2FBL


Conserved Protein Domain Family
CYTH-like_CthTTM-like

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cd07761: CYTH-like_CthTTM-like 
Click on image for an interactive view with Cn3D
Clostridium thermocellum (Cth)TTM and similar proteins, a subgroup of the CYTH-like superfamily
CthTTM is a metal dependent tripolyphosphatase, nucleoside triphosphatase, and nucleoside tetraphosphatase. It hydrolyzes the beta-gamma phosphoanhydride linkage of triphosphate-containing substrates including tripolyphosphate, nucleoside triphosphates and nucleoside tetraphosphates. These substrates are hydrolyzed, releasing Pi. Mg++ or Mn++ are required for the enzyme's activity. CthTTM appears to have no adenylate cyclase activity. This subgroup consists chiefly of bacterial sequences. Members of the CYTH-like (also known as triphosphate tunnel metalloenzyme (TTM)-like) superfamily have a unique active site located within an eight-stranded beta barrel.
Statistics
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PSSM-Id: 143626
View PSSM: cd07761
Aligned: 25 rows
Threshold Bit Score: 163.556
Threshold Setting Gi: 227991152
Created: 28-May-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:
  • Comment:Based on mutational analysis of Clostridium thermocellum TTM. These residues are important for tripolyphosphatase and ATPase activity.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         # #                                            # #                #         # #
2FBL_A         4 TEIERKFLV------ATFPDg------eLHAVPLRQGYLTtptdsIELRLRQQg------tEYFMTLKSEGg-lSRQEYE 64
gi 227991152   3 REIERKFLVr----sPEWKTg-------AAGVLYRQGYLCrq-eaRTVRVRIAg------gQAYLTIKGQAqgvTRLEYE 64
gi 237684713   3 EEIERKFLL------NELPAdia---twPQPAAILQGYLQkd-qhKDLRIRKKg------nKYTLTAKHGTg-lVREENE 65
gi 218251529   1 MEIERQFLV------DIIPAl------pDTFDSILQGYVSl---fPEIRIRSVrple-gpeKFYLTVKRGEg-lVRDEWE 63
gi 160945352   1 MEIERKWMV------TGWPEgl----plEEEFTMRQGYISv---qPTVRIREEaltg-gstDYILCFKSAGg-lAREEIE 65
gi 223038655  31 IEIERKFILrdaaviSELKArd----igTEQKEVTQIYVKit-plEEIRFREAs------gVFTITQKSGIg-lAREENE 98
gi 160933222   1 MEIERKFLL------SGFPDlp-----eLERAEVFQGYLGi---sPEVRIRSKrvg--eaeTYRLCFKGEGs-lVREEIE 63
gi 229828375   1 MEIERKWIV------TDWPRgl----slLYEQTMRQGYLSv---rPTVRIREEkmsd-getHDILCFKSGSg-iVRREIE 65
gi 182417210   1 MEIERKWLF------KSIAApkg--tkiLGEYFYKQAYISi---nPEVRIRSRrkaeddnfTYALCIKSKGt-lERIEVQ 68
gi 154175291   1 MEIERKFLLk----nSQILEflsaesvsFKRLEILQFYTKit-dnEEVRYRSEd------aKFIKTIKIGKd-lVREENE 68
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                # #              #            # #                       
2FBL_A        65 IQIDVTQFEMLWp---ATEGRRVEKTRYSGKlp-dGQLFELDVFAGHLSPLMLVEVEFLsedaAQAFIPP-----PWFGE 135
gi 227991152  65 YPIPLAEAQELLe--hLCLKPLIEKTRYCIEf--rGRVWEVDEFAGENQGLVVAEVELEs--pDQPLELP-----DWVGE 133
gi 237684713  66 QEIDKTLFDILWp---FTAEARIEKLRYTRVi--dEGEMVIDKYLGDLSGLLLMEVEFNdeqlARTYSPP-----LNFAK 135
gi 218251529  64 TEISSREFSHLVec-lEKGTWFIEKRRYRLPla-gDHVAEYHRHSGHLKGFDYVEVEFRseeeARAFEPP-----YWFGR 136
gi 160945352  66 RSIDKDLFVQLEe--kIIGKPLIKKVRRSYRlpdgAVLEVNHVDEGLPTAFWYAEVEYPtveaALSWQPAacglvAYLND 143
gi 223038655  99 SETDAKSFKKALk---NVVAAPIKKTRFLFRl--dGAACNVDIFHGALEGLVTLEAEFAsereAAEFNPPef-iaAHIAS 172
gi 160933222  64 LDLDKATFESLKk---LLPKPMIRKEYRIYRlengLRLECSRVDPGTDTEFYYAEVEFQdqqqAEIFSPP-----DFLGK 135
gi 229828375  66 IEISPDQFQELEe---MIALPLIPKIRRTYAlsdgLKLEVNHVDCGSDTEFWYAEIEYPteeaARSWQPSpl-laSYLGK 141
gi 182417210  69 KELTEKEFNELMvvgnIKEEDFIEKHFYKYSvn-nHELTLGVTDIGRSTEFFYGEIEFNssedAISFTPP-----KWFGQ 142
gi 154175291  69 CECDKKEFKNAFk---SHIGAPIYKDRYLFKl--nNNPCNIDVFKGAQEGLCTLEVEFKdeaeAVYFNLPkf-lrNFIEA 142
                        170
                 ....*....|....*
Feature 1                       
2FBL_A       136 EVTEDKRYKNKALAL 150
gi 227991152 134 EVTHDPRYLNASLVE 148
gi 237684713 136 EVTDDPRYRNAALAA 150
gi 218251529 137 EVTEDPRFSYGKLAR 151
gi 160945352 144 EVTNQPGQSMGAYWV 158
gi 223038655 173 EVTEDERYKNKNLAL 187
gi 160933222 136 EVTYDGSYRMKAYYQ 150
gi 229828375 142 DVSEEPGQSMGAYWL 156
gi 182417210 143 EVTDDPQYKMSNFWQ 157
gi 154175291 143 DVTCDQRYKNKYIAL 157

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