2EWR


Conserved Protein Domain Family
NT_Pol-beta-like_1

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cd07749: NT_Pol-beta-like_1 
Click on image for an interactive view with Cn3D
Nucleotidyltransferase (NT) domain of an uncharacterized subgroup of the Pol beta-like NT superfamily.
The Pol beta-like NT superfamily includes DNA polymerase beta and other family X DNA Polymerases, as well as Class I and Class II CCA-adding enzymes, RelA- and SpoT-like ppGpp synthetases and hydrolases, 2'5'-oligoadenylate (2-5A)synthetases, Escherichia coli adenylyltransferase (GlnE), Escherichia coli uridylyl transferase (GlnD), poly(A) polymerases, terminal uridylyl transferases, Staphylococcus aureus kanamycin nucleotidyltransferase, and similar proteins. Proteins belonging to this subgroup are uncharacterized. In the majority of the Pol beta-like superfamily NTs, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations essential for catalysis. These divalent metal ions are involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this subgroup.
Statistics
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PSSM-Id: 143394
View PSSM: cd07749
Aligned: 4 rows
Threshold Bit Score: 207.414
Threshold Setting Gi: 91214520
Created: 13-Jul-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
metal binding
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:metal binding triad [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               # #                                      
2EWR_A        17 EYLRVLRKIYDRLKnekVNWVVTGSLSFALQGVp-VEVHDIDIQTDeEGAYEIERIFSefVSKKVRFSSTe--kICSHFG 93
gi 14520626    5 AHLKVLRKLYERLKdsnVNWVVTGSLGFALQGVp-VEPHDIDIQTDkEGAYEIERLFSefVVEPVRFKESe--iIRSHFG 81
gi 108764714   5 RHLRALRAVYEALSgreVRWALTGSLSFALQGAp-VEPDDIDIQTDrEGAYEIQRALHehVTRRVEFSSSaaggIRSHFG 83
gi 91214520    5 IYEEAFRYFLNKIIninIDWALTGSYRLYLENIhnFNPSDIDIITDnKGIELISSIFHknINTEFKYSESn--gIYSYFG 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 #                                                                      
2EWR_A        94 ELIIDGIKVEIXGDIRKRLedGTWEDPVDLNk-YKRFVEthgXKIPVLSl--EYEYQAYLKLGR----VEKAETLRKWLN 166
gi 14520626   82 VLTIDGIKVEIMGDIQKKVe-GKWEPPVDINr-YKRFVQiegMKIPVLDl--EYEYQAYLKLGR----IKKAEMLKKFLE 153
gi 108764714  84 ELRIGGVRVEVMGDVQKRLpdGSWEAPVALEr-HTRRVEvegMSVPVLAl--EYEAAAYRRLGRldtaRMLEEMLEKENR 160
gi 91214520   83 QLQINNTTFDIMSDVKNRIegEWVETPNLSNieYIKFDN---YEIPVLSllsELELCNHLGLNQkknqIEEIIKIKKYAQ 159

                 ..
Feature 1          
2EWR_A       167 ER 168
gi 14520626  154 QR 155
gi 108764714 161 ER 162
gi 91214520  160 QS 161

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