3BHD,2JMU,3BHD


Conserved Protein Domain Family
ThTPase

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cd07758: ThTPase 
Click on image for an interactive view with Cn3D
Thiamine Triphosphatase
ThTPase is a soluble cytosolic enzyme which converts thiamine triphosphate (ThTP) to thiamine diphosphate. This catalytic activity depends on a divalent metal cofactor, for example Mg++. ThTPase regulates the intracellular concentration of ThTP, maintaining it at a low concentration in vivo. ThTP acts as a messenger in cell signaling in response to cellular stress, and in addition, can phosphorylate proteins in certain tissues. There is another class of membrane-associated enzymes in animal tissues which also convert ThTP to thiamine diphosphate, however they do not belong to this subgroup. This subgroup belongs to the CYTH/triphosphate tunnel metalloenzyme (TTM)-like superfamily, whose enzymes have a unique active site located within an eight-stranded beta barrel.
Statistics
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PSSM-Id: 143625
View PSSM: cd07758
Aligned: 19 rows
Threshold Bit Score: 168.324
Threshold Setting Gi: 167537795
Created: 5-Jun-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 29 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site pocket [active site]
Evidence:
  • Comment:The active site pocket based on NMR titration experiments of mouse ThTPase. It includes the Mg2+ and ThTP binding sites, and key hydrophobic residues.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #####                                   #             # # #        # #         
3BHD_A        25 IEVERKFLpg----pgTEERLQELGg----TLEYRVTFRDTYYDtpelsLXQADHWLRRREDs---GWELKCPGaagv-- 91
2JMU_A         6 IEVERKFApg----pdTEERLQELGa----TLEHRVTFRDTYYDtselsLMLSDHWLRQREGs---GWELKCPGvtgv-- 72
3BHD_B        25 IEVERKFLpg----pgTEERLQELGg----TLEYRVTFRDTYYDtpelsLXQADHWLRRREDs---GWELKCPGaagv-- 91
gi 148232734  11 IEVERKFVpg----pqVEDKLHSLGa----KLQNELTFRDSYYDtpdcrLTLGDVWLRRRED----SWELKYPPqrgtrg 78
gi 126631523   9 VEVERKFVcd----aeIMRKLQDIGa----ECVGEREFGDQYFDspdflLTLKDFWLRCRDG----QWELKSPAvseap- 75
gi 215494033  52 CEVERKFRap----cdIEDRLVRIGa----VLGSRTQFTDRYYDtqdnvLALNDHWLRIRGGas-dHWELKYRKhndtps 122
gi 226463632  63 IEVERKYGa------pADLKAAITTae--gTLTKSIIFGDTYYDtpncdLAAKDVWLRRREK----QWEIKIPVegeggr 130
gi 156368104   9 LEIEKKFQip----vrFEETLLSNGg----KLTERREFQDVYYDlpdnsLTLKGLWLRKRNT----KWELKHLNarcdp- 75
gi 225717448  92 IEVERKFTvp----ecFEALLDKHGfknmsGFEQDEEICDTYYDsqgfdLMIEDYWLRSRNG----DWELKYPVgvhp-- 161
gi 219121725  41 FEVEQKFPlsldslpiLEERLRILGf----EQITTKELTDWYFDnvcsdLVQQDCWLRYREDenqgEWQLKKGVrgqyn- 115
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               # #                                                        # #           
3BHD_A        92 -lgphTEYKELTAEPTIVAQLCKVlradglgagd---------------vaavlgplgLQEVASFVTKRSAWKlvllgad 155
2JMU_A        73 -sgphNEYVEVTSEAAIVAQLFELlgsgeqkpag---------------vaavlgslkLQEVASFITTRSSWKlalsgah 136
3BHD_B        92 -lgphTEYKELTAEPTIVAQLCKVlradglgagd---------------vaavlgplgLQEVASFVTKRSAWKlvllgad 155
gi 148232734  79 lngasTQYLELNCERDIAHRVSEElnipcph---------------------sldlleLQEFASFVTKRRRFElpprn-- 135
gi 126631523  76 aetlcTRYREITSLPVIQSEVSKIiresncsqrkqidqted-tpicstgdtkwlnelgLVCFAEFRTQRCSYVlere--- 151
gi 215494033 123 ndsavTIYNEETGEANILTIVKKLlperspvgeav-------------tlkdfvseglLREFACIQTWRSSYKts----- 184
gi 226463632 131 sggerSVFREVEGARSVADELAAAlglpapsdggdggga-----dgeeiltrvmadaaARPFAEFVTERVSWSld----- 200
gi 156368104  76 tisitDKYHETSDETKIMTELSKHvpmatsl--------------------delvskhCREFASFTTQRTSYQlp----- 130
gi 225717448 162 --tgsTLYHETSDVHEISAKLQILvpkknngskrnsm--------ifnnigdficnqiLHKFAELITRRKHYQkd----- 226
gi 219121725 116 gantsTVYEEIEGAKALNAAVDLLakverkadvplpfefagfapfhipklleeisllqLHPFARILTKRASWVstdqcn- 194
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               ## #  #  # # # ##                                                       #
3BHD_A       156 eeepqLRVDLDTADFGYAVGEVEALVhe---eaEVPTALEKIHRLSSXLGVpa---------------------qETAPA 211
2JMU_A       137 gqepqLTIDLDSADFGYAVGEVEAMVhe---kaEVPAALEKIITVSSMLGVpa---------------------qEEAPA 192
3BHD_B       156 eeepqLRVDLDTADFGYAVGEVEALVhe---eaEVPTALEKIHRLSSXLGVpa---------------------qETAPA 211
gi 148232734 136 gskhkVVVDLDTADFGFSVGEVEVLVet---qeEVQSALKEVEEICKKLGVls---------------------dSPVPG 191
gi 126631523 152 --dgaVRVDLDRADFGYCVGEIEVLVpe---ggDVNAALQSITSIAEQLGLns---------------------eKKVKG 205
gi 215494033 185 ---grSVVDLDQTDWGYRVAEIEVLLqeadcdaQGKDAMEKATEEISRLAAqldgkplfinpkhaarelgraqfeTRIPC 261
gi 226463632 201 ----gASIDFDHASFGHSVVEVEVMCer---aeQVEDAEKKVEEVAAKLGLep---------------------lTETGG 252
gi 156368104 131 ---ndVRVDLDVASFGYSVGEIEIVIdd---etGVSEAEQRLEGLAEKLGInw---------------------qKKFPG 183
gi 225717448 227 ----nVSIVVDETNWGYKVGEIEMVVkd---klEVPEASKKIDAIARQLNFkkl-------------------klPLIAS 280
gi 219121725 195 mmfwaLTVDLDITDFGYAVGEVEAMIkd---rrDVPRARAAINGLVKRLKPvgt-------------------hsTIAIG 252

                 ....*.
Feature 1        ####  
3BHD_A       212 KLIVYL 217
2JMU_A       193 KLMVYL 198
3BHD_B       212 KLIVYL 217
gi 148232734 192 KMSTFL 197
gi 126631523 206 KMDVFL 211
gi 215494033 262 PRTAFR 267
gi 226463632 253 KLETFI 258
gi 156368104 184 KVEEFL 189
gi 225717448 281 KISTRE 286
gi 219121725 253 KLEYYL 258

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