf----qkkVFVMGEKGVHFELEkfgicslkmseklek--------pmhefvteleldpdvGAVIVGRDEg 162
gi 22026920   95 AIASYLSdrkf----kkkILVLGGDGIRKDLKeagfcsvvndlqpndq-------kkidfvrslvldpdvGAVLVARDDn 163
gi 24666137   97 AIADYLVgspkfdrtrhkVYVVGNAAIARELRqrgidsygaggtdelppgdkwpdfvtrefgnpeaakdvGAVVVGWDEy 176
gi 24666141  110 ATAAYLKrrnf----skrVFVIGSEGITKELDavgiqhtevgpepmkg------slaefmaqhlkldtdiGAVVVGFDEh 179
gi 17558880  130 VVADTLHragl---dgkrVYLIGEQGLRDEMDelgieyfghgpekkqde----adgsgafmydikleenvGAVVVGYEKh 202
gi 24656330  102 TLAKFMKekkf----kkkCYVVGGQGIVDELKlvgieslpldhsslqg------fsmpdhihsiyldpnvGAVVVGSDKd 171
gi 190624332  95 SIANFLKnkgm----rrkVFVMGEIGIRAELDkvgiahmevdekldk--------smyefakeleidpdvGAVVIGRDEr 162
gi 194113907  95 CISNYLVvkkf----kkkVLVVGETGIQEELQkagiqsvtidqeaeeh-------kmgpfarnlivdpdvGAVVVGRDKs 163
gi 190620207  95 LVVKYLKergi----egkVLVCGSKGLELEVQdagfntdienrdpegn-------nalehamntikdpevRVVLVGQDEq 163
gi 71984613   88 VLCDYFKqnsdk-fenqyIYLIGVENLKKSLEegggvkcfgtgpdhkdn----ytdgdfinevdvkskipKAVVVSFDSh 162
gi 86563050   88 VLADYLKsnadk-fsgeyVYLIGTENLKATLEndggvkcfgtgpdsird-----htdgdfihkvdmsiapKAVVCSYDAh 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                  #                                   #                 
gi 24656326  163 fNMAKLVRTGSYLln-pdVIFLGTCLDAAYpign-nrvMVGAGATLAAMKAYTGRSP-LVLGKPNPWMASTLMqs---ga 236
gi 22026920  164 mIANELLVACNYLqn-pkVLFLTTCIDGFQpfg--kkrIPDAGSLASAIEIIVQRKP-IVLGKPNQRILGKLMks---ge 236
gi 24666137  177 fSYCKMARACHILcsnpdAAFLVTNRDAVHkyp--sfcIPGTGAFVAGIEACSEREA-LEMGKPNPLVLEPFIka---eg 250
gi 24666141  180 fSFPKMMKAASYLnd-peCLFVATNTDERFpmp--nmiVPGSGSFVRAIQTCAERDP-VVIGKPNPAICESLVte---kk 252
gi 17558880  203 fDYVKMMKASNYLre-egVLFVATNEDETCpgpnpevvIPDAGPIVAAIKCASGRDP-LTVGKPCTPAFNYIKrk---wn 277
gi 24656330  172 fNTIKLTKACCYLrd-seVMFVATSRDAALpaap-grmVPSAGVMVAAIQAASQRMP-FTCGKPNPYMCIDLMqk---gv 245
gi 190624332 163 yNMARLIRTSAYLrn-pdVIVVGTSMDAAYpfdehrkvIVGASAMMTSVRALSGRQP-LILGKPNPWILDPLLkc---gv 237
gi 194113907 164 fNVSKIVVACTYLln-pkVMFLGTCMDTIYpvce-krvTVGAAAMVAAIEKSSNRKP-LIMGKPNPQMVYKLRqs---gv 237
gi 190620207 164 mDSRKMIVACNYLln-teILFLATGMDNFAnag--eyrIPDAYCMVQAIESVNHRKP-IILGKPNPQILGDLAs-----e 234
gi 71984613  163 fSYPKLMKAANFLad-plVEFLVCNEDSTFpgpvpgmiLPETGPWSAAIQNVSGRKPdIVFGKPHEQLANFLKsrvqagk 241
gi 86563050  162 fSYPKIMKASNYLqd-psVEYLVTNQDYTFpgpvpgvvIPGSGATSAAVTAVTGRDP-KVFGKPHKPMADFLLrr---ah 236
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 ###  ##                                                      
gi 24656326  237 IKPETTLMVGDTLQTDMHFASNCGFQSLMVGSGVNTPKevqqiieeg----dpkkkilvPDTYLPSLGHM 302
gi 22026920  237 IKPEKTLVIGNSLKSDILFASICGFQSLLVGCDNGAIEkaekikkeg----dekkmklvPDAFLSGLASF 302
gi 24666137  251 LRTERTLMIGDCLKIDVGFASNCGMLSLLVGTGRYNNLsdvrle---------kdrlpqPDFYLPRLGDL 311
gi 24666141  253 IDPSRTLMIGDRANTDILLGFNCGFQTLLVGSGIHQLKdverwklsq----dpeekkliPDVYLPKLGDL 318
gi 17558880  278 INPSRTMMIGDRTNTDVKFGRDHGMKTLLVLSGCHQIEdiienqm-------nerddmvPDYVAPYLGAL 340
gi 24656330  246 IQPDRTLIIGDTMCTDILLGYKCGFQTLLVGTGVNSYQdaieaqgsk----apllyqqvPDLYMPKLSNL 311
gi 190624332 238 IKPDTTLMVGDTMTADMKFAHNCGFHSLLVGTGVHSFEdaqkikdsg----dkkkksfiPDTYLPSFGHL 303
gi 194113907 238 LKPEKTLVIGDRLSSDIIFANNCGFKSLLVGSGAGSLEeaqqlkmeg----nekklmmvPDTFLPSLGHL 303
gi 190620207 235 LKPESTLVIGNSLKSDILFANLCKFQSLLIGCENGKLTkifkiikek----daskmdlvPDTFLSTLSPI 300
gi 71984613  242 FNSERTVMFGDRLDTDMMFGKNNGFTTVWMQTGVNTLLdielark--------ngestkIPDFTCRFSEF 303
gi 86563050  237 VDPKRTVMFGDRLDTDIMFGNANGQLSATPIQCQNESEnsepqkqgwisrllkgqsmdpSSWQKQSHSSL 306

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