Conserved Protein Domain Family
ALP_N-acyl_transferase

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cd07571: ALP_N-acyl_transferase 
Apolipoprotein N-acyl transferase (class 9 nitrilases)
ALP N-acyl transferase (Lnt), is an essential membrane-bound enzyme in gram-negative bacteria, which catalyzes the N-acylation of apolipoproteins, the final step in lipoprotein maturation. This is a reverse amidase (i.e. condensation) reaction. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 9.
Statistics
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PSSM-Id: 143595
View PSSM: cd07571
Aligned: 297 rows
Threshold Bit Score: 164.309
Threshold Setting Gi: 33861848
Created: 21-Apr-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                 #                      
gi 117648    222 IQVSMVQGDIPQslk------wdEGQLLNTLKIYYNATapl------mgkSSLIIWPESAITdleinqQPFLKALDGELr 289
gi 88809140  223 LNLALWQPAIPTrekf---saerQRQFPRLLRAALTRAea--------dgADLLIAPEGTLPl----dRGNLLAEPVPL- 286
gi 113952972 201 YSVGLWQPAIPTrekf---saqrQRDLPLRLQAALQEAea--------adADWLMAPEGTLSf----hDSLIAPAPIPL- 264
gi 159903880 227 KAVAIWQSAIPTrekf---sidqLRRLPFSLEQALKEAnv--------lgASFMVAPEGTLPi----gQELLSPAQIPL- 290
gi 148242886 227 LSLVVAQPNLPTrekfsaeqqqrQRQLLMKAMAAAEQQg-----------ADAVVLPEGALPl-----GQALPAMPVAL- 289
gi 78212420  218 VALGVWQPAVPT-----------REKFDLERQQALPMAlvdamrqlepnnPAAVVAPEGALP------ARFQLPAEAPAm 280
gi 33240815  230 KRIALWQTNIPTrqkf---tlreLKRLPISLQDALEEAdn--------lgADWMVAPEGTLSa----gQNLLAPSPLPL- 293
gi 72382698  226 EKVAMWQTNIPIrqkf---sqeeINALPSKVNRALTEAfe--------mnASFLIAPEGTLPl----dRSKIRDFSIKF- 289
gi 33861848  231 YPVALWQTNMPTrekt----nfkNQFINDKLISAQKIAls--------ndAKLLITPEGTLN------NNFNLNFKSKIr 292
gi 87301159  221 FRVLVLQPALPTrekf---epdrQRDLLLQIARARQQAat---------lGVPLLLPEGTLVl-----GQAMPEAEAEV- 282
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                     #   #   #                          
gi 117648    290 dkGSSLVTGIVDArlnkqnrydtYNTIITLGKGApysyesaDRYNKNHLVPFGEFVPLESILrplapffdlpmssFSRGP 369
gi 88809140  287 --ISGGFRWVTGRq---------RSALLLLQGDDat---paAVIDKHRLVPLGEWTPALPGLagls-----avggLEPGG 347
gi 113952972 265 --MSGGFRWSRGRq---------HSAMVLVEAGGtt---pvASIDKHRLVPLGEWVPALPGLsgls-----aiggLEAGE 325
gi 159903880 291 --LSGGFRADKGNq---------RSSLLVFKKDQtt---ftDFVDKYRLVPLGEWIPPLPGLsipgl---ssiggIEPGE 353
gi 148242886 290 --LSGGFRRQELDe---------RSSVLWFPAGStr---pqQWLDKQRLVPLGEWVPGGLAGlsa-------vggVSPGE 348
gi 78212420  281 plISGGFRWVRGQq---------RSSLLLYEPPDwf---pvPLADKHRLVPIGEWIPPLPAGltagl---savggLSPGP 345
gi 33240815  294 --LSGGFRRVKNKq---------MSSLLVFNEGSts---ysSAIDKHRLVPLGEWLPSLPGVnwngl---sfvggVDPGD 356
gi 72382698  290 --LSGGFRKINGSi---------RSSLLVFNPGEes---fsDVLDKSRLVPLGEWIPELPNFlkdgl---savggIESGN 352
gi 33861848  293 -mLAGGFRNSKNGl---------RSSLLGYQIGDkt---ysSFIDKHRLVPLGEKIPGFLNIfsrgl---savggIQPGS 356
gi 87301159  283 --LSGGFRLEGEEl---------RSSVLRFAPSAse---asGWVDKARVVPLGEWVPLARLWrwggl---savggVEPGP 345
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                          ## ##                    #                                    
gi 117648    370 yiqPPLSAng-iELTAAICYEIILGEqVRDNFrpdTDYLLTISNDAWFGKSiGPWQHFQMARMRALELARPLLRSTNNGI 448
gi 88809140  348 asrLWRWGg--pPAQVAICYEISNGTaLARAAaggGQWILTAANLDPYPLL-LQHQFLALAQLRSVETARPLVSAANTGP 424
gi 113952972 326 psrLWRWGg--pPASVAICYEISNGAaLARAVaegAEWILAAANLDPYPRL-LQMQYLALAQLRSLETARPLLSTANTGP 402
gi 159903880 354 ssrLLIWDg--pPIAVAICYEITNGNgLSKAVkdgAQWILAIANLDPYPIS-LQKQFLALAQMRSIESARDLITVSNTGP 430
gi 148242886 349 aprLLALPpplgPVAVAICYELSDGLgLNQAVrdgAGWLLAIANLDPYPLA-LQQQFTALAQLRAIETSRWLLSSANTGP 427
gi 78212420  346 aprTMAVVe--pPAAVAICYEIADGLaLARAAadgATWLLAIANLDPYPLQ-LQEQFLALAQLRAIETGRDLLSVGNTGP 422
gi 33240815  357 asrFFDWDg--gPLAVAICYELSDGNnLAKAIfdgAEWILAIANLDPYPIS-LQRQFLALAQLRSIESARNLISVANTGP 433
gi 72382698  353 psrLLDWEg--pSFAGAICYELSNGKaIAKAVnqgAKWILVIANLDPYPIS-LQKQFLSIAQLRSIETSKNLISVSNTGP 429
gi 33861848  357 dsrFFKWKft-qPLAIAICYEITDGFkIRNAVksgAELIISAANLDPYPRK-LHYQFLSLARVRSIENKKDNIIISNTGP 434
gi 87301159  346 psrLLRRSg--aDPAVAICYELANGEgLREATvagAGWLLASANLDPYPAL-LRDQFTALAQLRAIEVGRWLVSTANTGP 422
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*....
Feature 1                                                         
gi 117648    449 TAVIGPqGEIQAMIPqftrEVLTTNVTPTtgLTPYARtGNWPLWVLTAL 497
gi 88809140  425 TAAINAhGQVTSRLTamrpGVLTVQVQPStvMTTYARlREWPLVGLLLI 473
gi 113952972 403 TAMIRAdGQIADRLAsfdpGLLVVPFQPRtgLTGYVRwGELPLLLLIGA 451
gi 159903880 431 STMISAdGSITPVIPpfkqKVGLASLHFSekITPYVRwGEKPLIVFLIA 479
gi 148242886 428 SALISPrGELHQQLPanqeSLGLMQVPTLssESLLTRwGVWPQWLLWLG 476
gi 78212420  423 TALISAnGSVQRLLApeasGVAEAVVQVRqrVTPYSRwISPPPRTVSPS 471
gi 33240815  434 TSMILSsGKIKSIIEpfneGVGVIDINVSqkISGYVRwGEIPLISSLLI 482
gi 72382698  430 TSLIKSnGRIDTLLKpneeLVQLADLELNgkKTLYTLiSDLPMIVVILI 478
gi 33861848  435 SGLISEeGKIIKLFDpnteQNDVVNPNFSieKTFYTTyGERPLFLLCLF 483
gi 87301159  423 SLVIDGrGVVRAALPpghpELGVFELERLkrSTTYQIwREAPLILMILL 471

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