Conserved Protein Domain Family
nitrilase_2

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cd07580: nitrilase_2 
Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases)
The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.
Statistics
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PSSM-Id: 143604
View PSSM: cd07580
Aligned: 13 rows
Threshold Bit Score: 347.411
Threshold Setting Gi: 118468846
Created: 8-May-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                 #                                      
gi 170750810  13 RVSCIQFEpef--gaVDANMARASDLVRAAAaegSRLIVLPELASTGYVFesaaeaaalaepvpdGPTTRAWAALAAEL- 89
gi 33600531    6 KIAAIQFDvrq--geNDANRARSCELARQAAaagANLIVLPECCVGGLVFdsrdeir-avsetvpGPSTRAWSQVSRET- 81
gi 118468846   6 RVSAVTPDivi--gdLDGNLARLHNALRAIEwtgPYLAVLPELATSGYVFtdidea----ralslRADDARLTALADDVp 79
gi 88854385    7 TVASCQVAlav--gdLAANRRSLRSAISEAAdagAQVVVLPELANTGYMFadigelr-slaepvdGLTVSEWVSLAGEH- 82
gi 15597270    5 TVACCQIApri--gaQEHNLRLAERAIREAArrgANVVVLPELAASGYVFadrgeal-alaetrdGPSLGLWKALAGEL- 80
gi 224822834  30 RIAVAQTTpaf--geIERNREHTVELVREHAd--ADLLVLPELATSGYVFesesela-tmaeprdGATADAWADVAAET- 103
gi 118471653  13 RVAVVQFNpqvgvenLKANSEAVYERLQQAVaggANLIVLPELATTGYTFesreeayahaepvpsGATVTGWAEFAAAH- 91
gi 229361535   9 RVAVIQLDpqvgmnnRANNLHQSLALATEAAnggANLIVLPELTNCGYFFssrqdafdhaelvpdGTSVQAWIDFACRH- 87
gi 90417955    8 QISCAQFEpri--geVERNVEASLKLIAEAAdsgSRLIVLPELCNSGYVLesreeayalsedvatGSSIARWASLAAER- 84
gi 221215298  10 KIACLQFSplf--gdVAGNLQRSIAMIEKAAdagARIVVLPELANTGYTFksraeafelaepvpdGRSTQAWAEVAACR- 86
gi 187922083   2 QIACVQMApri--geKDLNVRRAIDFVTRAAdlgAELVVLPELANTGYVFdsraeaeslaepvpdGSTTAAWSEVASAL- 78
gi 91781311   15 VIACIQMEphv--gaKRDNVTRSIRHIETAAkngASLVILPELANTGYVFvdrdeafslaeelpdGQTARIFAEMARRL- 91
gi 209546079  12 TVATVQFEpiv--gdRAHNLAAIERLARAAKakgAEIVVLPELADSGYSFhdgdevaalagpvpgGLSAETLCRLAREL- 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                   #   #   #                         ## 
gi 170750810  90 -GVHIVAGIAESAGe---------ILYNAAVIVGPGGYIGTYRKAHLWd-QENVFFARGDlgfPVFDTalgKVGVAICYD 158
gi 33600531   82 -GAWIVAGLSETDGa---------KIYNTAVLVGPNGELHRHRKLHVRg-IEQRLFDVGDa-lTCVDTplgRIGLAICYD 149
gi 118468846  80 aDCVAVVGFAEVDGd---------TLYNSAIVIGDGKVVGTYRKAHLWa-AEPEIFATGVeagTVIDTaicRLGVAICYD 149
gi 88854385   83 -NLIIVGGFAEAGAd--------cEVFNSAVIVDASGVLACYRKAHLWntEKADLFTPGSgtpPVVDTavgRIGVMVCYD 153
gi 15597270   81 -DLVIVGGFCERLDp--------qRVANSAALVDADGVRAIYRKAHLWn-EESGIFEAGEqppPVVATrfgRIAVMVCYD 150
gi 224822834 104 -DTWIVGGFPEAADvdtdttdansRYYNSALVVSPNGVEAVYRKLHLWn-EEHRWFTAGDe-lPVVGTpfgRLGVQICND 180
gi 118471653  92 -DVYIVGCLPELDGv---------ELFDTAVLVGPEGYIGKYRKTHLWn-EEKLFFSPGDlgyPVFHTrigRIGLLVCWD 160
gi 229361535  88 -QVYLVAGLCEIDGm---------QLFNTAVLLGPDGFIGKYRKAHLWn-LEKLWFTPGNtgfPVFETpigRIGLLICWD 156
gi 90417955   85 -GLYIVAGFLERDGi---------KLYNSAIVIGPDGVIGTYRKNHLWa-DEALYFERGDlgfPVFHTpfgRVGVLICYD 153
gi 221215298  87 -NLIIVAGITEREDn---------CLYNSAVVLDPSGTLGTYRKLHLWg-DENLYFERGNlgvPVFATpygRIGVAICYD 155
gi 187922083  79 -GVYIVAGIAEKADg---------ALYNSAVLLGPDGYLGSYRKTHLWg-DEKKLFASGDrpsPIYDTplgRIAIAICYD 147
gi 91781311   92 -GIHIVAGIAERAGt---------RLYNSAIFTGPSGHIGVYRKLHLWn-NERLFFEPGDfgiPVFDTsvgRIAIVICYD 160
gi 209546079  89 -CLYIVSGVAEQDGd---------RFYNSALLCGPDGYIGKYRKLHLWn-NENRLFRKGDiglPVFDLpfgRIGIAICYD 157
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ##                    #                                                         
gi 170750810 159 GWFPEtFRRLAlaGAEIVCIPTNWvpmpdqp----egeaamANTLHRAAAHTNGIFIACADRVGIERGqpFEGQSLIIGP 234
gi 33600531  150 MWFPEvCRNYAldGVDVVAAPANWsksvrtadafdayglpqGYHLMAATAVSNELVVVAADRVGTERGvaFLGTSCIFGP 229
gi 118468846 150 NEFPElPRRLAlrGAEVLALPVNWplvdrp-----egehapETIQAMAAARSSQLATVIADRRGTERGvrWTGGTAVIGP 224
gi 88854385  154 LEFPEwVRTAAleGAELLCCPVNWplypap-----egerpiEVIKVQAAAATNRMFVAAADRAGIERGqdWLGGSVIVDA 228
gi 15597270  151 LEFPEwVRLPAlsGAQLLCAPVNWpaaprp-----egerpaEVVRVQANASVNRLFIAACDRCGQERGvdWVGGSVIVDA 225
gi 224822834 181 LWFPElTVTQAkaGVDLVAVPTNWvpgpadgt--rpggwtiGAHQALAHANANRVFVACADRAGTERGvtFEGQSVILDP 258
gi 118471653 161 IWFPEtARIVAqqGADIICIPTGWvwtppply--ddsgtcmAAYLTMTAAHVNNVFIATADRIGTERTsgFMGNSLIAGT 238
gi 229361535 157 IWFPEvPRILGqqGADIICSLNNWvwtppplf--deagkcmASYLTMTAAHVNNVFIAAASRIGEERGarYLGCSLIAGT 234
gi 90417955  154 GWFPEaWRILAlqGADIVCVPTNWvpmaeqp----agmpamANVLCMGAAHSNSMVVAACDRVGTERGqpFIGQSLIVDH 229
gi 221215298 156 GWFPEvFRLAAvqGADLMCVPTNWvpipgqq----pdqppmANILHMALAHSNSMVIACADRVGVERGqpFIGHSLIVGH 231
gi 187922083 148 LWFPElFRLYAlgGAQIVCVPTNWvpmpnqs----adrpamANTLAMAAAHTNGLVVACADRVGVERGqpFEGQSLVVDQ 223
gi 91781311  161 GWFPEtYRLAAmqGADIVCVPTNWvpmssqp----ddrpamATTLTMAAAHSNGMAIACANRIGTERGqpFIGQSVIVGG 236
gi 209546079 158 GWFPEtFRELAlaGAELVCVPTNWvpmagae----siaepmANILHKAAAHTNGLYIACADRIGVERGqsFIGRSLIVGP 233
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*....
Feature 1                                                         
gi 170750810 235 kGWPLAGPAsrdrtETLSALVDLD--AAGRKDlnrf-nsllRDRRTDIY 280
gi 33600531  230 sGEALCPAAsrgeeQILLADWPDI--AAVRAQggp----hlGQRQPAVY 272
gi 118468846 225 dGWIKATPQgd--eTIATAVLDLTdsKAIGDRnd-----alADRRPDLY 266
gi 88854385  229 dGFPLTTLDlg-vaSVHIAKLDLA--DARNKTisel-ndvhADRRPELY 273
gi 15597270  226 dGYPLDGPLqg-eaGMLLARLDLA--EADEKRisah-nhvhRDRRPELY 270
gi 224822834 259 nGLPLAGPApvdgeHALTTACDLT--RARNKEltpy-ddalASRRPEHY 304
gi 118471653 239 nGWPIDRIAgpdedTILYADIDIT--ASRGAPiwnqfndlhRDRRTDLY 285
gi 229361535 235 nGWPIGAVAsanrqEILFADIDVT--SARSAPiwnslndlqRDRRNDLY 281
gi 90417955  230 nGWPVAGPAsasepGLVTAICDLS--QARRKRnwndfnqvmRDRRTDLY 276
gi 221215298 232 tGWPVAGPAsgsdeEILIGEVSLS--AARRDRnwnsfnhplHDRRTDFY 278
gi 187922083 224 sGWPVGDVAsrsleEIVSASITLD--ARTSVGthn---dvlADRRADIY 267
gi 91781311  237 dGWPITGPAsgdreEILYASIDLK--RTRSSRalnalnhvlHDRRADVY 283
gi 209546079 234 qGWPISGPAsadreEILLAQIDLS--SVTETRtlnsfnhllGDRRADVY 280

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