1EWJ,1MH6,1ECS


Conserved Protein Domain Family
BLMT_like

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cd08350: BLMT_like 
Click on image for an interactive view with Cn3D
BLMT, a bleomycin resistance protein encoded on the transposon Tn5, and similar proteins
BLMT is a bleomycin (Bm) resistance protein, encoded by the ble gene on the transposon Tn5. This protein confers a survival advantage to Escherichia coli host cells. Bm is a glycopeptide antibiotic produced naturally by actinomycetes. It is a potent anti-cancer drug, which acts as a strong DNA-cutting agent, thereby causing cell death. BLMT has strong binding affinity to Bm and it protects against this lethal compound through drug sequestering. BLMT has two identically-folded subdomains, with the same alpha/beta fold; these two halves have no sequence similarity. BLMT is a dimer with two Bm-binding pockets formed at the dimer interface.
Statistics
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PSSM-Id: 319938
View PSSM: cd08350
Aligned: 24 rows
Threshold Bit Score: 170.151
Threshold Setting Gi: 946935870
Created: 27-Jan-2010
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
ligand bindingdimer interface
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site [chemical binding site]
Evidence:
  • Structure:1EWJ_A/B; bleomycin binding sites of BLMT from Transposon Tn5.
    View structure with Cn3D
  • Comment:The BLMT dimer has two bleomycin binding sites.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                         # #      # #              #   #                
1EWJ_A         2 TDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLMLEFFAHPgLDPLASWFSCCLRLDDLAEFYRQCKSVGI 81
gi 756550641  14 SLRATPNLPSRDFAATADFYARLGFETRFRSDGWMILGRGDVEVEFFHHPgLDPRSSWFSACLRTLDLDALYAEFRTAGL 93
gi 739383789   3 TGKITANLPSRDFDATERFYATLGFQTEFRNAGWMILSRDGMTVEFFPHLdLDPGTSCFSACLRLPEIDSLHAEWRNATG 82
gi 666618208   2 IGYSTPNLPSKNLNKTYEFYKRLGFNRTYNSESWMILEMHDIVLEFFPHQtLTLEDNWFSCCIRLSDVPTFFNHALSIGV 81
gi 640617264   3 PDRITANLPSRDFDETLRFYEQLGFTLHFRNDGWMIMGRGTMQLEFYPHPkLVAAENWSSACIRVDDLDGLHTQWSVLDL 82
gi 640628741   3 PDRVTANLPSRDFDRTCAFYGLLGFDPLYRDDGWLILRRGTMEVEFFAHPdLVLAESWFSACIRVDDLDGLRTSWHALDL 82
gi 497271803   2 QDYISANLPAIDFDHTRKFYAFLGFQCDYQSDQWMILTKDSLILEFFHHPqLDPKASWFSACLRTHVMQDLFHFWQALDW 81
gi 262315535   6 EGQITANLPAIDLACTASFYQSLGFDLVYQSAEWMIMRLGEMTLEVFHHPhLDPNSSWHSACIRVAEIQPYYEYWSKVNW 85
gi 926240125   1 MDYISANLPAIQLDETRDFYSALGFQCTYFSAEWMILEQGSLKLEFFHHPeLDPQASWHSACIRVRDLAALYHTWHEVNW 80
gi 948113356   2 TDHATPNLPSRNFEHTQKFYGSVGFTTTYVDDSWLIMVRGGITLEFFLHTdLDPQTSWFSCCLRVDNVDEMFAAFKAAGI 81
                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
Feature 1                              #   #   #   # # #    
1EWJ_A        82 Qets-sgyPRIHAPElq----eWGGTMAALVDPDGTLLRLIQN 119
gi 756550641  94 Tdds-raiPRLTPPVeq----pRVPRYFALVDRDGSLWRCLQT 131
gi 739383789  83 LpdtrgaiPRLTDPFtl----dHAPRMFALVDPDGSLWRILES 121
gi 666618208  82 Plsd-sgyPRITAPKgd---snPSVLISYLIDIDGNLIRLIQS 120
gi 640617264  83 Pd-----dPNGRPLNmdmiggaGQPRFFPVIDCNGSLLRCIDN 120
gi 640628741  83 Pddp-mdrPRDLTIQgg----dGKPRFFVLIDCDGSLIRCIDN 120
gi 497271803  82 Tqy---pdARMSEIQy-----lEEIDMFCVVDPNGSLIRCIQL 116
gi 262315535  86 Slw---esARMTDIQd-----fDEIRQFCIIDPNGSLLRCIEV 120
gi 926240125  81 Slf---pqARITEIQs-----lAEIDLFCVIDINGSLLRCIQH 115
gi 948113356  82 Gedp-hsrPCVHLPEtq----sWGMKMGVMIDIDGSLIRVIEN 119

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