3G3R,3G3O,3G3Q,3G3U,3G3T


Conserved Protein Domain Family
PolyPPase_VTC_like

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cd07750: PolyPPase_VTC_like 
Click on image for an interactive view with Cn3D
Polyphosphate(polyP) polymerase domain of yeast vacuolar transport chaperone (VTC) proteins VTC-2, -3 and- 4, and similar proteins.
Saccharomyces cerevisiae VTC-1, -2, -3, and -4 comprise the membrane-integral VTC complex. VTC-2, -3, and -4 contain polyP polymerase domains. For S. cerevisiae VTC4 it has been shown that this domain generates polyP from ATP by a phosphotransfer reaction releasing ADP. This activity is metal ion-dependent. The ATP gamma phosphate may be cleaved and then transferred to an acceptor phosphate to form polyP. PolyP is ubiquitous. In prokaryotes, it is a store of phosphate and energy. In eukaryotes, polyPs have roles in bone calcification, and osmoregulation, and in phosphate transport in the symbiosis of mycorrhizal fungi and plants. This subgroup belongs to the CYTH/triphosphate tunnel metalloenzyme (TTM)-like superfamily, whose enzymes have a unique active site located within an eight-stranded beta barrel.
Statistics
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PSSM-Id: 143622
View PSSM: cd07750
Aligned: 73 rows
Threshold Bit Score: 115.875
Threshold Setting Gi: 237671569
Created: 16-Jun-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on Saccharomyces cerevisiae VTC4 polyP polymerase
  • Comment:This active site includes the substrate binding and acceptor pockets, and putative metal binding residues.
  • Structure:3G3R_A; Saccharomyces cerevisiae VTC4 polyP polymerase bound with a substrate analog (adenosine-5prime-[(beta,gamma)-imido] triphosphate) and Mn2+, contacts determined at 4.5A.
    View structure with Cn3D
  • Structure:3G3T_A; Saccharomyces cerevisiae VTC4 polyP polymerase bound with orthophosphate, contacts determined at 4A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #                                        #                       # #        
3G3R_A        11 RQTTKYWVHPDNITELKLIILKHLPVLVFntnkeferedSAITSIYFDNEnlDLYYGRLRKd-EGAEAHRLRWYggm--- 86
3G3Q_B        11 RQTTKYWVHPDNITELKLIILKHLPVLVFntnkeferedSAITSIYFDNEnlDLYYGRLRKd-EGAEAHRLRWYggm--- 86
3G3U_A        11 RQTTKYWVHPDNITELKLIILKHLPVLVFntnkeferedSAITSIYFDNEnlDLYYGRLRKd-EGAEAHRLRWYggm--- 86
3G3T_A        11 RQTTKYWVHPDNITELKLIILKHLPVLVFntnkeferedSAITSIYFDNEnlDLYYGRLRKd-EGAEAHRLRWYggm--- 86
gi 229824247  12 RIESKYLVDAKTMTALEKEFSQHLVPDDYp--------vSTISNLYFDNAdyDLVEDSLARl-HGREKVRMRTYdakp-- 80
gi 227513388  46 RREYKYLLNAEEYITIRKLLMGHMSEDQYg--------lTTILSLYFDTPqyYFINQSMKKv-PYKSKFRVRSYgtp--- 113
gi 228476932  11 RIETKYVVSKENLEDLLTDLKEYLVEDDYp--------tSTISNIYFDTDnfDVIQDALDGn-HRQEKIRMRTYlanp-- 79
gi 81450724   12 RKEIKYLIEADTFEQLQKEFKDYLTPDRFt--------hSSITNIYFDNPdfQLIKDSINHr-HASEKIRMRTYdpkp-- 80
gi 229231524  21 RIEKKYLVKRAIWPALSQALHEHLQEGPFg--------rGSVSSLYYDTPrnDMITASMGKp-FYKEKLRIRAYgep--- 88
gi 229880044   8 RKEVKYLLTLEQYETIRQGLVGHMQPDMYg--------rSLIQSVYLDTPd-RSIAVRYDGkqVYKEKLRVRRYvpansn 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                      #            #                                                    
3G3R_A        87 -----stDTIFVERKTHRedwtgekSVKARFALkerhvndFLKGkytvdqvfakmrkegkkpmn-----------eienl 150
3G3Q_B        87 -----stDTIFVERKTHRedwtgekSVKARFALkerhvndFLKGkytvdqvfakmrkegkkpmn-----------eienl 150
3G3U_A        87 -----stDTIFVERKTHRedwtgekSVKARFALkerhvndFLKGkytvdqvfakmrkegkkpmn-----------eienl 150
3G3T_A        87 -----stDTIFVERKTHRedwtgekSVKARFALkerhvndFLKGkytvdqvfakmrkegkkpmn-----------eienl 150
gi 229824247  81 ----kadSFAFLEIKKKIle----vGHKYRFVAdmadiirYVAHptnarpdanpel------------------------ 128
gi 227513388 114 ----lmdEPVFLELKKKIqg----vVYKRRLQIpydalenTLQTglscldhqqlvsne---------------------- 163
gi 228476932  80 ----kidSKVFLEVKAKDde---giGHKFRLVStplvitnLMTDgknhgqitdrqll----------------------- 129
gi 81450724   81 ----tqkSQAFLEIKKKEyqgeqeiGYKYRLTStplslcnYVQQgiidnnikqddra----------------------- 133
gi 229231524  89 ----aadEAVFVELKTKVkg----sSYKRRVATscgaaqaWLAGmpyaeacarfplrstihrtassssmpshhpsesvsl 160
gi 229880044  79 vvpaadcDAVFVELKKKFkg----iTYKRRVHVnegvagaLMLAdksyldaldafmparsvlgep--------artaksv 146
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                               # #                            #                         
3G3R_A       151 ealaseiqyvmlkkKLRPVVRSFYNRTAFQlpg-------------daRVRISLDTELTMVREDnfdgvdrthknwrrtd 217
3G3Q_B       151 ealaseiqyvmlkkKLRPVVRSFYNRTAFQlpg-------------daRVRISLDTELTMVREDnfdgvdrthknwrrtd 217
3G3U_A       151 ealaseiqyvmlkkKLRPVVRSFYNRTAFQlpg-------------daRVRISLDTELTMVREDnfdgvdrthknwrrtd 217
3G3T_A       151 ealaseiqyvmlkkKLRPVVRSFYNRTAFQlpg-------------daRVRISLDTELTMVREDnfdgvdrthknwrrtd 217
gi 229824247 129 --qltllelqrryvALKPMMFIGYKRHSYRgie-------------dpKVRVTIDREVVYRNYDldltagry-------- 185
gi 227513388 164 nqikqeikwlfdryDIKPKVLISDDRVALStde-------------dpDFRVTFDFNIRFRDEDlslhhggy-------- 222
gi 228476932 130 --qdihhlrqrydeGLKPRMYIYYDRFSMKeketie-------gyaynKVRVTLDQNLTYRDEDvslfkgnh-------- 192
gi 81450724  134 --nhelailrerykKLVPKMFIHYERFSLRgie-------------npKLRVTFDRNVIYRHENvnltsgfn-------- 190
gi 229231524 161 qivreidaarkrhaPLSPLIMTIVERVALTsas-------------rsDVRVTFDFDARWRMKDlsfdtgls-------- 219
gi 229880044 147 qiaselvatrdryeGLCPSMLINAVRVAWEpvgasdanihseetagigDVRITFDESIEYADLQhisreaaaqdfgs--- 223
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*...
Feature 1                               #                             #### 
3G3R_A       218 igvdwpfkqlddkdicrfPYAVLEVKLQtqlgqeppEWVRELVGSHlvepvpkFSKFI 275
3G3Q_B       218 igvdwpfkqlddkdicrfPYAVLEVKLQtqlgqeppEWVRELVGSHlvepvpkFSKFI 275
3G3U_A       218 igvdwpfkqlddkdicrfPYAVLEVKLQtqlgqeppEWVRELVGSHlvepvpkFSKFI 275
3G3T_A       218 igvdwpfkqlddkdicrfPYAVLEVKLQtqlgqeppEWVRELVGSHlvepvpkFSKFI 275
gi 229824247 186 -----------geplvddDQVILEIKVNgd----qpDWMVEILNRYqve-eisFSKYG 227
gi 227513388 223 -----------gtkitpdFETIMEVKATga----ypVWFTDIIANLhlv-kgrFSKYG 264
gi 228476932 193 -----------gfplldeDTIIMEIKAPgk----kpDWLQTILDKHgle-eqkFSKYS 234
gi 81450724  191 -----------gypllknNQIVMEIKVKng----lpAWMEKIFHKHqli-pqsFSKYT 232
gi 229231524 220 -----------gtplfadGCIIMEVKCQga----ypLWLAQLLSRSclr-pqpCSKVC 261
gi 229880044 224 ------cfeapshrlledGLVLMEVKVLga----ypMWLSELLSSAkav-kssYTKYG 270

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