Conserved Protein Domain Family
nitrilase_3

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cd07581: nitrilase_3 
Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases)
The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.
Statistics
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PSSM-Id: 143605
View PSSM: cd07581
Aligned: 52 rows
Threshold Bit Score: 277.535
Threshold Setting Gi: 124027487
Created: 8-May-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               #                                        
gi 121609788   3 VALGQFAvrpeWQRNADTCLELMAQAGre--rADLLVLPEGVLArditdpdl---vrrsaqpLDGPFVTRLLQASRSApl 77
gi 124028507   6 IALLQFGathsKEESLERIRKLISRYErivseADLLLVPEYSMAdptgqppe--aiaaiaepLEGPWIGFFARLAREY-- 81
gi 167747387   5 AAIAQCRasqePEKNLCKAEFFCREARee--sACFVIFPEYFMTyypiegsa---yyekaqtLDGPFVQGMKRLAAAH-- 77
gi 229879085   5 IALAQSGfp-kKGDAIAQAERYAEKAAaa--gAQLLVFPENFMLpgildseg---vkeisepLDGPFARAMADIALRH-- 76
gi 167768350   4 IAVAQTTssgnWRENIEKAKQYIKKATee--eAVMIIFPEYFMNyypdaehn---ytkkaqsLHGEFVQAMKMLAKEF-- 76
gi 119720138  18 VALHQLAvsgeKRENLEKALRLLELGD-----AYLHVFPEYLMGvdpggptrd-yvwrvaepIDGEFASRIVEKTGEL-- 89
gi 118431809   3 IAVLQVAstreKDANLESVKRLASRVKn---sPDIVLTPEYLMLdptglgrd--aiydaaedLEGRWSRELSKIAESL-- 75
gi 119873341   1 MFRLGIAq--iSPGNLDKLRSLVGRSE-----PDLLLLPEYSMFdptglppe---eiwnkavAIEDFTSLIGRLAREL-- 68
gi 218884032   6 IGIVQAGfndlPAENAGKAIDIVRKNYk---eADIVVLPEYSMLnplkigdprlvysysetpLTSRYLTEMAKLAGEL-- 80
gi 126465126   6 VGILQDEftvdPMENYKRIRELLEEKHq---vADIVVVPEYSMInilaglkpsdvyeraeriNDSIYISKISDLAAKL-- 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               #   #      #                            #
gi 121609788  78 tGLTVMm-CVHVPaan--dkvFNLLIAL-RDGQiLAQYRKLHLYDAFsAQESANVMPGDev-pPLIEVag--lKLGLMTC 150
gi 124028507  82 -SVHVVatLYEKSkag--gkpYNTAALIaPTGElLAVYRKIHLFDAYgYRESDYFMPGAe---PAKLAtikgfRIALAVC 155
gi 167747387  78 -GQWILfgMSEKPgrdsggkcFNTAVLLdDRGNlCSVYRKTHLFDAFsWKESDSTLPGEvlhePVNTPl---gKAGLGTC 153
gi 229879085  77 -HLWIVyvIFEENpdg--gqpFNTAVTVdAEGVqRGAYRKCHLYDAHgVLESDRSVAGDqlpqPVDTPf---gPLGVAIC 150
gi 167768350  77 -KMWIIfgMNEQTvde--tknYNTMVILnDLGElVSDYKKTHLFNAYkWNESMNTLKGDclftPIKTPa---gVIGLGIC 150
gi 119720138  90 -GVAAVftMFLREgp----gvYNAAVLA-EEGKvKAVYRKIHLFDAYgYRESSVFSPGRe---PVVADlk-glRLGIAVC 159
gi 118431809  76 -GSCLLghLFLKTpsg---rvANAAVLYsRDGGiIGVYRKTHLFDAYgYVESSFTEPGDelwePRKACg---aSIGVAIC 148
gi 119873341  69 -GAYVVggFLERGprp---kvYNTTVLVdPGGRvVGLYRKTHLFDAYgYKESEFVEPGAel-sQVYTVrg--aRVAFAVC 141
gi 218884032  81 -GVFILvhFIEKTdtp--pltMSTSIIVtDRGEaIPVYSKMHLFDAYgFRESSFFKPGNv---PGKIIsikgvKIGFTIC 154
gi 126465126  81 -DTYMLihFIEKTdtp--pktMSSSILVhPSGRiDKVYSKMHLFDAYgYRESDYFLPGRtlsrPLVFNh---vRFYVAIC 154
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        # ##                     #                                                      
gi 121609788 151 YDLRFPElARRLAl-dGAEVLVLPAAWVKGPLKeahWELLLSARALENTCYAVAAGecge-rniGHSMVVDPlGVVVARA 228
gi 124028507 156 FDLRFPElFRTYAl-qGAELVAVPAAWYRGPAKedqLRIIAAARAHENTMYIAVASqyns-nftGRSLVADPyGLVLLDA 233
gi 167747387 154 YDLRFPEpARIQAl-kGAEVLIYPSAWVDGPDKfmqWEALLRARAIENTVFTIGCChyseehymGRSLAFDPsGRKLAEG 232
gi 229879085 151 YDLRFPEvARALAv-aGCKLVLFPSAWNAGPRKvkhWTTLLRARAIENELFVAGIChagk-rytGKTLVADPlGRIVASA 228
gi 167768350 151 YDLRFPElARYEAl-sGAQVMLYPAAWVKGKGKfmqWETLLRARAIENEMYVLGCChyskehymGRSTGFAP-DGTRLYL 228
gi 119720138 160 FDLRFPElFRSMFl-rGAEVFVVPSAWYRGPYKveqWKALTAARAHENTSYLVAVNqvge-sftGHSLVATPlGHLLVDL 237
gi 118431809 149 YELRFPEiFRTQSlvgGVDIFLVPAAWYRGPGKeeaLSVLSRARAQENTSYVAVASnaga-nfvGRSMIIHPlGYTLAQA 227
gi 119873341 142 FELRFPEvFRELAl-aGADLVAVPAAWYAGPLKeeiLHILARARAIENGMYIAVSAlwgp-rftGRSVVVNPfGVVEAEL 219
gi 218884032 155 YDLRFPElYRITAl-mGSEIIIVQAGWVKGPHKeeiLDKLASIRAHENTVYIVLADqtgd-mfvGRSGVFNPwGYRELDL 232
gi 126465126 155 YDLRFPElFRSYAr-kDAYGVFIHAGWVRGPLKeeiLDLLARARSHENTMYIILSDqtgk-qyvGRSGVFSPhGFREIDM 232
                        250       260       270
                 ....*....|....*....|....*....|....*.
Feature 1                                            
gi 121609788 229 Aes------pALLIAALDPARIAHARrvLPVLANRR 258
gi 124028507 234 Grg------eKIVQIEVDRESLEEARrqLPLLKLRR 263
gi 167747387 233 Eag------eELIVLPVDTDQRDAIIhqVPVMKNRR 262
gi 229879085 229 RgsskgnapqDLVICTIDTDAVESARdaMPVLDHRR 264
gi 167768350 229 Gee-----keELLYAQIDKEQIQNIRtaNPVFENRR 259
gi 119720138 238 Geg------eRSIAVDLDEGELASARerIPVRDLLR 267
gi 118431809 228 Ppw------eWVLEHEIDLREIERARksLPVLEHVK 257
gi 119873341 220 Gag------eRYREVLIDLRLVEEARktVPTIYLRR 249
gi 218884032 233 Gwr------eAYVEYTIDASLISEVRekLPVLQQSK 262
gi 126465126 233 Gyr------rGYVEWPINPDDVLEARkvVPVVEQSR 262

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