2IVF


Conserved Protein Domain Family
EBDH_beta

?
cd10555: EBDH_beta 
Click on image for an interactive view with Cn3D
beta subunit of ethylbenzene-dehydrogenase (EBDH)
This subfamily includes ethylbenzene dehydrogenase (EBDH, EC 1.17.99.2), a member of the DMSO reductase family. EBDH oxidizes the hydrocarbon ethylbenzene to (S)-1-phenylethanol. It is a heterotrimer, with the alpha subunit containing the catalytic center with a molybdenum held by two molybdopterin-guanine dinucleotides, the beta subunit containing four iron-sulfur clusters (the electron transfer subunit) and the gamma subunit containing a methionine and a lysine as axial heme ligands. During catalysis, electrons produced by substrate oxidation are transferred to a heme in the gamma subunit and then presumably to a separate cytochrome involved in nitrate respiration.
Statistics
?
PSSM-Id: 319877
View PSSM: cd10555
Aligned: 18 rows
Threshold Bit Score: 554.22
Threshold Setting Gi: 747130620
Created: 30-Jan-2014
Updated: 18-Aug-2016
Structure
?
Program:
Drawing:
Aligned Rows:
 
Fe-S clustertrimer
Conserved site includes 49 residues -Click on image for an interactive view with Cn3D
Feature 1:Fe-S cluster binding site [ion binding site]
Evidence:
  • Structure:2IVF: Aromatoleum Aromaticum ethylbenzene dehydrogenase binds to 4[Fe-S] clusters; contacts at 4A distance.
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                     #######   #   #           ## #                                     
2IVF_B        15 AKRQLVTVIDLNKCLGCQTCTVACKNIWTKRPGTEHM-RWNNVTTYPGKGYPRDYErKGGGFLRg-------EPQPGVLP 86
gi 381216162   9 TQRQVSMVLDLNKCLGCHTCSIACKKMWNTDPGTDYT-YWNNVETMPGKGYPANYP-QIGGRTEt------gKVKRGQIP 80
gi 123627056   8 PKRQLTYVTDLNKCIGCQTCTVACKKLWTTGPGQDFM-YWRNVETTPGLGYPRNWQ-TKGGGYKn------gELQKGKIP 79
gi 556698664  27 VDHQVAMVMDLNKCIGCQTCTVACKSLWTEGGGRDYM-YWNNVETKPGAGYPRDWE-ESGGGWQssd---hsERRPGEIP 101
gi 528864759   2 SKRQVAMVMDLNKCIGCQTCTVACKTLWTRREGMEHM-LWNTVNTQPGAGTPHNFE-KMGGGFKd------gVAQPGIRP 73
gi 821326891   9 PNRQLTYVVDLNKCIGCQTCTVACKKLWTTSPGQEFM-YWRNVETTPGLGYPRNWE-KKGGGYKng-----vLQKDGHKP 81
gi 816615946   2 SKRQLAMVIDINKCLGCHTCTIADKNMWLRGEGQEYM-CWNNVETRPGKGFPANWE-RLGGGWKd------gEIHLGKLP 73
gi 728903473   5 PKRQLTFIVDLNKCIGCQTCTVACKKLWSTAPGQEFM-YWRNVETTPGLGYPKNWQ-SKGGGYKng-----mLQTDGHYP 77
gi 816977647   3 IMKQIRMVADLNKCLGCQTCTMSCKTMWTDRNQGQTHmYWINVETRPGKGYPKNWE-TQGGWFTad-----rNNIPLPNI 76
gi 158509039  29 SNRQIGMVMDLNKCIGCQLCSMACKTLWTSKEGREYM-WWNKVNTMPGKGSPKDWE-KMGGGYKvkfggkvmEPVQGKHP 106
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                            # #####  ###
2IVF_B        87 tlidsgDDFQFNHKe----vFYEGKGQTVhfhptsk--stgkdpaWGYNWDEDQGGGKWPNp-FFFYLARMCNHCTNPAC 159
gi 381216162  81 nm--dtGYGRSWSFnhkdvlASTGQPVKLwikpk-------eevkWGPNFDEDEGTGHYPQdnHYFYLPRLCNHCTHPAC 151
gi 123627056  80 pm---iDYGIPFEFdya-grLFEGKKERVrpsp---------tprSAPNWDEDQGAGEYPNn-SFFYLPRMCNHCTKPAC 145
gi 556698664 102 dk---eEYGEAWEFnhe-evMYEGSDQPLrpes---------dpeWGPNWDEDQGAGEHPNs-YYFYLPRICNHCTHPSC 167
gi 528864759  74 tv---eDFGKAWEFnhk-dvFFGGKSDISlaadg-------grhgKGPNWDEDQGGGEFPNs-HYFYMPRICNHCTHPAC 141
gi 821326891  82 tl---dEYGVPFEYdya-arLFEGKKGRVkpsp---------eprWAPNWDEEQGTGEYPNn-MFQYLPRMCNHCENPAC 147
gi 816615946  74 al---eDYGIPWSWdw---eQLPHIAPQVap-------------tWGPNWEEDVGAGDYPNv-WYFYLARPCNHCTKPAC 133
gi 728903473  78 tl---dEYGVPFKYdfa-arLFEGKKGSVkpsp---------eprWAPNWDEDQGVGEYPNn-IFAYLPRMCNHCTNPAC 143
gi 816977647  77 n----eDYGAPWEYnh--heVLKTEGGDSqasnlipspvpsgsnaYASNWDEDVGKGTFPNs-YYFYLPRICNHCTDPPC 149
gi 158509039 107 tr---kEFGDMWDYnw--ndVVNNKAGAVhlqaknq--tdgsvpdWSMNWDEDQGAGQYPNg-FYFYLPRICNHCAYPAC 178
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           # #  #        #      #######   # # ##        # #####               ###   ##  
2IVF_B       160 LAACPTGAIYKREDNGIVLVDQERCKGHRHCVEACPYKAIYFNPVSQTSEKCILCYPRIEKGIA------NACNRQCPGR 233
gi 381216162 152 LDACPRGAIEKREHDGIVLVNQDRCHGYRFCVEACPYKKVYFDHVRQVSAKCIFCAPRVEEGVA------PACARQCPGR 225
gi 123627056 146 LEACPNEAIYKREQDGIVVIHQDKCKGAQACVQSCPYAKPYFNPVANKANKCIGCFPRIEQGVA------PGCVAQCVGR 219
gi 556698664 168 VEACPRKAIYKREEDGIVLIDQERCRGYRYCVEGCPYKKVYYNAAQKTSEKCIFCYPRIEGEGPdgetfaPACAEDCPPQ 247
gi 528864759 142 LEACPRKAITKREEDGIVLIDEDRCKGYRFCQEACPYKKIYYNEMEGVSQKCIFCFPRIDEGVT------TACSRQCPGR 215
gi 821326891 148 LESCPNDAIYKRAEDGLNIVNLDKCKGRQECIKACPYGKVYFNTTDQKANKCIGCFPRIEKGVA------PACVAQCVGR 221
gi 816615946 134 VAACPRKAVYKRDEDGIVLIDQERCRGYRYCVRACPYKKPYFNAIRYKSEKCIFCFPRVEQGVP------PACVGQCPGR 207
gi 728903473 144 VEACPTDSIYVRAEDGLKVINLEKCVGVKECVKACPYGKIYFNETDHQSNKCIGCFPRIEKGVA------TACVAQCVGR 217
gi 816977647 150 LEACPRQAIYKRKEDGIVLIDPKRCRGYRYCIKGCPYKKIYYNPVEKISQKCIFCYPRIEQRKG------NFCAAQCVGR 223
gi 158509039 179 VDACPRNAIYKRDEDGVVLIDQDRCKGYRFCLEACPYKVIYFNFLTDTSMKCIFCYPRLDEKVA------NACARQCTAR 252
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ##                                                                              
2IVF_B       234 VRAFGYLDDTtSHVHKLVKKWKVALPLHAEYGTGPNIYYVPPMGARGFGEDGEitdk-triPLDVLEGLFGPe------- 305
gi 381216162 226 LRFVGYRDDQdGPIWKLVEKYQVALPLHGEWGLGPNVFYVPPMSPSKMDDRGRptde-priPLAYLESLFGPr------- 297
gi 123627056 220 AMHVGFIDDTnSSVHKLIRLYKVALPLHPEFGTEPNVFYVPPVLGPRMELPNGelstdpkiPLAQLEGLFGKq------- 292
gi 556698664 248 LRLVGFLDDEaGPVHKLVEEYEVALPLHPEYRTQPNVYYIPPFAPPQHSDDGEsidv-eriPRPYLEELFGEr------- 319
gi 528864759 216 VRFVGYLDDPeGPIWKLVTKWKVALPLHAEYGTEPNVFYVPPLSPPKLDEDGNatde-priPTEYLVSLFGDg------- 287
gi 821326891 222 AMHVGFIDDTeSSVYKLAKLWKVAMPMHPEFGTKPNVLYIPPFVGPLKEDENGnmlatqkmPLEVLQGLFGPd------- 294
gi 816615946 208 IRFFGYLDDReGPVYKLVKEFQVALPLHPELVTEPNVYYIPPLSPPRVDAQGRpgge-griPVEYLESLFGPq------- 279
gi 728903473 218 AMHVGFIDDVnSSVYKLAKLWKVAMPLHPEFGTEPNVLYIPPFVGPAMQDEKGnmmetqkmPIGVLQGLFGSk------- 290
gi 816977647 224 IYWVGYAEDAnSNVYKLVNKWKVALRLHPEYKTEPNVFYIPPLSPPTYSFAGKltts-priPIEMLAKMFGDtydqsydq 302
gi 158509039 253 VRWIGYTEEPeSIIYKLTKVWKVALPLHAEYGTNPNVFYVPPLAPHRLAPDGKidpskpriPREYLRYLFGPe------- 325
                        330       340       350
                 ....*....|....*....|....*....|...
Feature 1                                         
2IVF_B       306 ----VKRVLAVLHTERENMRAGRGSELMDLLIS 334
gi 381216162 298 ----VKDVLNLLHAERDKKKRGEKSELMDLLIA 326
gi 123627056 293 ----VRDVLAILQTEREKKMKGLASDLMDVLIG 321
gi 556698664 320 ----VHDALDRIARERDEVDRGGESELLDLLTD 348
gi 528864759 288 ----VHHALETVRREQDKSRRGEPSELMDLLIA 316
gi 821326891 295 ----VVGVLDILRAEREKKMAGQGSALMDTMIG 323
gi 816615946 280 ----VRQVLSRLEAELAAARLGKPSELVATLIS 308
gi 728903473 291 ----VGGVLAVLKAERAKKIAGKKSELMDIMIG 319
gi 816977647 303 rvarIRQISDIIQSEREKVSSGGSSELIDILIS 335
gi 158509039 326 ----VDASLDIMQKELDKVRAGGRSELMEILKA 354

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap