4FYP


Conserved Protein Domain Family
HAD_VSP

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cd07535: HAD_VSP 
Click on image for an interactive view with Cn3D
vegetative storage proteins similar to soybean VSPalpha and VSPbeta proteins; belongs to the haloacid dehalogenase-like superfamily
Soybean [Glycine max (L.) Merr.] vegetative storage protein VSPalpha and VSPbeta levels were identified as storage proteins due to their abundance and pattern of expression in plant tissues, they accumulate to almost one-half the amount of soluble leaf protein when soybean plants are continually depodded. They possess acid phosphatase activity which appears to be low compared to several other plant acid phosphatases, it increases in the leaves of depodded soybean plants, but to no more than 0.1% of the total acid phosphatase activity in these leaves. This acid phosphatase activity has maximal activity at pH 5.0 - 5.5, and can liberate Pi from different substrates such as napthyl acid phosphate, carboxyphenyl phosphate, sugar-phosphates, glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, phosphoenolpyruvate, ATP, ADP, PPi, and short chain polyphosphates; they cleave phosphoenolpyruvate, ATP, ADP, PPI, and polyphosphates most efficiently. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Soybean VSPalpha and VSPbeta lack this active site aspartate, other members of this family have this aspartate and may be more active. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319837
View PSSM: cd07535
Aligned: 13 rows
Threshold Bit Score: 230.341
Threshold Setting Gi: 18405204
Created: 12-Mar-2008
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                            #####                       
4FYP_A        68 VEDYLITSKQyqyds-------ktvnKEAYFYAkgla----lknDTVNVWIFDLDDTLLSSIPYyakygyg-tentapgA 135
gi 134145     68 TKEYIHGEQYrsds--------ktvnQQAYFYArdl------evHPKDTFVFSIDGTVLSNIPYykkhgyg-vekfnstL 132
gi 4204285    81 IKDYMTSSQYkddvartvdevilhfgSMCCSKSk---------cDGMDAWIFDIDDTLLSTIPYhkkngffggeklnstK 151
gi 20218801   12 VEKYMKTGGQywedsn-----vavvnILKYAESltl------agDGMDAWVFDADETLLSNIPYyenyeyg-glafdskT 79
gi 3786012    64 VEVYMLAGQYdr------------dvQLTVDQIkvylneiilpgDGMDAWILDVDDTCFSNVFYyrlkryg-cdpydptG 130
gi 9049468    85 VENYMRGHHYrrdsk------vvvdeAAAYAEAavlsg--dpaaDANATWVFDVDETALSHVKFykkhgfg-yhrtdepA 155
gi 15233504   69 VAEYLNGDQFlsdys------vivdyALAFAKSvei------sgDGKDVWIFDIDETLLTNIDYykahgyg-sepyddnK 135
gi 116784482  89 VKDYMDGSQYlldsn------vvanvSIAYANSlnl------sgDGKDVWVFDVDETLLSNLPLyaaynyg-gadmddgA 155
gi 125555731  77 VRAYMEGERYasdsa------vaaadSLAFAARalas----gggGARPAWVFDVDETLLTNAPYyavngwg-slefnetS 145
gi 18405204  114 ALHRIKQGIYlreln------ftiqmALTYFQTikp------mnDNCDVVVIDIDDTNLLEQDS---------------Y 166
gi 12643238   78 VEDYLITSKQyqyds-------ktvcKEAYFYAkgla----lknDTVNVWIFDLDDTLLSSIPYyakygyg-tektdpgA 145
gi 130718     69 VKEYMVGPGYkmeid------rvsdeAGEYAKSvdl------gdDGRDVWIFDVDETLLSNLPYysdhryg-levfddvE 135
gi 134146     69 TKDYINGEQFrsds--------ktvnQQAFFYAser------evHHNDIFIFGIDNTVLSNIPYyekhgyg-veefnetL 133
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          ##                                          # 
4FYP_A       136 YWSWLESgesTPGLPETLHLYENLlelGIEPIIISDRw--kKLSEVTVENLKAVGVTKWKHLILKpngsk--ltqvVYKS 211
gi 134145    133 YDEWVNKg-nAPALPETLKNYNKLvslGFKIIFLSGRt--lDKQAVTEANLKKAGYHTWEKLILKdpqdpstpnavSYKT 209
gi 4204285   152 FEDWIQKk-kAPAVPHMKKLYHDIrerGIKIFLISSRk--eYLRSATVDNLIQAGYYGWSNLMLRgledq-qkevkQYKS 227
gi 20218801   80 FDAWVLEm-kAPALPSSLLLYDRLsthGFQIFTLTGRd--eAQRNISVQNLVEAGYKGWAGLILReesdq-gtsasVYKP 155
gi 3786012   131 FRTWAMKg-eSPAIQPVLELFYKLietGFKVFLVTGRde-eTLRQATLENLHNQGFTGYERLIMRtadnk-rqsatTYKT 207
gi 9049468   156 FMEWLIAg-rASALPNTVTLYKKLlllGVKIVFLSDRpdtpELRNATATNLIKEGFDCWDELILRsenstatgsvvEYKS 234
gi 15233504  136 FSEWVEQg-tAPAFDASLRLYNALkklGFTIILLTGRd--eHQRTSTETNLRDAGYSGWERLLLRgpndq-gksatNYKS 211
gi 116784482 156 FIKWADLa-eAPALPASQRLYAHLlqlGFKIFLLTGRy--dYERNATEKNLVQAGYHSWEALLLRgpddy-ettavVYKS 231
gi 125555731 146 FDEWVDVa-kAPALPASLKLYNELqglGIHIILLTGRs--eFQRNATQVNLLFAGYHSWEKLILRqspdi-gktavQYKS 221
gi 18405204  167 YMKYIEEa-kHQKSILILALYSKLrsqGYSMVLLSRRp--eTERNATIEQLKSRGYSDWSHLIMS-----------REDT 232
gi 12643238  146 YWLWLGTgasTPGLPEALHLYQNIielGIEPIILSDRw--kLWKNVTLDNLEAAGVTYWKHLILKpngsn--lrqvVYKS 221
gi 130718    136 FDKWVENg-tAPALGSSLKLYQEVlklGFKVFLLTGRs--eRHRSVTVENLMNAGFHDWHKLILRgsddh-gktatTYKS 211
gi 134146    134 YDEWVNKg-dAPALPETLKNYNKLlslGFKIVFLSGRy--lDKMAVTEANLKKAGFHTWEQLILKdphli-tpnalSYKS 209
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
Feature 1                          ##  ##                      
4FYP_A       212 KVRNSLVk-kGYNIVGNIGDQWADLVEDT---PGRVFKLPNPLYYV 253
gi 134145    210 AAREKLIr-qGYNIVGIIGDQWSDLLGGHr-gESRTFKLPNPLYYI 253
gi 4204285   228 EKRKWLMs-lGYRVWGVMGDQWSSFAGCPl--PRRTFKLPNSIYYV 270
gi 20218801  156 KKRGELVk-kGYRLWGRVGDQWSDLSGPYe--ASRSFKLPNPMYYI 198
gi 3786012   208 RIRKEMMe-eGYRIWGNVGDQWSDLQGEYs--GDRTFKIPNPMYFV 250
gi 9049468   235 GERKKLEeekGMVIIGNIGDQWSDLLGSPe--GRRTFKLPNPAYYI 278
gi 15233504  212 EQRSKLIe-eGFKIRGNSGDQWSDLQGFAv--ADRSFKVPNPMYYI 254
gi 116784482 232 GRRLKIEk-dGFRIRGNSGDQWSDLSGYSc--GDRTFKLPNPMYFI 274
gi 125555731 222 ERRAALEa-eGFKILGNSGDQWSDLLGLPm--ATRSFKLPNPMYFI 264
gi 18405204  233 RQKEELEr--GHRVIGVIGNHMDVLRGQWnwqSKRLFKLPSLTYDD 276
gi 12643238  222 KVRKSLVk-kGYNIVGNIGDQWADLVEDT---PGRVFKLPNPLYYV 263
gi 130718    212 ERRNAMVe-eGFRIVGNSGDQWSDLLGSSm--SYRSFKLPNPMYYI 254
gi 134146    210 AMRENLLr-qGYRIVGIIGDQWSDLLGDHr-gESRTFKLPNPMYYI 253

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