2VHI,2VHI


Conserved Protein Domain Family
ML_beta-AS

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cd07587: ML_beta-AS 
Click on image for an interactive view with Cn3D
mammalian-like beta-alanine synthase (beta-AS) and similar proteins (class 5 nitrilases)
This subgroup includes mammalian-like beta-AS (EC 3.5.1.6, also known as beta-ureidopropionase or N-carbamoyl-beta-alanine amidohydrolase). This enzyme catalyzes the third and final step in the catabolic pyrimidine catabolic pathway responsible for the degradation of uracil and thymine, the hydrolysis of N-carbamyl-beta-alanine and N-carbamyl-beta-aminoisobutyrate to the beta-amino acids, beta-alanine and beta-aminoisobutyrate respectively. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 5. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer. Beta-ASs from this subgroup are found in various oligomeric states, dimer (human), hexamer (calf liver), decamer (Arabidopsis and Zea mays), and in the case of Drosophila melanogaster beta-AS, as a homooctamer assembled as a left-handed helical turn, with the possibility of higher order oligomers formed by adding dimers at either end. Rat beta-AS changes its oligomeric state (hexamer, trimer, dodecamer) in response to allosteric effectors. Eukaryotic Saccharomyces kluyveri beta-AS belongs to a different superfamily.
Statistics
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PSSM-Id: 143611
View PSSM: cd07587
Aligned: 18 rows
Threshold Bit Score: 749.194
Threshold Setting Gi: 30698009
Created: 21-Apr-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
2VHI_C          9 LNDCLEKHLPPDELKEVKRILYg---vEEDQTLELPTSAKDIAEQNGFDIKGYRFTAREeqtrKRRIVRVGAIQNSIVIP 85
gi 169158995    9 LEKVLEAHLPEAELKEARRLLFg----KELKKLDLPRSAIDAAGQQDFDLKGYVFEASPeqlrPPRMVRVGLIQNKIVLP 84
gi 215504604   10 VEKILKEHLPAEEHEKVRAVLYg----RDCSPLEFSSDALGQAKQHKFELKGYKMAAEReelrPARVVRVGLVQNKIVLP 85
gi 196006812    8 LESLLEKNLQGRDLDEAKRILYg----SGFSALTLPETASREATDLDFELKGYKISAQAeetrPPRIVRIGLVQNKIHLP 83
gi 74866565     8 VQATLEKYIPAEELSEVKRILYgynrgHHVKSLPICQEALDLANKNNFEIVASKVEADPeqlrKPRIVRLGIIQNSIGAE 87
gi 148907795   43 LHDLLQSNLPPSLYKEVSQLLLgvncgKVVSPLSLPETVLARSKESSFDLQAYRFNAESemmrAPRIVRVGLIQNSIVLP 122
gi 30698009    23 LHQLLSANLKPELYQEVNRLLLgrncgRSLEQIVLPESAKALSSKHDFDLQAASFSADKeqmrNPRVVRVGLIQNSIALP 102
gi 168030450   20 LHRLLSSELPPHLFQEVSRVLYgcnrgSAVTPVPQTEDLSAAAKEGNFDVQTFEFTADLeqmrAPRNVCVGLIQNAIVLP 99
gi 118361640  623 VEQAFDTYIPEKVRSEVYRILYg----QNYKPLDLALRTHEMALEHNFEIKGFRVQANKeqtrPPRLVRIGAVQNIYKAS 698
gi 219481988    8 VDDVLREHIPPEKLGEVQRVLYg----SSFSKLNLPAEAVKLANERKFELAGYGMQAVPeqlrPARLVRVGAVQNKIVLP 83
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                           #                                             
2VHI_C         86 TTaPIEKQREAIWNKVKTMIKAAAeAGCNIVCTQEAWTMPFAFCTREKfPWCEFAEEaenGPTTKMLAELAKAYNMVIIH 165
gi 169158995   85 TDaPVLEQITALHKRVGEMVEVAAmCGVNIVCFQEAWTMPFAFCTRERePWTEFAESaedGLTTRFCIQLAKKHNMVVVS 164
gi 215504604   86 TTeTVTAQREALHKRIETIVDTAAlCGVNIICFQETWHMPFAFCTREKtPWCEFAESaehGPTVQLCQQMAKKHNMVVVC 165
gi 196006812   84 TTaSIVEQREALHQRISQITKVAAdCGVNVICYQEAWNMPFAFCTREKhPWCQFAESaedGPSAKLCCQLAERYNMVILS 163
gi 74866565    88 TTaPIQDQYLAIEAKIEKMIDAAGaMGVNVLCLQETWHMPFAFCTREKyPWVEFAESastGQSIKFIQRMARKYNMVIIS 167
gi 148907795  123 TTaPFSEQRKAIMQKVKCMIEAAGvAGVNIVCLQEAWTMPFAFCTREK-RWCEFAESv-dGESTQFLKELAQKYNMVIIS 200
gi 30698009   103 TTaPFSDQTRGIFDKLKPIIDAAGvAGVNILCLQEAWTMPFAFCTRER-RWCEFAEPv-dGESTKFLQELAKKYNMVIVS 180
gi 168030450  100 TTePFLKQKHAIMERVTSLIESAAkAGVNILCLQEAWTMPFGFCTREK-RWCEFAESaeaGESTKLLQGLAQRYNMVIVS 178
gi 118361640  699 PSqPIQEQRQAIHDYNKKVVEAAYhSGVNVICFQELWTCPFFVATREKyPWIELAESaehGPTTQMLQELARKYNMVIVN 778
gi 219481988   84 TSaPIAEQVAALHSRIAEIVEVAAmCGVNIICFQETWTMPFAFCTREKqPWCEFSESaedGPTITFCQELAKKYNMVIIS 163
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                        #   #      #                         ## ##       
2VHI_C        166 SILERDmehgETIWNTAVVISNSGRYLGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETeFGKLAVNICYGRHHPQNWMM 245
gi 169158995  165 PILERDeihgGTLWNTAVVVSNNGNVLGKTRKNHIPRVGDFNESTYYMEGNTGHRVFQTqFGKIAVNICYGRHHPLNWLM 244
gi 215504604  166 PILERDe--nDVMWNTAVVVSNSGAILGKSRKNHIPRVGDFNESTYYMESKLGHPVFQTqFGKIAINICYGRHHPLNWFM 243
gi 196006812  164 PILERDslhgDTIFNTTTVISNHGKVIGKTRKNHIPRVGAFNESTYYMEGDTGHRVFETdFGKIAINICYGRHHPLNWLM 243
gi 74866565   168 PMLERDdvhaSTIHNTAVVVGNNGNIIGKSRKNHIPRTGDFNESTYYMESTLGHPVFETiYGKIAINICYGRHHNLNWLA 247
gi 148907795  201 PILERDmthgEVIWNTAIVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETcYGKIGINICYGRHHPLNWLA 280
gi 30698009   181 PILERDidhgEVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETvFGKIAVNICYGRHHPLNWLA 260
gi 168030450  179 PILERDeshgSTIWNTAVVIGNHGNVIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETeFGKIAVNICYGRHHPMNWQA 258
gi 118361640  779 SILERDde-rGTIYNTTVVISSKGKFLGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETeFGKIGVNICYDRHHPLSWQQ 857
gi 219481988  164 PILERDaahgDIIANTAVVISNTGTVLGKSRKNHIPRVGDFNESTYYMEGNTGHRVFQTqFGKIAINICFGRHHPLNWMM 243
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                      #                                                                  
2VHI_C        246 FGLNGAEIVFNPSATIGrLSEPLWSIEARNAAIANSYFTVPINRVGTEQFPNEYTSGDGNKAHKEFGPFYGSSYVAAPDG 325
gi 169158995  245 YSVNGAEIIFNPSATVGlLSEPMWPIEARNAAIANHCFTCAINRVGTEYFKNEFTSGDGKKAHHDFGHFYGSSYMAAPDG 324
gi 215504604  244 YAANGADIVFNPSATVAgLSEPLWHVEARNAAIANSYFTCAINRVGTEVFPNEFTSGNKKGAHKDFGHFYGSSYVAAPDG 323
gi 196006812  244 YGVNGAEIVFNPSATVGaLSEPMWSIEARNAAIANTYFTAGINRVGTESYPNEFTDGDGKPAHHDFGHFYGSSYVAAPNG 323
gi 74866565   248 YGLNGAEIVFNPSATVGeLSEPMWGVEARNAAMTNNYFVGSINRVGTEHFPNEFTSGNGKPAHKDFGHFYGSSYFSSPDN 327
gi 148907795  281 FGLNGAEIVFNPSATVGgLSEPMWSIEARNAAIANTFFVGSINRVGTEVFPNPFTSGDGKPQHADFGHFYGSSYFSAPDA 360
gi 30698009   261 FGLNGAEIVFNPSATVGeLSEPMWPIEARNAAIANSYFVGSINRVGTEVFPNPFTSGDGKPQHNDFGHFYGSSHFSAPDA 340
gi 168030450  259 FGMNGAEIVFNPSATVGdLSEPMWPIEARNAAIANSYFVGAINRVGTEVFPNEFTSGDGKPAHKDFGHFYGSSFVAAPDA 338
gi 118361640  858 LGLNGAEIVFNPSATVGgLSEPMWPIEARNAAIANHYFSVGINRVGTEIFPNKFTSGDGGQARNDFGHFYGSSYVAAPDG 937
gi 219481988  244 YGINGAEIVFNPSATVGaLSEPMWPIEARNAAIANSYFSVAINRVGTESFQSEFTSGDGKKAHHDFGHFYGSSYLAAPDG 323
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*..
Feature 1                                                        
2VHI_C        326 SRTPSLSRdKDGLLVVELDLNLCRQVKDFWGFRMTQRVPLYAESFKK 372
gi 169158995  325 SRTPGLSRtRDGLLVAELDLNLNRQVADKWNFKMTGRYEMYAEELKK 371
gi 215504604  324 SRTPGLSRtQDGLLVTELDFNLCRQVRDSWGFRMTQRTEIYADALYW 370
gi 196006812  324 GRTPGLSRvNEGLLVVELDLNLIRQIKDKWGFQMTARLDMYAKELAN 370
gi 74866565   328 CCTPSLSRvSDGLNISEVDLNLCQQVKDKWNFQMTARYELYAKFLTD 374
gi 148907795  361 SCTPSLSRyQDGLIIADMDLNLCRQVNDKWAFRMTARYDMYAELFAS 407
gi 30698009   341 SCTPSLSRyKDGLLISDMDLNLCRQYKDKWGFRMTARYEVYADLLAK 387
gi 168030450  339 SCSPSLSRcRDGLLVAKLDLNLCQQLKDKWGFRMTSRIDMYADFFRE 385
gi 118361640  938 SRTPGLSRtRDGLLVVEVDLNLCQQIKDKWGFTMTGRHEYYAEKLTD 984
gi 219481988  324 SRTPGLSRvDDGLLVTEVDLNLCRQVKDKWCFRMTARLDMYAESLAY 370

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