3G3O


Conserved Protein Domain Family
PolyPPase_VTC2-3_like

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cd07892: PolyPPase_VTC2-3_like 
Click on image for an interactive view with Cn3D
Polyphosphate(polyP) polymerase domain of yeast vacuolar transport chaperone (VTC) proteins VTC-2, and -3 , and similar proteins.
Saccharomyces cerevisiae VTC-1, -2, -3, and -4 comprise the membrane-integral VTC complex. VTC-2, -3, and -4 contain polyP polymerase domains. S. cerevisiae VTC-2,and -3 belong to this subgroup. For VTC4 it has been shown that this domain generates polyP from ATP by a phosphotransfer reaction releasing ADP. This activity is metal ion-dependent. The ATP gamma phosphate may be cleaved and then transferred to an acceptor phosphate to form polyP. PolyP is ubiquitous. In prokaryotes, it is a store of phosphate and energy. In eukaryotes, polyPs have roles in bone calcification, and osmoregulation, and in phosphate transport in the symbiosis of mycorrhizal fungi and plants. This subgroup belongs to the CYTH/triphosphate tunnel metalloenzyme (TTM)-like superfamily, whose enzymes have a unique active site located within an eight-stranded beta barrel.
Statistics
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PSSM-Id: 143630
View PSSM: cd07892
Aligned: 11 rows
Threshold Bit Score: 471.079
Threshold Setting Gi: 223645268
Created: 7-Aug-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on similarity to Saccharomyces cerevisiae VTC4
  • Comment:This active site includes the substrate binding and acceptor pockets, and putative metal binding residues.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  #                                                                     
3G3O_A        36 NDIDXNFRSFKFWVHNDNLXEVKTRILRHLPVLVYANVPSenddlvnrfesdisnndei----------------vgsss 99
gi 74623235  206 THNGEKYTAYKFWVHPDNLLEVKTYILRRLPALVYSHQSSkew------------------------------------- 248
gi 3183344   195 NSSMYRRRTFRFWVHPDNVMEVKTYIMRHLPVLYYSGKQGfdkdqn---------------------------------- 240
gi 74604513  187 NDSDLNYTSYKFWVHSDNLMEVKTRILRHLPILVYNNQNDsq-------------------------------------- 228
gi 190344512 182 EDTTQDVKSFKFWIHRDNLMEVKATILRHLPVLVYNNDNYeddddd---------------------------------- 227
gi 159130946 210 RSGQDAYSSYKFWVHEDNILEVKTYILRRLPVLIYNPKSSrdfe------------------------------------ 253
gi 223645268 207 GATNGEFQSFKFWIHPDNLMEVKTTILRHLPVLIYNSTNNddddddddde------------------------------ 256
gi 189196074 218 NAVGEDFTSYKFWVHMDNLFEVKTVILRRLPVLVYNPQTSkvae------------------------------------ 261
gi 238031391 215 DSGSDNFKVLKFWVHPENLMEIKTTILRHLPALIFTDESGddd------------------------------------- 257
gi 14318518  197 NDIDMNFRSFKFWVHNDNLMEVKTRILRHLPVLVYANVPSenddlvnrfesdisnndei----------------vgsss 260
gi 6325238   199 NLEDASFKSYKFWVHDDNIMEVKARILRHLPALVYASVPNenddfvdnlesdvrvqpearlnigsksnslssdgnsnqdv 278
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                             #                       # #                #              #
3G3O_A       100 stssvehglgarsFDPLINTLYFDNEHFELYNDKLLKl-NSAPTLRLRWTGQLs-DKPDIFLEKKTLIedeatgkseFDL 177
gi 74623235  249 ----------dgdEDPTITSLYFDNANFQLYYQKVERe-AEASSLRLRWYGQLs-QNPEIVFEQKIIHdn-----gtSEE 311
gi 3183344   241 --------gvsgiLDPISTCLYLDNSNFDLYSQNLERs-EQAYSLRLHWYGKLt-PKTDIIVERMVRQgst----lsHSE 306
gi 74604513  229 -----------dsVDPTLTSLYFDNSDFELYEGKLQKn-DTSPSLRLKWAGKTsdEDSQIYIEKRTVDhs-----iaGGD 291
gi 190344512 228 --------ddassNDPVINTLYFDNPNFDLYNDKLIKk-ANAATLRIKWIGKLl-DNPKISVEKKSFDpts---dtfDVN 294
gi 159130946 254 ----------slpEDPTITSLYFDNPQFDLYNQKVAKa-PEAGSLRLRWTGDLk-DTPAIFLEKKIVTsd-----dySKE 316
gi 223645268 257 ---deddsnkhitGGQTINCLYLDNDHFDLYNHKLTKl-NNSSTVRIRWVGKLa-DKPKISMERKSFDvns----nfFVD 327
gi 189196074 262 ----------gtqRDPTITSIYFDNPNFSLYTDKVNGk-PDAASLRLRWYGHLn-EKPEIVFEKKVIKeg-----daAEE 324
gi 238031391 258 -----------dlSDPVISTLYFDNRGFELYNNKLLKnlKETPTLRIRWTSKLt-PGKDVTVEKRTFLnet----gvDDD 321
gi 14318518  261 stssvehglgarsFDPLINTLYFDNEHFELYNDKLLKl-NSAPTLRLRWTGQLs-DKPDIFLEKKTLIedeatgkseFDL 338
gi 6325238   279 eigksksvifpqsYDPTITTLYFDNDFFDLYNNRLLKi-SGAPTLRLRWIGKLl-DKPDIFLEKRTFTentetgnssFEE 356
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
3G3O_A       178 TKLQLKQKFINGFIFEgd----------------------kKFKEQTLKklkesgtagrdlERLEEDFSEIQNFIIKNEL 235
gi 74623235  312 RKFPIKEKYIKPFLDGe-----------------------yKMEKSIQKmerqgq-spadvEEFRSTAEAIQDYIRSNKL 367
gi 3183344   307 DRFTIREKKVRELLSGr------------------------YDFRKVEDdhst-----tasDQKKKLIEDVEQLIVDNHL 357
gi 74604513  292 ERLPIKEKYIAGFINGt-----------------------yHMEKTVQKmksshv-pdyqvEGYEKSVENIQDFIRENSL 347
gi 190344512 295 EKVVLKEKYLDTFVKGn-----------------------fSPEKLIKKmekr----gensKDFADTVQNLESFITEHHL 347
gi 159130946 317 VKVQLKKKHVQEFLEGk-----------------------yTFEKQLHRmeglnngesaeaETLKKDVEELQSFIKENNL 373
gi 223645268 328 DKIELRQKYINQYVIQk------------------------EIPKKLVK------------LNDANSVKKLLDFIKEYNL 371
gi 189196074 325 VRFPIKAKYVQSFLEDh-----------------------yHMEKSIEKmeyrpnkdqkklDNFKKAVTDIQGFIKEQKL 381
gi 238031391 322 IKLKLKEKYINDFIKLqsdeddeesldhstdyasskefnvnKFTSKLTKrnlp----qltiQDERQKFISLQKFIREQRL 397
gi 14318518  339 TKLQLKQKFINGFIFEgd----------------------kKFKEQTLKklkesgtagrdlERLEEDFSEIQNFIIKNEL 396
gi 6325238   357 IRLQMKAKFINNFIFKndpsyk-------------nylinqLRERGTQKee---------lEKLSRDFDNIQNFIVEEKL 414
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               # #                #                                                     
3G3O_A       236 QPVFRTVYTRTAFQI-PGDDKIRVTIDSNIVFIKEDSFDRERPIRDPNTWHRTDIDanv-anPLKFLRGGEYAKFPYSVX 313
gi 74623235  368 EPVVRANYVRTAFQN-PGDDRIRLSIDTDIAFIREDTLDRDRPCRDPNNWHRTDIDdsnmtyPFKNINQSEVSLFPYAVL 446
gi 3183344   358 QPVLRSVYTRTAFQI-PGDDEVRINLDSDWVMIREDSLDIERPCRDPEDWHRHDIDda--dfPYKHLRKGEYSRFPYSVL 434
gi 74604513  348 QPVCRAQYTRTAFQI-PGDNRVRVIMDNDILFIKEDAFDKDRPIRDPNNWHRTDIDav--sdPKKVLRKGEYSKFPYAVL 424
gi 190344512 348 QPAVRSVYRRTAFQI-PGDDKIRVVIDSDLMFIREDSFDEQRPIRDPKKWHRTDIDski-snPYSLLRSGEYSKFPYSVM 425
gi 159130946 374 QPMLRANYTRTAFQI-PGDDRIRISLDTNLALIREDALDEERPCRDPTDWHRRDIDdsgmqyPFDGIRTGEVNRFPHALL 452
gi 223645268 372 QPILRTTYNRTAFQI-PGDDRIRIIIDSNLTFIREDSFDPQLPIRDPQQWHRLDLDns--ksPQQFLRKGEFVKFPFSTM 448
gi 189196074 382 QPMVRANYTRTAFQI-PGDDRVRISLDTNLALIREDALDLDRPCRDPDDWHRRDIDnaqmefPFKAIKKGEIHKFPYALL 460
gi 238031391 398 EPVLRTVFTRTVFQVpGDEDKIRVVIDSDMLYIREDSFDKDRPIRDPKSWHRRDLDqpnlvnPFDVLREGEYAKFPYSIM 477
gi 14318518  397 QPVFRTVYTRTAFQI-PGDDKIRVTIDSNIVFIKEDSFDRERPIRDPNTWHRTDIDanv-anPLKFLRGGEYAKFPYSVM 474
gi 6325238   415 QPVLRATYNRTAFQI-PGDQSIRVTIDSNIMYIREDSLDKNRPIRNPENWHRDDIDsni-pnPLRFLRAGEYSKFPYSVM 492
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
Feature 1        # #                                             ####                         
3G3O_A       314 EIKVKssldssxsas--sxisnvklpKKHGQWLNDLTNSHL-VKEIPKFSIFVQGVASLYgdde-kldiLPFWLPDL 386
gi 74623235  447 EIKLKed-----------------qnRKRPVWVTDLMASHL-VHPCPRFSKFMQGVASLFedy---vnrLPFWLPDL 502
gi 3183344   435 EIRECvr-----------------ydEDEPLWISELRNSHL-ISEIDGFSKYEHGVAILFeky---vslLPMWVFSM 490
gi 74604513  425 EFRVQnrpgvdtadtgnsiitsssvyRKHGKWIDELTSSHL-VKEVPKFSKFIQGIASLYgedd-klnfLPLWLSVL 499
gi 190344512 426 EIKIKts-----------------knNKRLLWVDELVHSHL-VKEVPNFSKFVHGVSVLFledd-klqnIPMWYHDL 483
gi 159130946 453 EIKLRg-------------------sAHHIEWVNELMVSHL-VKEAPRFSKFVHGVAQLFedh---vnsFPFWLSEL 506
gi 223645268 449 EIKIKkss---------------aknFKKLQWVNELINSSYlVKEIPNFSKFIHGVASLFledd-kldnIPMWFNEL 509
gi 189196074 461 EIKVKg--------------------MKKYEWIEDLMHSHL-VKESPRFSKFVHGVAKLFedn---vntFPFWHQRG 513
gi 238031391 478 EIRVNeriy-------------snpnSKTYKWVRDLVNSHL-VKEVPNFSKFIQGISSLFledd-nldiLPFWLPDL 539
gi 14318518  475 EIKVKssldssmsas--smisnvklpKKHGQWLNDLTNSHL-VKEIPKFSIFVQGVASLYgdde-kldiLPFWLPDL 547
gi 6325238   493 EIKVInqd---------------nsqMPNYEWIKDLTNSHL-VNEVPKFSLYLQGVASLFgeddkyvniLPFWLPDL 553

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