Conserved Protein Domain Family
CYTH-like_CthTTM-like_1

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cd07891: CYTH-like_CthTTM-like_1 
CYTH-like Clostridium thermocellum TTM-like subgroup 1
This subgroup contains the triphosphate tunnel metalloenzyme (TTM) from Clostridium thermocellum (CthTTM) and similar proteins. These are found primarily in bacteria. CthTTM is a metal dependent tripolyphosphatase, nucleoside triphosphatase, and nucleoside tetraphosphatase. It hydrolyzes the beta-gamma phosphoanhydride linkage of triphosphate-containing substrates including tripolyphosphate, nucleoside triphosphates and nucleoside tetraphosphates. These substrates are hydrolyzed, releasing Pi. Mg++ or Mn++ are required for the enzyme's activity. CthTTM appears to have no adenylate cyclase activity. This subgroup consists chiefly of bacterial sequences. These enzymes are members of the CYTH-like (also known as triphosphate tunnel metalloenzyme (TTM)-like) superfamily, which have a unique active site located within an eight-stranded beta barrel.
Statistics
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PSSM-Id: 143629
View PSSM: cd07891
Aligned: 77 rows
Threshold Bit Score: 160.746
Threshold Setting Gi: 118470067
Created: 10-Aug-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:
  • Comment:Based on similarity to Clostridium thermocellum TTM, where these residues have been shown by mutation analysis to be important for tripolyphosphatase and ATPase activity.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         # #                                   # #                                  #   
gi 227991152   3 REIERKFLV-RSPEWKTg-AAGVLYRQGYLCr-qEARTVRVRIAg------------------------gQAYLTIKGQA 55
gi 118470067   9 FEFERKFFLrELPAVAAsdPSPTLIVQAYLFa-aDGYAVRIRVQqpaptdlgagl----dtflqdlhdesIGTMTAKGPA 83
gi 227428547  11 FEFERRFLVrELPATLR--DTPALIVQSYFLs-dGGFALRVRVQasgvearlsassdarevlarhtgqfdFAAVTVKGPS 87
gi 213691233   8 FEYERRFFCrELPAEYDdgDAPTLIIQSYYVh-aDNYALRVRLVshkvhvdmtpdvnpvavldeyrdrfsEAYITVKGPS 86
gi 87301839    3 LEIERRFLV-GGTGWREhiLWRQHLCQGYLQasaDGVTVRVRLEdgrte----------------aaqgeRAWLTLKATV 65
gi 33866799    3 VEIERRFLL-NGEDWRSlaGEPQPLRQGYLAasaEGITVRIRLRga-----------------------eAAWLTLKAAA 58
gi 33239631    3 LEIERRFII-KGNEWKAfiKQSQELEQGYFStnfEEWIVRMRMIdr-----------------------kKSQITLKALA 58
gi 72383358    3 IEIERRFLV-ANEDWKDqvIRSESVSQAYLNssvDEWATRVRIIdn-----------------------nKGYITLKSSL 58
gi 159902713   3 IETERRFLV-KNKEWQVliKKCIKIRQGYLStdfQEWITRIRIInn-----------------------qRSYITIKNSK 58
gi 148243381   3 LEIERRFFV-VSEAWQElsVKHEQLDQGYLSegqGAVVVRVRRSq------------------------qHAWLTLKAPT 57
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                # #                      # #            #            # #                
gi 227991152  56 qg--vTRLEYEYPIPLAEAQELLehLCLKPLIEKTRYCIEfrGRVWEVDEFAGENQGLVVAEVELESPDQPLELPDWVGE 133
gi 118470067  84 vg--gTRYEAERQLDPLVAAEIV--RRAEHVVAKIRYSMWlgEDGWIVDRFLGGNAPLVLAEVERGGPVVDLAIPAFCVS 159
gi 227428547  88 vg--gTRYEAERQIEPSVGVELV--RRGGAQILKTRYAAWlgADGWVIDEFGGTNHPLLVAECERSGPVTDLQIPDFCVT 163
gi 213691233  87 vg--gTRYEVEREIDTRIAAELI--KRGGSVIIKNRYSVWiaEDGWSVDVFGGPNAPLIVAEAERSGPVTNLTIPKFCIT 162
gi 87301839   66 ta--vTRAEFEYAIPVADARQLL--GLAAASLSKERYGLDlpGGDWVLDVFEGENSPLVVAEVELEREDQVLALPAWCHR 141
gi 33866799   59 daiglVRHEFEYAIPVADAESLW--TLAPHRLEKTRYQLSlaGGDWVVDHFAGRNGPLLLAEVELPAAQTPLEIPSWCGQ 136
gi 33239631   59 gk--mSTHEFEYSIPLEDAVFMW--NLISKKLKKKRYFLNfgPGSWVVDCFEGSNFPLVLAEVELNSEKVLFEQPSWCSQ 134
gi 72383358   59 ng--lANYEFEYSIPRKDAIELI--QLSKYKITKIRYQLKinKKNWVVDVFEESNSYLKIAEIELNSESEEIQVPSWCGK 134
gi 159902713  59 gg--mQSHEFEYKIPIHDAESIW--HLLNYKLIKQRYLLDlnPGSWVVDCFKNENSPLVIAEVELEAPELDVMIPDWCGK 134
gi 148243381  58 nn-peIRQEFEYTIPAADAKALL--ALTPRRVEKTRHHLNcpGGDWVVDVFAGANAPLVIAEVERSDPEAQLTIPSWCGE 134
                        170
                 ....*....|....*..
Feature 1                         
gi 227991152 134 EVTHDPRYLNASLVEHP 150
gi 118470067 160 EVSEDDRFRNEYLAFQP 176
gi 227428547 164 ELTDDRRFSNESLAVDP 180
gi 213691233 163 EITDQARFNNDGLANRP 179
gi 87301839  142 EITGLGALSNAALARRP 158
gi 33866799  137 EITGDGRWSNAALARRP 153
gi 33239631  135 EVTGIKKLSNASLAQYP 151
gi 72383358  135 EITGIKSLSNASLAKAP 151
gi 159902713 135 ELTGQKQWSNAALAKFP 151
gi 148243381 135 EITGRGELSNAALAMRP 151

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