Conserved Protein Domain Family
nitrilase_1_R1

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cd07578: nitrilase_1_R1 
First nitrilase domain of an uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases)
Members of this subgroup have two nitrilase domains. This is the first of those two domains. The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.
Statistics
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PSSM-Id: 143602
View PSSM: cd07578
Aligned: 4 rows
Threshold Bit Score: 459.69
Threshold Setting Gi: 221636203
Created: 1-Jun-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                #                                       
gi 39935483    5 LKVATVQFEPiMAEKERNIARLLELCEEAAvgGAKLIVTPEMGTTGYCWYDRAEVapfVEPIPGATTARFAELARKHDCY 84
gi 146309613   6 VKIAAIQFEPvMGDKARNIERLSALVEEAAkaGAELIVTPEMATTGYCWMSREEIaahVETVPGATSAHFAELARRYDCY 85
gi 221636203   5 YRAAAIQFEPeLGAKEQNVERLLSLCTAAAraGARLLVTPEMATTGYCWYDRREIapfVEPIPGPTTERFATLAREHHCF 84
gi 159186664   7 YTAAAIQFEPtMFEKARNISRLTALCEEAAqaGARLIVTPEMGTTGYCWFDRAEVkpfVETIPGPTTDVFHAIARQHRCY 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                        #   #  #                         ## ##          
gi 39935483   85 IVVGLPEVDE-DGIYYNSAVLIGPEGLIGRHRKTHPYiSEPKWSAAGDlhnQVFDTPIGRIALLICMDIHFVETARLMAL 163
gi 146309613  86 IVIGLPEVDDdSGLYYNSAFLVGPQGLVGTHRKTHPYiAEPKWAAPGDrghQVFDTPIGRIALLICMDIHFLETARVAGL 165
gi 221636203  85 IVLGLPEVDSrTGIFYNAAALIGPSGVLGVYRKTHSFiSEPKWAKDGDlgfPVFSTELGRLGILICMDADYFEPARLLAL 164
gi 159186664  87 IVVGMPEVDPeSDLYYNTAVLIGPDGVIGRHRKSHPYiAEPKWAANGDivhEVFETEIGRISMLVCMDLHFFETARLEAL 166
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  #                                                                     
gi 39935483  164 GGADIICHISNWlaertPAPYWISRAFENSCYVIESNRWGLERtVQFSGGSCVIAPdGSIAAVIDgGDGVAFAEIDLDtA 243
gi 146309613 166 GGADVICHLSNWlaeraPAPYWINRAYENGCYLIEGNRWGLERgVQFSGGSCIVEPdGSIQASRDsGDGVVLGEMDLQhA 245
gi 221636203 165 QAADVLCFPTNWllekgPGATWIARAFENGCYLIAADRYGCERgVQFSGGSAIIEPdGTIQARLDsGDGYLLGEIELDrA 244
gi 159186664 167 AGADIICHISNWlqertPAPYWINRAFENACYVIESNRWGLERtVQFSGGSCLIEPdGTVVASIDtGDGIAYGKVDLArA 246
                        250       260
                 ....*....|....*....|.
Feature 1                             
gi 39935483  244 RARQIGgea---vfRQRRPEL 261
gi 146309613 246 RQEQARr------mTLRRPER 260
gi 221636203 245 RHKRFPgslapeklTARRPEF 265
gi 159186664 247 RRREVLsep---vfKSRRPEL 264

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