Conserved Protein Domain Family
HycB_like

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cd10554: HycB_like 
HycB, HydN and similar proteins
This family includes HycB, the FeS subunit of a membrane-associated formate hydrogenlyase system (FHL-1) in Escherichia coli that breaks down formate, produced during anaerobic fermentation, to H2 and CO2. FHL-1 consists of formate dehydrogenase H (FDH-H) and the hydrogenase 3 complex (Hyd-3). HycB is thought to code for the [4Fe-4S] ferredoxin subunit of hydrogenase 3, which functions as an intermediate electron carrier protein between hydrogenase 3 and formate dehydrogenase. HydN codes for the [4Fe-4S] ferredoxin subunit of FDH-H; a hydN in-frame deletion mutation causes only weak reduction in hydrogenase activity, but loss of more than 60% of FDH-H activity. This pathway is only active at low pH and high formate concentrations, and is thought to provide a detoxification/de-acidification system countering the buildup of formate during fermentation.
Statistics
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PSSM-Id: 319876
View PSSM: cd10554
Aligned: 69 rows
Threshold Bit Score: 148.947
Threshold Setting Gi: 78217928
Created: 29-Jan-2014
Updated: 18-Aug-2016
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 77417745    4 FIIADASKCIGCRTCEVACVVSHqenqdca----sltpetFLPRIHVIKgvNISTATVCRQCEDAPCANVCPNGAISRD- 78
gi 494792407   9 VVFAEADKCKACKKCELACIASHnnmtike---avkkrnlFQPRVHVIKtdSVKMPVQCRQCADAPCARVCPTDALVQE- 84
gi 494737640   5 FIFADPKKCIGCLNCELACAASHegidieeayeralagekMIHRNEVVKlnDLTAPIQCMQCENAPCVEACPIDIIKYE- 83
gi 517009692   8 FIHADLSRCLSCKSCELACGLAHagyeieg---aaagqmkLKPRVSVVQqgTTVALTQCRQCEDAPCVKVCPNGSLYQE- 83
gi 518844571   7 ALVVDAEKCKGCKKCEIACVEAHtnltrke---iiknkatSLSRIKVCNieKAKVPVQCHQCHNAPCARVCPTAALVRE- 82
gi 495957535   4 HIQVVPDKCRACRRCEVACIAAHhgmsfke---amkhrdvLVARVQVVKaeGFKTTVRCHQCDPAPCCNVCPTGALQQEe 80
gi 328452233   8 FLLVDPQRCMGCHTCELACAAAHtragsiig--avlarerLHRRNRVVQvdGVKLPVQCRQCQDAPCVRVCPTGATYRT- 84
gi 584601915   5 FILADSTKCVGCLSCELACAASYqgiafee---ayttkvpLISRNRVVKveTKTAPLQCMHCEDAPCAIICPHGVIERM- 80
gi 268617856   5 FIITQTDACIGCLSCELACAAAHanisfee---ayttslpLLSRNRVIQsyGKMAPLQCMHCETPSCLAICPHGVISLE- 80
gi 488449732   8 LVISNPSHCLGCKGCELACATGKlnkyian---gqsdkrnIIANITVTEndNFKFPAYCRHCKDAYCLKMCPTKAIKEV- 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 77417745   79 KGFVHVMQERCIGCKTCVVACPYGAMEVVvrpvirnsg---aglnvradKAEANKCDLCnh-----rEDGPACMAACPTH 150
gi 494792407  85 DGVVVMRQQYCAACQLCVMACPYGAIELSfiglpeegaa-daaqpaqprREVAVRCDVCadwrakegKDQGACVEACPVK 163
gi 494737640  84 GDYVKIYEEDCIGCRSCAIVCPFGAVVMAespy------------dnvsGLIAMKCDLCgg-----vENTQACVEICPTH 146
gi 517009692  84 EGLVKLNRETCIGCKLCARACPFGSITMTtevvera-------dgkknnRTKALKCDLCfsrnkeikAEGCACIQACPTK 156
gi 518844571  83 PGMVRVRQQLCVGCKMCVMVCPFGAINVAhnepiig-------datptsRKVAIKCDQCsewrakngEELTACAKACKFG 155
gi 495957535  81 DGRITMRVQFCVACKMCIAACPYGTISLDtigmpdlsgsdaetlaqrarREVAVRCDMCrawreengKKITACMEACPVR 160
gi 328452233  85 ETYTAVDQARCIGCRLCMMVCPFGAIHVAtttv------------agqeRRAAFKCDLCvn-----rLGGPACVAACPTQ 147
gi 584601915  81 DGFIKLHEEACVGCGCCSLVCPYGAISMIqk----------------edRRVAVKCNLCfs-----hPEGPSCVRVCTTD 139
gi 268617856  81 EGFIKLDETACVGCGCCALACPYGAISMVke----------------deRVYALKCNLCfe-----rDNNPACVEHCPTH 139
gi 488449732  84 EGIIKLTEDKCIGCGICKQACPYGAINMTsyte------------nsklKKVAVKCDMCier--qlnNEDPLCYSACPTK 149

                 ....*
gi 77417745  151 ALICV 155
gi 494792407 164 ALHMV 168
gi 494737640 147 AIELI 151
gi 517009692 157 VLSLS 161
gi 518844571 156 AIKFV 160
gi 495957535 161 ALSMV 165
gi 328452233 148 ALSLR 152
gi 584601915 140 AIRLV 144
gi 268617856 140 ALSLM 144
gi 488449732 150 ALKLI 154
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