2I33,3ET5,3PCT


Conserved Protein Domain Family
HAD_CAP

?
cd07534: HAD_CAP 
Click on image for an interactive view with Cn3D
molecular class C acid phosphatases, similar to Haemophilus influenzae e (P4) acid phosphatase; belongs to the haloacid dehalogenase-like hydrolase superfamily
Molecular class C acid phosphatases (CAPs) are nonspecific acid phosphatases with generally broad substrate specificity and optimum activity at neutral to acidic pH. Members include Haemophilus influenzae lipoprotein e (P4), Elizabethkingia meningosepticum OlpA, Helicobacter pylori HppA, Enterobacter sp. 4 acid phosphatase PhoI, and Streptococcus pyogenes M1 GAS LppC. Lipoprotein e (P4) exhibits phosphomonoesterase activity with aryl phosphate substrates including nicotinamide mononucleotide (NMN), tyrosine phosphate, phenyl phosphate, p-nitrophenyl phosphate, and 4-methylumbelliferyl phosphate. The role of P4 in NAD+ uptake appears to be the dephosphorylation of NMN to nicotinamide riboside, which is then taken up by the organism. Elizabethkingia meningosepticum OlpA is a broad-spectrum nucleotidase with preference for 5'-nucleotides, it efficiently hydrolyzes nucleotide monophosphates, with a strong preference for 5'-nucleotides and for 3'-AMP; OlpA can also hydrolyze sugar phosphates and beta-glycerol phosphate, although with a lower efficiency. Helicobacter pylori HppA is also a 5' nucleotidase. Members of this family belong to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
?
PSSM-Id: 319836
View PSSM: cd07534
Aligned: 13 rows
Threshold Bit Score: 236.846
Threshold Setting Gi: 56707745
Created: 12-Mar-2008
Updated: 18-Aug-2016
Structure
?
Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                           #####                        
2I33_A        30 AGEMKALYYQGYNTGQLKLDAAlak----------------gteKKPAIVLDLDETVLDN---SPHQAMSVKtgkg--yp 88
3ET5_A        31 SGEYKALAYQAYNAAKVAFDHAkva-----------------kgKKKAVVADLDETMLDN---SPYAGWQVQnnkp-fdg 89
3PCT_A        30 SGEYAALAHQAFNSAKMAFDHAkak-----------------kgKKKAVVVDLDETMIDN---SAYAGWQVQsgqg-fsp 88
gi 13622916   64 AAETQALYLQGYQLATDRLKEQlnk----------------ptdKPYSIVLDIDETVLDN---SPYQAKNVLegtg-ftp 123
gi 88194087   67 SAEAKALYLQGYNSAKTQLDKEikkn---------------kgkHKLAIALDLDETVLDN---SPYQGYASIhnkp--fp 126
gi 21233557   76 SEEYRAVAEQTYRAAAETLDAAlkqpnwdalv--peergnaatgLKPAVVLDVDETVLDN---SPYQARLVRdgke-yde 149
gi 296102303  42 SAEIMALQLQTYRFATGRFDEKvkdl---------------ktpENYAVVMDLDETVLDN---TPLLVRDMEqchdytqw 103
gi 37089374   38 SAEIRALQLQSYKMAKMALDNNlkl----------------vkdKKPAVILDLDETVLNT---FDYAGYLVKncik-ytp 97
gi 2059351    39 AGEYKALTIQAYQLAQIRLAQIltqe---------------vseKPRAIVLDIDETVLDN---SPYQAYQIEnkkn-fnq 99
gi 83815702   68 AVEYEGTTRQAYRMAEIMMRRAladslwtasleqanrgsdtysdKPPAVVLDVDETVLDN---SPYQARLVRddaa-ysa 143
gi 2943981    47 SGEYQALTWQAFNTARMAFDQApsl-----------------tgKPKAVIVDLDETMLDN---SAYSAWQAKngqp-fss 105
gi 4586218    48 SAEYQALCIQAYNIATERVDALpaer--------------kqgdRPYAIVTDIDETILDNtpnSVYQALRGKdyd----e 109
gi 56707745   38 SDEKRAIYLEIYNLAVHRIKHQvkee--------------hlkkGTWGVILDIDETALDN---SWLEYDNYKnysy--se 98
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                           ##                                           
2I33_A        89 yKWDDWINKaeaealPGSIDFLKYTESKGVDIYYISNRkt---nqLDATIKNLERVGAPqa-tkEHILLQdpk------- 157
3ET5_A        90 kDWTRWVDArqsravPGAVEFNNYVNSHKGKVFYVTNRkds--seKAGTIDDMKRLGFNgv-eeSAFYLKkdk------- 159
3PCT_A        89 kTWTKWVDArqsaaiPGAVEFSNYVNANGGTMFFVSNRrdd--veKAGTVDDMKRLGFTgv-ndKTLLLKkdk------- 158
gi 13622916  124 eSWDYWVQKkeakpvAGAKDFLQFADQNGVQIYYISDRst---tqVDATMENLQKEGIPvqgrdHLLFLEkgv------- 193
gi 88194087  127 eGWHEWVQAakakpvYGAKEFLKYADKKGVDIYYISDRdke--kdLKATQKNLKQQGIPqa-kkSHILLKgkd------- 196
gi 21233557  150 lSWDQWVAEkkakpiPGVVDFAKAANARGITLIYISNRav---hlKDATLANLRSAGLPva--dDSVFLGlgtvvpgceq 224
gi 296102303 104 dTWSDWEKQgkpgliPGAKAFLEHVNQSKVRIYYVSDRmq---enKADTIKTLKSLGLPqv-sdDSVLLDtv-------- 171
gi 37089374   98 eTWDKFEKEgsltliPGALDFLEYANSKGVKIFYISNRtq---knKAFTLKTLKSFKLPqv-seESVLLKekg------- 166
gi 2059351   100 eDWSKWTRLaqaepiAGALNFLNFTKNNGVEIFYVSNRse---aeRVPTLENLQKKNFPya-dnDHLILKtdk------- 168
gi 83815702  144 eSWANWVREeqaapvPGARAFTQAATAQGVQVIYLTNRda---slEAATRTNLRNLGFPvddapDAVLTQgere------ 214
gi 2943981   106 kTWSAWTQArqakavPGAVEFARHVTENGGTLFYVSNRdq---kdYAATVANMQQLGFPnv-sdKTVRLNtds------- 174
gi 4586218   110 eTWGKWCAQadadtlAGALSFFLHAANKGIEVFYVTNRrd---nlREQALQNLQRYGFPfa-deEHLLTThgp------- 178
gi 56707745   99 eKFRQGIIEqkakglPGAAKLTNLVHKLGGYVSFVSNRygadpeiIKATEENLTKEDIYy---dQILFYNekak------ 169
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #                               ##  ##                                      
2I33_A       158 -ekgKEKRRELVSqt--------------hdIVLFFGDNLSDFTGFDGks---vKDRNQAVtdskaqFGEKFIIFPNPMY 219
3ET5_A       160 --saKAARFAEIEkqg-------------yeIVLYVGDNLDDFGNTVYgk--lnADRRAFVdqnqgkFGKTFIMLPNANY 222
3PCT_A       159 --snKSVRFKQVEdmg-------------ydIVLFVGDNLNDFGDATYkk--snAERRDFVaknskaFGKKFIVLPNTQY 221
gi 13622916  194 --ksKESRRQKVKet--------------tnVTMLFGDNLLDFADFSKks---qEDRTALLsdlqeeFGRRFIIFPNPMY 254
gi 88194087  197 -dksKESRRQMVQkd--------------hkLVMLFGDNLLDFTDPKEat---aESREALIekhkddFGKKYIIFPNPMY 258
gi 21233557  225 ngseKNCRRRLAGek--------------yrVLMQFGDQLGDFVQVTAnt---gTARGALLqqyhdwFGERWWMLPNPSY 287
gi 296102303 172 ---sKEERRQSILkk--------------qqIIMLFGDSLPDFAVQFKnkk-psEQQRELVeasaehFGNDWIVLPNAAY 233
gi 37089374  167 --kpKAVRRELVAkd--------------yaIILQVGDTLHDFDALFAkdaknsQEQRAKVlqnaqkFGTEWIILPNSLY 230
gi 2059351   169 --ssKESRRQKLSek--------------ynIVLFFGDNLSDFSDMYYynn-egKTSSEKVlehpelFGSKFIILPNAMY 231
gi 83815702  215 gwtpKAARRRWVAer--------------yrILLLVGDNFGDFVAAADts---vSARRVKArsfrkyWGTRWIVLPNPQY 277
gi 2943981   175 --snKQARFDAIKnag-------------ynVVLYVGDNLNDFGGATWhk--gnQTRRDFVnlnhqqFGTQFIVLPNPLY 237
gi 4586218   179 --sdKEPRRLKIQeq--------------yeIVLLIGDNLGDFHHFFNtk--eeSGRKQALgltageFGRHFIMLPNPNY 240
gi 56707745  170 npkdKNSRFEAVKsgkytddiivtkklpahaVIAYFGDNIQDFPQMTQk-----NMRNVDNhky-tiFGEKYYIFPNPMY 243

                 ....
Feature 1            
2I33_A       220 GDWE 223
3ET5_A       223 GGWE 226
3PCT_A       222 GDWE 225
gi 13622916  255 GSWE 258
gi 88194087  259 GSWE 262
gi 21233557  288 GGWE 291
gi 296102303 234 GSWS 237
gi 37089374  231 GTWE 234
gi 2059351   232 GDWE 235
gi 83815702  278 GSWE 281
gi 2943981   238 GDWE 241
gi 4586218   241 GSWE 244
gi 56707745  244 GSWQ 247

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap