1EMS,1F89,1F89,2W1V


Conserved Protein Domain Family
nit

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cd07572: nit 
Click on image for an interactive view with Cn3D
Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)
This subgroup includes mammalian Nit1 and Nit2, the Nit1-like domain of the invertebrate NitFhit, and various uncharacterized bacterial and archaeal Nit-like proteins. Nit1 and Nit2 are candidate tumor suppressor proteins. In NitFhit, the Nit1-like domain is encoded as a fusion protein with the non-homologous tumor suppressor, fragile histidine triad (Fhit). Mammalian Nit1 and Fhit may affect distinct signal pathways, and both may participate in DNA damage-induced apoptosis. Nit1 is a negative regulator in T cells. Overexpression of Nit2 in HeLa cells leads to a suppression of cell growth through cell cycle arrest in G2. These Nit proteins and the Nit1-like domain of NitFhit belong to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 10.
Statistics
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PSSM-Id: 143596
View PSSM: cd07572
Aligned: 214 rows
Threshold Bit Score: 299.342
Threshold Setting Gi: 93005053
Created: 21-Apr-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               #                                        
1EMS_B        16 FIAVCQMTsd-nDLEKNFQAAKNMIERAGEkKCEMVFLPECFDFIGlnkneqid---lamatdceYMEKYRELARKHNIW 91
gi 126698064   5 KIAVCQMItt-eNKIENINHAVDMVTEAAInGAKIVVLPEMFNCPYenkyfpkf----aeeypgeTTTILSKLAEKHGIY 79
gi 148642560   4 KIALCQINvv-dNKEKNIENATSMILKAVKqNADFIVLPEMFNCPYsnekfiey---ceeethspTLSKIAKLANENNTY 79
gi 219854794  19 KIGLCQMMvlksSKSDNVKRAKEAIYKAANkGTDIVALPEMFNCYYntkyfreya--ekddckgeTLSMLSSAAKEMGIY 96
gi 225575099  39 KVALLQTHvs-eKKEENLSVVREKLRALRAeKPDLVTLPEMFNCPYqtdqfpvy----aepqggeSWQALSCMAKEEGIY 113
gi 153811658   5 KIAAIQMStv-aDKMENVRTVKTYLEKIKDeNPDFVILPEMFCCPYqtenfpiy----aekeggpVWQQLSGYAKQYGIY 79
gi 187779585   5 KIALCQMQvq-kEKKKNIEKAIEMLTKAKEeNCNIAILPEMFNCPYenkcfkpygeiineenggeTVKAIKKAANDLNLY 83
gi 169830751   5 KLGICQMPvt-aSKEQNLSRARQMAAEAARaGARVVVLPEMFNCPYqhefftrf---aetcpdgdTFRMLTSTARELGVY 80
gi 225389330   5 RAAMLQTRvf-aDKMENIREAGRKLEALEAeQVDLVTLPEMFNCPYqtpnfpvy----aepqggpAWTACADLARKHKIY 79
gi 220929143   6 KLGLCQMAvt-dSKNENVKKAVFMLEECCKrGADIAVLPEMFNCPYdtklfply---aenfenskTLSVISDSAKYNNMY 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                          #                #   #           #                         ## 
1EMS_B        92 LSlGGLHHKdpsdaaHPWNTHLIIDSDGVTrAEYNKLHLFDLEIPGkvrlMESEFSKAGTemiPPVDTpigRLGLSICYD 171
gi 126698064  80 LVsGSIPELed---gKIYNTCYVFDKNGTLiGKHRKMHLFDIEVTGkvsfKESDTLTAGNd-vTVIDTeygKVGIAICYD 155
gi 148642560  80 ILaGSIPEKeg---sKIFNTSYLFDKNGEIiAKHQKMHLFDIDVKGkiyfKESDTLSSGNk-vTIAKTdfgKVGIGICYD 155
gi 219854794  97 IVgGSIPEIdd--kgNIYNTSFVFNEKGELiGKHRKMHLFDIDIKDkitfKESNVLTPGDk-vTVIDTkwgKIGVAICYD 173
gi 225575099 114 LAaGSVPEVde--egKVYNTAYVFDRQGRQiAKHRKMHLFDINVTGgqyfKESDTLTAGDa-iTVFDTefgRMGICICFD 190
gi 153811658  80 LIgGSMPEKda--egNVYNTSYIFDREGKQiGKHRKVHLFDIDVKGgqtfKESDMLTAGDs-dTVFDTefgKIGVMLCFD 156
gi 187779585  84 IVaGSIPEIeg---dKVYNTSMIVDNKGALiTKHRKIHLFDIDVKGgvtfKESDTLTAGNk-iTLFDTpwgKLGVMICYD 159
gi 169830751  81 LIgGSIPEAed---gRTYNTCFVYGPDGRMlGRQRKLHLFNIETDDlv-fRESDTLSPGTgppTVFVTplvTFGVAICFD 156
gi 225389330  80 LSaGSMPEVde--tgRVYNTAYVFDRQGRQiAKHRKAHLFDIDVKGgqcfKESDTLTAGDq-vTVFDTefgTMGLCICYD 156
gi 220929143  82 IVaGTIPEFsn---gCIYNTSIMFDRQGKIiAKHRKIHLFDVNIKDgvsfRESDVLAAGRs-vTVAQTefgRIGLAICFD 157
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ##                    #                                                         
1EMS_B       172 VRFPELSLWNRkrgAQLLSFPSAFTlNTGLAHWETLLRARAIENQCYVVAAAQTGAHnpkrqsYGHSMVVDPWGAVVAQC 251
gi 126698064 156 IRFPELSRLMAlkgAEIVILPAAFNmTTGPAHWELSIRMRALDNQIFYVGAAPARNMnasyiaFGNSRISDPWGRIIAQA 235
gi 148642560 156 IRFPELAKLMAeagAQILFYPGAFNmTTGPAHWELTFRSRALDNQVYCVGVAPALNKdasyhsYGHSIIANPWGEVIVQL 235
gi 219854794 174 VRFPELMRLMAlqgAKIIFIPASFNtTTGPSHWELLFRSAAVENQLYTVGISPARNInysyvaYGNSLVVDPWGKILNIL 253
gi 225575099 191 IRFPELSRLMAqegARLILIPGAFNmTTGPAHWELSFRARALDNQVYMLGTAPARDEkssytsWGHSIVVNPWGEVQAQL 270
gi 153811658 157 IRFPELSRMMVndgAKVIFVPAAFNmTTGPAHWELSFRTRALDNQIYMVGCAPARDVsagyisWGHSIVTDPWGRVTGML 236
gi 187779585 160 IRFPELSRIMAlkgAKIIFTPAAFNmTTGPAHWDTLFKSRALDNQIYMVGVAPARNEnsnyisYGNSLIASPWGNIVGRL 239
gi 169830751 157 LRFPELFRDLAargAELIVAPAAFNtFTGPPHWELLLRARAVDNQVFVAGAGPAWTEdspypyYGHSLVVNPWAEVIACA 236
gi 225389330 157 FRFPELARLMVekgAKVILVPAAFNmTTGPAHWEILFRCRALDNQAFVLGTAPARDPssgytsWGHSIAVSPWGDILGQM 236
gi 220929143 158 MRFTELYSQMSeagAKIIITPASFNmTTGPVHWELLVRARALDNQIFHAAVSSARNTsdtyisYGNSMVCDPWGRVISKA 237
                        250       260       270
                 ....*....|....*....|....*....|
Feature 1                                      
1EMS_B       252 SErvDMCFAEIDLSYVDTLREMQPVFSHRR 281
gi 126698064 236 DEkeCIIYADIDRDLIPDIRQQLPLLKHRR 265
gi 148642560 236 DEqeNMEIAEIDLEEIKKVRTELPLLKNKR 265
gi 219854794 254 DEkeGILLSEIDLEYIYEVRKSIPVFEHRR 283
gi 225575099 271 DEkeGCLVQELNLDEIDRIRRDLPLLSSRR 300
gi 153811658 237 DEneGILLAELDMDYEEQVREELPLLKSRR 266
gi 187779585 240 GAeeNILFSEIDLDYENKIREELPLLRHIR 269
gi 169830751 237 GEreTVLLAEIDLGQVAEVRKKLPLHRTEK 266
gi 225389330 237 DEreGCMIHTLDLERAGEVRNQLPLLKHRR 266
gi 220929143 238 DEkeGILIADIDLNMVNSVRSQIPVNKIID 267

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