Conserved Protein Domain Family
nitrilase_4

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cd07582: nitrilase_4 
Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases)
The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.
Statistics
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PSSM-Id: 143606
View PSSM: cd07582
Aligned: 10 rows
Threshold Bit Score: 376.684
Threshold Setting Gi: 182414759
Created: 8-May-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                  #                     
gi 116670475   5 FVVVAVSPRtin-------vknPGDGVANVKRINEFIDTAVMVgawe-gspVKLVVLPEMAIQgmmantpgnrkk--eah 74
gi 114799416  34 YTALALQTVceavnqdktaeasHARMNAAITRVTGQIMASRGFlkgfngydLKLVVLPEYWMTgfpmgetretwq---ak 110
gi 182414759   3 YAVAACQTDfac-------ptdRREISARVDRMLAMIDGAVVGyrp--ffpVRLVVFPEFAHAapiyatvrelr----dk 69
gi 89893122    5 YMALAIQPTmrg-------aekREDIKRNIAHISDVIDAAVWLsgi--empVRLITIPEGALQgftdelfdwdhqyyvdh 75
gi 212712633   5 YGVVGLVPTtwg-------iktRSDIMKNIDHLEELSHGALWLssl--dlpVRLLVLPEGALQgfndealdlpiaeflkd 75
gi 156743444  29 YRALALQVTchavnalnnrvavREQMLATIVRLREQIRASIAFig----ndVRLVVLPEYFLTgfplgesiavws---ek 101
gi 17232515   11 FRALALQVTchavnqasdrqeaGLMMHNTINRLAQQIAASIAFig----fdCRLIVLPEYFLTgfpmgeplqvwa---dk 83
gi 148554614   9 YTALVMQPHvev-------aedRAGIAKNLDRAINMIDFGVGYfwe---lpARLVVFPEYFMQgvttpgkgehgidsfmk 78
gi 109897826   5 YTAVALQTRcfavnk-lnvadaRQKIFDNIRRVGRHVQGTKGFvg----psVKMVVLPEYFLTsfpmgeslaqwk---ek 76
gi 227818366   5 YTAVALSTRnya-------vktTKESLENIKRINDFMDVAVMVaatd-gapVRLIVLPEMAIQgmpmdfragnkeq-hes 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                   #   #                
gi 116670475  75 favtipgpeTDELAKKAVELNTYIAaELY-MVkDEDFPDRHFNVAFIIDPQGeIIYKRYKATSDayeggmlgnmnPHDVW 153
gi 114799416 111 aaipmdgafPDALAKMAQTAGVYLCsNHY-ET-DPNFPELYFQANVIYGPTGdTLLRYRRMISLys-------psPYDVW 181
gi 182414759  70 lavpvpnehTARYHAKARELGIYIQtGTFiES-DPAHPGVLFNTSCLIGPEG-ILTKYRKVNPWi----------PWEVH 137
gi 89893122   76 maidipgeeTELLGKKAKEYNAYLIaQAK-VK-HPEFPGRFFNSAFLIDPKGeVILQSYKMQVFcqeh----stvPHDVW 149
gi 212712633  76 gaidipgpeTDRLGELAKSQNVYIMaQAK-TR-DPDWPGIMFNVGFIIDPKGnIILKHHKMSSLlpce---rsasPHDLY 150
gi 156743444 102 aaidpdgpeYAALGQIACDLQIFLAgNCY-ER-DPHFPGLYFQVSFVIDPSGqCVLRYRRLNSMfa-------ptPHDVW 172
gi 17232515   84 acleingaeYEALGMIAQKQQIFLAgNAY-EL-DPNFPGLYFQTCFVIDPSGsVVLRYRRLNSLfa-------ptPHDVW 154
gi 148554614  79 kaieldgpeMRRLGEVAKEYNLYIAgGGVvER-VKEFPDRWFNTAFIIGPSGeVVLRYHKWHIPasig---lgtsPHDIF 154
gi 109897826  77 gaiaqdgkeYELLSEIAQKNQVYLSgNVY-EL-DPHFKDLYFQTSFILGPNGdCILRYRRLVSMfa-------ptPHDVL 147
gi 227818366  76 aattipgpeTEALAVKAKQFNAYIAaELLwVR-DDDFPGHLFNVGFIINPQGeIIYRRAKATSDayeggalgttnPHDIW 154
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #                              ## ##                    #                       
gi 116670475 154 DEWIEKKGNgnamDAIFPVAKTEIGNIGYAICHEGVYPEvPRGLAmnGAEIIIRGTLiepav----qngmWELQNRAHAM 229
gi 114799416 182 DKYLDIYGA----DAIFPVAETEIGALATIASEEILYPEiARMHAfkGAEILLHPTSevgsp----sltpKHIAKKARAI 253
gi 182414759 138 ASPHDVPGYa---EELFPVAQTELGNLGCAICYDWIFPEaIRALTlnGAEVLIRVSAymdpwgatppmdwWTVVNRCRAL 214
gi 89893122  150 DKWIELYGYk--lDSFYSVADTEIGRIGLLVCQEGDYPEpARGLAmnGAEIIYRSSApepav----angwWELQNRARAL 223
gi 212712633 151 DEWIKKYGRs--lQSFWPVVDTEIGRLGIMMAMEGNFPEnGRGLAlnGAEVVYRASLptpft----endlFEISNRARAL 224
gi 156743444 173 DRFCQMYGP----DALFPVADTAIGRLACIASEEILFPEvARCLAmrGAEVFLHSSSevssp----eltpKHIAKRARAL 244
gi 17232515  155 DKYLDCYGL----EGVFPVAKTAIGNLAALASEEILYPEvARCLAmrGAEIFLHSTSevysk----nltpKDAAKITRAV 226
gi 148554614 155 DEYKEVFGGd--iSTLFPVIDTEIGKLGTMTCHDGCTPEvSRALGynGVEVICHPVAlqevegvsqpwdfWMFTRRTRAH 232
gi 109897826 148 DKYLDVYGQ----SSLFPVAKTELGNLACVASEEILYPEiSRCLVmnGAEVLLHSSSevgsv----eltpKDIAKRARAV 219
gi 227818366 155 EEWVEKKGNgsklDAFFPVARTEIGNIGYLICHEGAYPEtARGLAmnGCEIMIRATLiepqv----mqgmWELQNRAHAM 230
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 116670475 230 FNSAYIVAPNlgpevrdd--gsmqdlfgGQSMIVGPrGQILTKQQgwtsgdSFVCTTIDIEALRRARvanglynqfKDLR 307
gi 114799416 254 ENISYVVSANsasitgtp---vpaastdGMSIVLDWyGRTLAEAGtg--etMNANAVLDLTALRETRrktgmtntlSRLP 328
gi 182414759 215 ENLAYVVAANqgaslqh----ygpfswpGGSMVVDYdGRILAQADpgp-geKIVVAEIDLDALRAARarrlghnplAHLR 289
gi 89893122  224 DNTCYVVAPNvatyypsqaaelsvdtfgGQSMIVNHhGEVISNHKygs-gsSYAGAIIDVESLREYRerslfgnwmKDLR 302
gi 212712633 225 ENNFYVVAPNigsmymfpttqapidcggGRSLIVDYkGKIVGKQEdtn-gsTYVYGVIDIEAMRYHRanasvsnwlKDIR 303
gi 156743444 245 ENLAYVISANsagisgip---ipaasveGGSQIVDYtGRVLVEAGqg--esMAAHAEIDLAALRRYRrrpgmnnllSRQR 319
gi 17232515  227 ENMAYVVSANsaglansa---ipigsvdGGSKIIDHrGIILAETGpg--esMAAFAEIDLAALRRDRtrpglnnllSRQR 301
gi 148554614 233 DNMAYVLGSNwgtvdyay---ypkafcpGGSFAIDYtGMVQRHAPyp--qeQVLGVTIDIESLREHRsrcn-hntwVDVR 306
gi 109897826 220 ENLAYVVSANsagiadid---fpaestdGMSKLVDFkGRVMAEAGmg--esMVANAELDITALRRYRnkpgmgnilSRQR 294
gi 227818366 231 FNQMMVISPNlgpqvgkd--ggffdvfgGQSMIVDHkGHVLVKQPgmangdSFVSTVVDVEALRRARiangvtnwfKDLR 308

                 .
Feature 1         
gi 116670475 308 T 308
gi 114799416 329 L 329
gi 182414759 290 R 290
gi 89893122  303 T 303
gi 212712633 304 T 304
gi 156743444 320 F 320
gi 17232515  302 F 302
gi 148554614 307 T 307
gi 109897826 295 N 295
gi 227818366 309 T 309

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