Conserved Protein Domain Family
nitrilases_CHs

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cd07564: nitrilases_CHs 
Nitrilases, cyanide hydratase (CH)s, and similar proteins (class 1 nitrilases)
Nitrilases (nitrile aminohydrolases, EC:3.5.5.1) hydrolyze nitriles (RCN) to ammonia and the corresponding carboxylic acid. Most nitrilases prefer aromatic nitriles, some prefer arylacetonitriles and others aliphatic nitriles. This group includes the nitrilase cyanide dihydratase (CDH), which hydrolyzes inorganic cyanide (HCN) to produce formate. It also includes cyanide hydratase (CH), which hydrolyzes HCN to formamide. This group includes four Arabidopsis thaliana nitrilases (Ath)NIT1-4. AthNIT1-3 have a strong substrate preference for phenylpropionitrile (PPN) and other nitriles which may originate from the breakdown of glucosinolates. The product of PPN hydrolysis, phenylacetic acid has auxin activity. AthNIT1-3 can also convert indoacetonitrile to indole-3-acetic acid (IAA, auxin), but with a lower affinity and velocity. From their expression patterns, it has been speculated that NIT3 may produce IAA during the early stages of germination, and that NIT3 may produce IAA during embryo development and maturation. AthNIT4 has a strong substrate specificity for the nitrile, beta-cyano-L-alanine (Ala(CN)), an intermediate of cyanide detoxification. AthNIT4 has both a nitrilase activity and a nitrile hydratase (NHase) activity, which generate aspartic acid and asparagine respectively from Ala(CN). NHase catalyzes the hydration of nitriles to their corresponding amides. This subgroup belongs to a larger nitrilase superfamily comprised of belong to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 1.
Statistics
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PSSM-Id: 143588
View PSSM: cd07564
Aligned: 107 rows
Threshold Bit Score: 297.862
Threshold Setting Gi: 46109632
Created: 21-Apr-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                #                                       
gi 51701632    7 YRVAAVQASPVLLdLDATIDKTCRLVDEAaanGAKVIAFPEAFIPGYPWWiwlgna-----dygmkyYIQLYKNSVe--- 78
gi 111054396  13 VKVAVIQASSVIMdRDATTKKAVSLIHQAaekGAKIVVFPEAFIPAYPRGlsfgttigsrsaegrkdWYRYWSNSVa--- 89
gi 188585699   7 VRTAVIQAAPIIMdREKTLNKALDLIKEAkneQAEIVVFPEAFIPAYPRGlsfgylvgsrtmegrndFLRYYKNAVp--- 83
gi 124002421   4 LDIAVIQAAPVLFdLEQSLDKTYDLLKKAtaqGAKMVVFPESFLPAYPRGlsfgtvvgsrtdagrevWQLYWKNSVk--- 80
gi 160940247  20 CKIAVIQAAPVMFdKDACTQKAVDLIQEAsrrGAQLMVFPELFIPGYPYGmtfgfrvgsrsgegrqdWKLYLDNSIl--- 96
gi 229420984   8 CKIAVVQAAPILFnKDECVKKVIDYIEESaskGSELIVFPELFIPGYPYGmtfgftvgsrkelgrvdWKRYYDNSIv--- 84
gi 126647110   3 VKVCLVQESPVFFdKEKTLQKLERLCEKYakeGNQLIVFPESFIPGYPRGfsfgakigsrtpegrdlYEEYYQNSFd--- 79
gi 74966765    2 PKIAIVQAGTPLFdKPATLEKVKKNVEEAagnGAELVLFPEAFIGGYPKWnsfgitmgtrtpegrkeFKRYFENAIe--- 78
gi 46109632    7 IRLGTASPGTASS-TPETIALIDTLAQRAassHIDILLLPEAFIGGYPRGssfgckigdrsaagrdeFARYFDQAIdlgd 85
gi 240111865   5 LKVAVSQARTRDT-LQQTLEALERITRVAasrGARIILFPEGYLGGYPRTctwgaamggrddsgreqYLHYYQAAVdmgd 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       #
gi 51701632   79 ------------------------IPSLAVQKLSSAGTN-KVYFCVSVTEKD----GGSLYLTQLWF-DPNGdLIGKHRK 128
gi 111054396  90 ------------------------VPDETTEQLGEAARKaGVYLVIGVTERDnefsGGTLYCSVLFF-DSDGqLLGKHRK 144
gi 188585699  84 ------------------------VPGDTTEKLGEAARKaGIYLVIGVTEIDteknNNTLYCTILYF-GPDGrLLDKHQK 138
gi 124002421  81 ------------------------VPGKATNLLAKWAKEyAVYLVMGITEQDt--vNGSLYCSLLYF-SPEGhLLGKHRK 133
gi 160940247  97 ------------------------VPGKETEKIGMAAREaGAYVSIGVSERDg--vTGTLYNTNLFF-SPRGsLVCVHRK 149
gi 229420984  85 ------------------------IPGKETDAIAEVVKKyKVYVSIGVSERDl--eTATLYNSNIIF-SPEGeIVSVHRK 137
gi 126647110  80 ------------------------LDSEDLTRLEKLCHQlNIYLVCGVTERMk--qHGSLYCSMIYI-SPKG-LLGVHRK 131
gi 74966765   79 ------------------------ENGEESKLIESLAAQnNIHIVIGVVERE----ASTLYCSVFFY-SPSG-YLGKHRK 128
gi 46109632   86 tvgpagggagikwvkrqigeedevRGDGSREELERIARDsGVFVVVGCIEKA----GGSLYCSVVYVcPKEG-MLGKRRK 160
gi 240111865  84 tpagagddw--vsrrlpiaegknyRGDGTREVLERIAREtDMLIIVGLIERC----AGSLYCAVVYV-DPKRgVLGKRRK 156
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #  #                              ## ##                   #                  
gi 51701632  129 LKATNAEKTIWGDGDGSMMPVFET----EFGNLGGLQCWEHFLPLNVAAMasMNEQVHVASWpigmpqeghlfgpEQCVT 204
gi 111054396 145 LKPTAAERIVWGEGDGSTLPVFDT----PYGRIGALICWENYMPLARAAMyaQGIQIYIAPTad---------arETWQS 211
gi 188585699 139 LKPTGSERLIWGEGDGSTLSVVNT----PHGKLGGLICWENYMPLARTAMyqKGVSLYVAPTad---------arSVWQS 205
gi 124002421 134 LKPTAAERIIWGEGDATTLQTYPT----PYGNIGGLICWENYMPLARMALyqQGIHLYLAPTad---------arESWQA 200
gi 160940247 150 LKPTGAERVVWGDADRGYFPVEDT----PWGVMGSLICWESYMPLARAALyeKGVALYISPNtn---------dnPEWQS 216
gi 229420984 138 LKPTGSERVVWGDANKYYFPVCDT----PWGNIGSLICWESYMPLARVALyeKGITIYISPNtn---------dnKEWQD 204
gi 126647110 132 IKPTGIERLVWAEASGDSLVTFDT----KIGKLGGLICWENYMPLARMAMysQGVEIYIAPTad---------arESWVD 198
gi 74966765  129 LLPTALERCVWGQGDGSTMPVFST----SVGKIGSAICWENYMPLYRMTLysKEIQIYLAPTvd---------drDVWLS 195
gi 46109632  161 VLPTGTERLIWAQGSPSTLRAVTTvirgIRINLAAAICWESYMPLLRQSLysQNINLYLAPTad---------frDAWLS 231
gi 240111865 157 VMPTGTERLVWAQGSASTLKAVTTevdgVKLTLAAAICWENYMPLLRQSLyqQNVNLYLAPTad---------grDTWLP 227
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 51701632  205 ATKYYAISNQVFCLLSSQIWTEEqrdkicetee------qrnfmkvghGFSKIIAPnGMEIGNKLa----hDEEGITYAD 274
gi 111054396 212 TIRHIALEGRCFVLSANQYVTKDmypkdlacyde----lasspeimsrGGSAIVGPlGEYVAEPVf-----GKEDIIIAE 282
gi 188585699 206 TIRHIALEGRCYVLSCNQFVTKDmypddleyyhe----lqnqpnimcsGGSAIVNPmGEYVVEPVy-----NKEEMLLAD 276
gi 124002421 201 TMQHIALEGRCFVVGCNQFVTKSmypphlrelpe----mtsqpevmsrGGSVVLSPlGKVLAGPVf-----DREEVLLAT 271
gi 160940247 217 TVQHIALEGRCYFINCNMYITRDmypenlccrde----idglpdtvcrGGSCIVDPyGHYVTEPVw-----DKEAVIYAD 287
gi 229420984 205 TIKHIAIEGHCYFINCDMYFTKDdypkdllskee----idrldnivcrGGSCIVDPyGHYETEPVw-----DREEIIYAE 275
gi 126647110 199 TMRHIAIEGRCFVLACNQYFTKSmyphrlqkdm------eeveedfckGGTVIFSPlGELIAGPLy-----GEAGALSME 267
gi 74966765  196 TMRTIALEGRCFVVSACQFLKSSdypldhplrk-----ehgedkvlirGGSCAVDPlGTVLVEPDf-----TKETIRYTE 265
gi 46109632  232 LARTIGVEGRCFVVCSNMCVPKDasaetngng--------ggpavrerRGSCLTDE-GFEIALPKsp---tRSRRRRKSV 299
gi 240111865 228 LMQTVAFEGRAVVLTCNQCVRKSqlpswvkgddnaqkdssdpdpiltgGGSCIISPmGKVLAGPVwnvdddDEEGLQISE 307
                        330       340       350
                 ....*....|....*....|....*....|....*..
Feature 1                                             
gi 51701632  275 IDLE--QIIPGKf----lIDSAGHYSTPGFLSLSFDR 305
gi 111054396 283 LDMK--QIAYSQf----dFDPVGHYARPDVFKLLVNK 313
gi 188585699 277 LNMD--LVIKGKm----dFDVVGHYDRTDVFQLKINE 307
gi 124002421 272 LDLD--DIIKSKl----dFDPVGHYSRPDMLNFNISN 302
gi 160940247 288 LEMD--RVSASRm----eFDVCGHYSRPDVLRLQIDD 318
gi 229420984 276 LDMN--KVPMSRm----eFDVCGHYSRPDVLQLKVDD 306
gi 126647110 268 IDLN--LITKSKl----dFDPIGHYARDDIFEFKVKG 298
gi 74966765  266 FDLS--DLALGKm----dLDVVGHYSRPDVFQLKVNE 296
gi 46109632  300 FDEDgnEIVLGCeddgeaPPPPPPAARPQVAALKTSG 336
gi 240111865 308 ADFE--DCVRGRl----dLDVAGSYSRNDAFKLTVEG 338

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