3G3R,3G3Q,3G3U,3G3T


Conserved Protein Domain Family
PolyPPase_VTC4_like

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cd07751: PolyPPase_VTC4_like 
Click on image for an interactive view with Cn3D
Polyphosphate(polyP) polymerase domain of yeast vacuolar transport chaperone (VTC) protein VTC4, and similar proteins.
Saccharomyces cerevisiae VTC-1, -2, -3, and -4 comprise the membrane-integral VTC complex. VTC-2,-3, and -4 contain polyP polymerase domains. S. cerevisiae VTC4 belongs to this subgroup. For VTC4 it has been shown that this domain generates polyP from ATP by a phosphotransfer reaction releasing ADP. This activity is metal ion-dependent. The ATP gamma phosphate may be cleaved and then transferred to an acceptor phosphate to form polyP. PolyP is ubiquitous. In prokaryotes, it is a store of phosphate and energy. In eukaryotes, polyPs have roles in bone calcification, and osmoregulation, and in phosphate transport in the symbiosis of mycorrhizal fungi and plants. This subgroup belongs to the CYTH/triphosphate tunnel metalloenzyme (TTM)-like superfamily, whose enzymes have a unique active site located within an eight-stranded beta barrel.
Statistics
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PSSM-Id: 143623
View PSSM: cd07751
Aligned: 19 rows
Threshold Bit Score: 324.314
Threshold Setting Gi: 226460254
Created: 19-May-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on Saccharomyces cerevisiae VTC4 polyP polymerase
  • Comment:The active site includes the substrate binding and acceptor pockets, and the putative metal binding residues.
  • Structure:3G3R_A; Saccharomyces cerevisiae VTC4 polyP polymerase bound with a substrate analog (adenosine-5prime-[(beta,gamma)-imido] triphosphate) and Mn2+, contacts determined at 4.5A.
    View structure with Cn3D
  • Structure:3G3T_A; Saccharomyces cerevisiae VTC4 polyP polymerase bound with orthophosphate, contacts determined at 4A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              #   #                                                                     
3G3R_A         5 KQQNFVRQTTKYWVHPDNITELKLIILKHLPVLVFNTNKEfe---------------------------------rEDSA 51
gi 226460254 231 PIRPFTRKTVKYWVDVRDVLRVKLAVARHLPLLVYDGKKGkkkgkkkgkkngttrgde-ggdgdgdgdgdeifpprDGGK 309
gi 221057892 218 PPETFERTSTKFLMKYEDIVYTKVKIVKHLPYLIFGLSNQdleehfrnfgqkknkklaaqmdgelddtheknkplnESQQ 297
gi 74777302  271 PPETFLRNTTKYWVHPNNVVSAKVGIIRYVPYLIFGISNSeletlldpymcv------------eghnvvdpktieESQA 338
gi 74621188  161 SNCMFIRMTDKYWVHKENVVPVKFYITQHLPIYVFSKGNEekspys------------------------swdknsHDTC 216
gi 169805984 154 TNLVFVRKTNKYWVHIDNLMALKLSIVKNLPLYVFTNEKGqtpysa-------------------------wnhktHDTC 208
gi 226463148 288 GAQDFVRTTRKYWVSPEDVSGVKQNIAQHLPVFLVERERVkglveaaagesgdg--------vapgfagltlpshaDSQM 359
gi 116059043 216 PPSTFERRTVKYWVHPRDVVPLQLEILKRLPILDFRDQRVgkddrek-----------------------dqrdvsDRSM 272
gi 226517202 278 PPTTFERRTRKYWIRPRDVLRVKLATCKHLPVLVFGGRDGraasapsrgak---------------nlalgdvgpvDGCL 342
gi 145349235 114 PPSTFERRTVKYWVDPRDAIPVQLAILKHLPILEFSGGEDaeprae-------------------------ddedqDRSL 168
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #                      # #                                            #     
3G3R_A        52 ITSIYFDNENLDLYYGRLRKdEGAEAHRLRWYGGm------------------------------STDTIFVERKTHRED 101
gi 226460254 310 ITSAYFDSETLDVYKIRLAReQGATLVRARWYGDdadvsgvygdaa-------aardddddddddDDDDVFLERKSHHES 382
gi 221057892 298 ITSVYLDNEEATCYSNRILRyENAQLIRFRWYNDnd---------------------------gnPDKTIFIERKIHHEE 350
gi 74777302  339 ITSVYLDNMNAECYYNRISRfEDAQLFRLRWYGSns---------------------------gsKEKEIFMERKTHHES 391
gi 74621188  217 ISSVYFDNVAFDLYGERLKKlHHSEAIRIRWYTSe------------------------------VPDVVFVERKKHEDG 266
gi 169805984 209 VTSVYFDNDDFYIYDGRLKKnEGAEAIRIRWYGTis----------------------------kETDTVFIERKKHCEG 260
gi 226463148 360 TNSVYLDNVHLELYHARLKKaPRAIAIRLRWYGPa------------------------------PTGSVYVERKTHRES 409
gi 116059043 273 ITSVYLDNSNCEVYRSRLVRdQGATLVRCRWYGGg------------------------------RWDDVFIERKTHHES 322
gi 226517202 343 VSSVYFDDDTLDVYRTRMERlEGATLVRVRWYGGgsstkdpspfaataegaegaegaegadddehGPSRVYVECKTHHES 422
gi 145349235 169 ITSVYLDNPQFDVYRTRLAReQGATLARCRWYGGs-----------------------------aRSDDVFIERKTHHES 219
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #                                                                        
3G3R_A       102 WTGEKSVKARFALKERHVNDFLKGkytVDQVFAKMrkegkk------------pmneiENLEALASEIQYVMLK-KKLRP 168
gi 226460254 383 WSVDDSVKERFKLARRELAGFISG---VIDPSARFas--------------------dASARALATEISRDEIEaRRLTP 439
gi 221057892 351 WTGEISTKERFELQQKYVFKFMTGqmdVPKFFQRRrgtmgkegtqvkgtcvgnlekklKKNEKLAMEIQAMITK-NQLEP 429
gi 74777302  392 WTGEKSAKERFSLPQKYVFGYLKGkfnVDSLIKEGifhtrkn----------qekenvEKSINLAKEIQNYIIN-NKLQP 460
gi 74621188  267 WTGESSKKLRFMINEKQVLKYINGedvWEDVRRLNg---------------------dEDVRMLYEEVQSSIVN-RGLRP 324
gi 169805984 261 WTGEESKKLRFKIEEKYVNDFIQGkdvWKHVSRLNg----------------------KEIKYLYDEIQGAIVK-HTLKP 317
gi 226463148 410 WTGEESVKERFALPAAYIVPYLQCkhtWEKEESRLraqhakndr-----erptsgaelKKIKTLFTEVQRAIES-KQLQP 483
gi 116059043 323 WSGEKSVKERVKISRATAVSIFDG---HTPLRDLP-----------------------ASDSDLINEVRSLEIDrRNIKP 376
gi 226517202 423 WSNESSVKERFRLDARDLKSYLAGg--VAAKSRTSdrtlh--------------qtldAKSATLAKEVAQDQLFaRGLAP 486
gi 145349235 220 WSAQKSVKERCSMRREDAVVLLQGt--PPKALSMS-----------------------KKNLTLAREVLAQEIKrRGVKP 274
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             # #               #                                                   # #  
3G3R_A       169 VVRSFYNRTAFQLPGDARVRISLDTELTMVREDNFDGVdrthknwrrt---digvdwpfkqLDDKDICRFPYAVLEVKLQ 245
gi 226460254 440 SLRVDYRRAAFQTASSNAIRVSLDTELAFTDARRRRPTrtttln------------pnisnPKAAAAVEFPHAVLEVKLH 507
gi 221057892 430 IIRTSYLRSAFQLSDDNSIRISIDTNVSMLNEYVRKREhwcrl--------------aeeaLRKNEIRRLNYAIIEVKLK 495
gi 74777302  461 FVRTSYLRAAFQNQDNNQVRFSIDTNLCMVNEYISDGHqnepwc-----------rlaeevLSKNEVTRFPFAILEVKLQ 529
gi 74621188  325 VVRTFYRRSAFQIPNDSSVRVSLDTDLCMIKECSDAEIvnptypitrwrrsdvncdfpfrnVPKSEIVRFPHAILEIKTQ 404
gi 169805984 318 KIRTFYKRISFQIPNDSTIRLSLDTNLCMIKENGENWRrtdimc-----------ewpfrqLEDTEIVRFPHAILEVKTQ 386
gi 226463148 484 TLRTVYMRTAFQVPYDASVRCSLDTSLAMLVENPAGGPsctalerw--------yrdpsvpLHRTEVTRFPHAVLEIKLA 555
gi 116059043 377 VLMTKYRRTAFQRSDGNQIRISIDTELQWRDVRDLGTRais------------------ksSLHDFSPPFAYAVLEVKLS 438
gi 226517202 487 SVGTRYRRTAFQRADTNDVRVSLDVDLVFTDARDCSYPr---------------------dGSRRAPVRFPCAILEVKLA 545
gi 145349235 275 TLLTQYRRTAFQRSDGNQLRVSFDTDLRWRDVRGEGSGvls-----------------naaFKGGEPAAFRFAILEVKLA 337
                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
Feature 1                                      ####                             
3G3R_A       246 tq----lgQEPPEWVRELVGSHL-VEPVPKFSKFIHGVATLLNdkv----------dsIPFWL 293
gi 226460254 508 e-------GEKCEWIDEALRAAPaSTEVYKFSKFLHGTVATRGgvndetpgggaflwrIPHWF 563
gi 221057892 496 v-------EKIPQWVQDILNSNH-FINAYKYSKYQTAMALLHPeki----------kyIPVWI 540
gi 74777302  530 --------SEQPIWVSEMLKRCN-ATLVYKFSKFQHGMAILHEdkv----------siFPHWI 573
gi 74621188  405 nf-----dETKPKWIEELINGPL-VEHVHKFSKYLHGCAVLYPfi-----------qdIPYWL 450
gi 169805984 387 gv-----dESKPTWLEDILNSTY-VEHVYKFSKFLHGIAILYNvk------------dIPYWL 431
gi 226463148 556 lg----egVAAPDWVNELVASGA-LTEVHKFSKFMHGCAVLFPdta----------qeVPYWV 603
gi 116059043 439 cs----eeEELMEWIDEVTSTFD-VIQVHKFSKFLHGCAMHFPrda---------lhtLPHWY 487
gi 226517202 546 radgddagGGTPDWIEDVLAAGN-ATEVKKFSKFLHATAALRVdtngg------vdaaLPHWY 601
gi 145349235 338 te-----dAKSPEWIENIISSFN-VTHVYKFSKFLHGCAMLFYpnea--------lpkLPHWY 386

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