1J31,1J31


Conserved Protein Domain Family
Ph0642_like

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cd07577: Ph0642_like 
Click on image for an interactive view with Cn3D
Pyrococcus horikoshii Ph0642 and related proteins, members of the nitrilase superfamily (putative class 13 nitrilases)
Uncharacterized subgroup of the nitrilase superfamily. This superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. Pyrococcus horikoshii Ph0642 is a hypothetical protein belonging to this subgroup. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). This subgroup was classified as belonging to class 13, which represents proteins that at the time were difficult to place in a distinct similarity group. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.
Statistics
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PSSM-Id: 143601
View PSSM: cd07577
Aligned: 14 rows
Threshold Bit Score: 409.381
Threshold Setting Gi: 124516252
Created: 28-Apr-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               #                                        
1J31_A         3 KVGYIQXEPKILELDKNYSKAEKLIKEASkegAKLVVLPELFDTGYNFESREEVFDVAQqi-----peGETTTFLXELAR 77
gi 170290570   3 RVGFVQTNPEFGAVESNLKRALDLASKVE---SDLLVFPELFNTGYLFLSREEALKLSEgl------dGPTIKRLSDFAS 73
gi 158340500   2 KVGFIQFSPILGKPTQTLERLEYLIDKGKh--SDILVLPELCNSGYAFNSRQQALNSSEsv-----adSKFIRFLHEKSN 74
gi 83816508    4 RAAYLQFAPAYLEVDQNLAAVESLLRSVE---ADLIVLPELFTSGYFFQSKDDLERVAEpi-----pnGKSVAALRGWAD 75
gi 206895228   2 KVGYVQFRPKFGDKEYNVSKMIDMASTLD---AEVIVFPELANTGYVFKSKEEIVTLAEpy------dGPSIKRMQEFSK 72
gi 218961272   2 KVSLLQFEPRLLDKEYNLKKMKQLLIGLK---TDLVVLPELCNSGYVFANKEEVFSVAEea-----gkGETYFVLNDIAN 73
gi 160903084   3 KVSVVQFKPELFEIEKNVNKVMKIIEGVD---SNFIVFPELAFTGYAFSSKKEVEETYEspl---dgiGYAFKTFKEFSK 76
gi 124516252   4 RIVLVQNNPVFGEVAGNLDRVKALYGGRKglrPDLVIFPELFASGYQFTSKSEALSLGEgdgrdgrekGPTVRFLEEFSM 83
gi 21673113    3 RLATVQFTPRLGERQANLEAIRSLLDPVE---ADIVVLPELCSSGYFFTSREELAPFAEsp------gGVACSFFQGLAD 73
gi 229535530   2 QVAYVQYNPAYLQITQNLKQVEALLEGIE---ADLIVLPELFASGYFFRSHEDLVAVAEpi-----peGPTTQWMIEWCR 73
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                            #   #  #                               ## ##
1J31_A        78 ELGLYIVAGTAEKSGnyLYNSAVVVGPRG-YIGKYRKIHLFYREKVFFEPGDl-GFKVFDIg-----fAKVGVXICFDWF 150
gi 170290570  74 EHSTAIVAGFPERDGgkVYNSAVAIDIDGdVKGVYRKTHLFYEEKLIFDPGDt-GFRVFDLa-----gMRVGIMICFDWV 147
gi 158340500  75 KYNLYIVSGFCEKSDkdLYNSSFLVGPNE-YIEVYRKSHLFLNEKDFFSPGNt-DYSVYDIg-----iCKIGMLICFDWV 147
gi 83816508   76 SLGATLVAGLAERDGdhFYNSAVVVRPDG-RVDTYRKVHLFYEETILFEAGDl-GFRVFEEhtaagtsYRLGVMVCFDWY 153
gi 206895228  73 RKGALLAFGFAERFGdvFYNSAAVVTPEG-EVKIYQKVHLFNEEKLYFNRGD--KFFTFTWh-----dTVFGVMICFDWI 144
gi 218961272  74 SINCNIVYGYPELDGnnLFNSCMLIMPDG-RFYNYRKTHLFNREKLFFSPGDt-GFQVFTIln----gVKIGMMICFDWQ 147
gi 160903084  77 DTGVSVVYGFNEKYEgkYYNSSILIKSDG-TYKIYRKTHLFFREKLFFTPGDt-GFWVDNIn-----gINVGVAICFDWY 149
gi 124516252  84 ETKGWVVGGLPLRRGnkVYNSAVVTHHGT-VMAIYDKTHLFEAENRWFERGSgpLCLVRTEf------GLMGVMICFDWL 156
gi 21673113   74 AKRAIIIAGMPETAQgcFYNSVFVFRPGVaDPLVYRKSHLFYKERFVFEPGDtgFPVIRDEql----dISIGIMLCYDWR 149
gi 229535530  74 KTGTVMVGGLPERDGdrFYNSAVAVGPEG-LIGRYRKVHLFYEEKLHFSPGNl-GFQVFEVadrngqlYRLGVMICFDWY 151
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                            # ##   #                                                    
1J31_A       151 FPESARTLALKGAEIIAHPANLVXP-YAPRAXPIRALENRVYTITADRVGee-----rgLKFIGKSLIASPKAEVLSIAS 224
gi 170290570 148 FPESARSLALSGAQIIAHPSCLVMP-YAPKADPVRAFENRVFIILSDRSGveer-ggkkLRYHGMSLISDPKMNILTQAP 225
gi 158340500 148 FPEAWRILALKGADIICHPSNIVLPdLCQKTIPGHSICNRMFIVTANRIGte-----dnLTFTGSSIICDPRGNILTQAS 222
gi 83816508  154 FPEAARTLALRGADVIAHPSNLVLP-HCPDSMPVRARENHVFTITANRHGreek-egesLRFIGMSEVCAPSGTILTRAD 231
gi 206895228 145 FPEAMRTLALMGAQIVLHPANLVLP-YCQDAMVTRCIENRVFEITANRIGve-----rgLKFTGASQITAPGGIVLHRAT 218
gi 218961272 148 FPEAARTLALLGAQIICHPANLVLP-WCQQAMKTRCLENRVFAVTANRIGtein-ageiFNFTGQSQILNTRGEVLSSLS 225
gi 160903084 150 FPESFRTLALLGADLILHPANLVLP-YCQEANKIRSLENKIYIATSNIWGtqin-geieYSFTGKSQVTSPDGEVLFRLP 227
gi 124516252 157 FPEVTRSLALSGALLIAHPVNWVLP-FGPQGMILRSVENRVFTATANRVGeearggfkpLRYIGSSQVVSPQGEILARAP 235
gi 21673113  150 FPEVSRVLALGGADLIACPSNLVTD-AWRKVMPARAIENKLYVAVANRCGtetr-gdetLLFKGCSAVYDPYGETVALAD 227
gi 229535530 152 FPEAARTLALQGADVIAHPSNLVRP-DCPRAMPIRALENHVFTITANRYGvesn-gretLRFIGRSLICDPAGRVLQEAP 229
                        250       260       270
                 ....*....|....*....|....*....|....*.
Feature 1                                            
1J31_A       225 eteeEIGVVEIDLNLARNKRLNDXNDIFKDRREEYY 260
gi 170290570 226 eegeHVGIAEIDPKLAEDKRVNELNDIFLDRRPEFY 261
gi 158340500 223 ddqeEVMISDIDLNLARDKSITSRNHIFLDRRPDKY 258
gi 83816508  232 esadVVEVVELNPREARDRNINAHNDVLRDRRPATY 267
gi 206895228 219 ktkeEALAVDIDPGLALQKSINSYNDLFKDRLPSAY 254
gi 218961272 226 vseeGTITVEINPEEANDKTVTERNDAFKDRRPEFY 261
gi 160903084 228 eqgdFIQTAEIDERKSQNKRINEYNDLFQDRKPKYY 263
gi 124516252 236 eeaeSLLEIQCDPELARSKRVVEESDFFRQRRPDLY 271
gi 21673113  228 adndRVLLAEIDPRSCRDKSFNEFNDIFADRRPELY 263
gi 229535530 230 pegdQVGIAEITPREARNRRITPHNDLFEDRRPDCY 265

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