Conserved Protein Domain Family
nitrilase_1_R2

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cd07579: nitrilase_1_R2 
Second nitrilase domain of an uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases)
Members of this subgroup have two nitrilase domains. This is the second of those two domains. The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.
Statistics
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PSSM-Id: 143603
View PSSM: cd07579
Aligned: 4 rows
Threshold Bit Score: 375.357
Threshold Setting Gi: 221636203
Created: 13-May-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                #                                       
gi 146309613 292 RVAVGQFTPaa-kQAGNLQRITELAVEAKae-gAELVVFPELALSGpyrgadma----eskdgagVSALLRLAQRLRLHL 365
gi 221636203 297 LIAVAQFLPkpgdLAANLATIDRSLAALPr--gTRLAVFPEYAATGvphdaseatafaasdtaslLRALRRLARRHRTAL 374
gi 39935483  293 RLTAAQFAPtd-dIGGNLAQIDALARQAKan-gAEMVVFPELSLTGlddparta----vavpgpaTDRLAALASELSLYL 366
gi 159186664 296 RAAVAQFAPts-vIADNIAHITRLATEAKattaPDILVFPELSLTGlddphtra----eplsgpaVSVFVRLAMKLGLYL 370
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                     #   #  #                         ## ##             
gi 146309613 366 VaGFVERdgealYNSAVLVGPEGvvGCYRKIHLNeQERAWATAGEc-wRHYDLpfgRIGLLIGEDCLVPEaGRVLAlrgC 444
gi 221636203 375 VvGFLEAlpggfASSAALVTPSGltVTYRKTHVIgPERSFLVPGDtppPVIDLplgRLGLLIGSDLCFPEiARVLAlagC 454
gi 39935483  367 VcGLAERdgdilYNSAVLIAPDGtiTTYRKTHLTeNERGWAQPGDs-fVVCDTplgRVGLLIGHDAIFPEaGRVLAlrgC 445
gi 159186664 371 VaGFAEAdgdriYNSAVLAGPEGlvGNYRKTHLG-VADSWATAGDd-wKIYDLaigRVGLAIGHDALYPEaIRSLSlmgC 448
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #                                                                        
gi 146309613 445 DLLACSTAlaeplpgahagsvirqnyPIPMGADPyHWHLGRVRAGENNVYLAFANsfdtdsgacGGSGVFGAd------- 517
gi 221636203 455 DLLAVPAGpgippvqalgptsvplppPAVTGDDPtHFHLARVRACENMTVVAYAAlplp--egtGWSGLFGPvpesrase 532
gi 39935483  446 DIIACPAAietrfstphagtsvkqpaPIPTGADPhHWHHFRVRAGENNVFFAFANvadiargypGLSGVFGPd------- 518
gi 159186664 449 DLVACPSAiagnftgshagtkiphnyPIPKGADPfHWHALRVRGGENNVYFAFANvldaargyqGKSAVFGPd------- 521
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|...
Feature 1                                                             
gi 146309613 518 TFAFPRHEaiigdasGVAVASIDTSNLDARYPTNVVRRKDLVLMRQPLQYGAL 570
gi 221636203 533 RLVEPGQA-------GLSWGLLDTRNAASRYPTNPLRAKDMLRMRQPYWYAPL 578
gi 39935483  519 TFEFPRREalmgseeGIATATIDTSNLDSVYPTNVVRRKDLVAMRMPHSYRPL 571
gi 159186664 522 SFAFPRQEsaildeeGIAAAAVDTTNLDTPYPTNIVRRKDLVVMRQPHHYQPL 574

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