1Y4Z


Conserved Protein Domain Family
NarH_beta-like

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cd10557: NarH_beta-like 
Click on image for an interactive view with Cn3D
beta subunit of nitrate reductase A (NarH) and similar proteins
This subfamily includes nitrate reductase A, a member of the DMSO reductase family. The respiratory nitrate reductase complex (NarGHI) from E. coli is a heterotrimer, with the catalytic subunit (NarG) with a molybdo-bis (molybdopterin guanine dinucleotide) cofactor and an [Fe-S] cluster, the electron transfer subunit (NarH) with four [Fe-S] clusters, and the integral membrane subunit (NarI) with two b-type hemes. Nitrate reductase A often forms a respiratory chain with the formate dehydrogenase via the lipid soluble quinol pool. Electron transfer from formate to nitrate is coupled to proton translocation across the cytoplasmic membrane generating proton motive force by a redox loop mechanism. Demethylmenaquinol (DMKH2) has been shown to be a good substrate for NarGHI in nitrate respiration in E. coli.
Statistics
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PSSM-Id: 319879
View PSSM: cd10557
Aligned: 17 rows
Threshold Bit Score: 726.078
Threshold Setting Gi: 116062723
Created: 30-Jan-2014
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Fe-S clustertrimer
Conserved site includes 50 residues -Click on image for an interactive view with Cn3D
Feature 1:Fe-S cluster binding site [ion binding site]
Evidence:
  • Structure:1Y4Z: Escherichia coli nitrate reductase A biinds to 4[Fe-S] clusters; contacts at 4A distance.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                     #######   #   #          ## #                                      
1Y4Z_B         3 IRSQVGMVLNLDKCIGCHTCSVTAKNVWTSREGVEYAWFNNVETKPGQGFPTDWENQEKYKGGWIRKINGKlqprmgnra 82
gi 521976469   5 VMAQVGMVMNLDKCIGCHTCSVTCKQAWTNRAGTEYVWFNNVETRPGQGYPRRYEDQERWRGGWVRTKSGGlklraggrv 84
gi 2105094     3 VRAHMSMLFHLDKCIGCHTCSIACKNLWTDRKGAEYMWWNNVETRPGAGYPTGWEDQERFRGGWEYKDGHLdlrlhsr-t 81
gi 18161786    3 VRAQITMAMNLDKCIGCHTCSVTCKNVWTNRAGAEYMWWNNVETRPGPGYPRQWEDQNKYNGGWALDGGKLklk------ 76
gi 495756146   3 IRAQVSMTFHLDKCIGCHTCSIACKNIWTDRKGTEYMWWNNVETKPGTGYPTLWEDQSKYRGGWERTGDKIrlklqgr-r 81
gi 494026574   3 IKVQYAMVMNLDKCIGCHTCSIPCKNVWTSREGAEYMWFNNVETKPGIGYPGKWENQQIYRGGWTVKGNRLklaaggr-i 81
gi 625007302   3 VMAQLAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQGYPRTYEDQERWRGGWIRDKKGRlrlrdggrl 82
gi 938511316   3 IRAQVSMVFHLDKCIGCHTCSVACKNIWTDRKGTEYMWWNNVETKPGTGYPTLWQDQDKYKGGWEKKEDKLslklqgr-i 81
gi 780798054   3 IRSQIAMVFHLDKCIGCHTCSVACKNIWTDRKGAEYMWWNNVETKPGTGYPTKWEDQDIYQGGWKKDGDSVslkgagk-l 81
gi 288892955   3 VRAQLVMVFNLDKCLGCHTCSIACKNIWTDRRGAEYMWWNNVETKPGAGYPKFWEDQKRYKGGWVLRGDKLklrafsk-l 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1Y4Z_B        83 mllgkIFANPHLPGIDDYYEPFDFDYQNLhtapegsksQPIARPRSLITGERMakiekGPNWEDDLGGefdklAKDKNFD 162
gi 521976469  85 qkllsIFASPVQPELDDYYEPWTYDYANLvsap-lgddFPVARPKSLITGEDTrv-twSANWDDSLGGsqesmAADPIVE 162
gi 2105094    82 qglfrLFFNPALPSLDDYYEPFTFRYADLftap-egedQPTAVPVSMITGEPMtp-eaGPNWDDDLSGsplyaKNDPNLK 159
gi 18161786   77 ---ieLTKNYKPPSLKDYYEPWTYDYEALfsdk-qtdqQPVARPISLITDEPMdv-ayGPNWNDDLAGtd-yiLEDPNLD 150
gi 495756146  82 aglanIFFNPYLPTVDDYYEPWTYQYEDLfnap-egddQPTARPISLITGKPIdi-eaGPNWDDDLGGstvyaANDPNKA 159
gi 494026574  82 hkamnIFHNPDLPVIDDYYEPWDYDYDRLiksp-pknhQPTVRSHSCLTGEPMdp-awGPNWEDDLAGlsetgAGDVNFN 159
gi 625007302  83 hkllrIFANPKLPLIGDYYEPWTYDYENLttap-agdtFPTAAPKSAITGEPMki-swGPNWDDNLAGspeilAEDPILK 160
gi 938511316  82 galanIFFNPSLPTIDDYYEPWTYRYEELfnap-pgddQPTARPVSLITSRPIdi-eaGPNWDDDLGGsqlyaKNDPVLK 159
gi 780798054  82 kglknIFHNPNMPVLDDYYEPWAYRYQDLfdap-egddQPIGRAVSLITGEPIei-qsGPNWDDDLSGtpdyaRQDVNFK 159
gi 288892955  82 stllnIFYQPHMPRLEDYYHPWTYDYAKLyesp-eesdQPVAEPISLISGEKIdi-efGPNWDDDLGGsplyaEEDINLD 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                              #  ####  ###   ##     #  #        # #    #######   # #  # 
1Y4Z_B       163 Niqka----mysQFENTFMMYLPRLCEHCLNPACVATCP-----SGAIYKREEDGIVLIDQDKCRGWRMCITGCPYKKIY 233
gi 521976469 163 KlrdeanekirmSYEQSFMFFLPRICEHCLNPSCMASCP-----SGAIYKRSEDGIVLVDQNRCRGWRQCITGCPYKKIY 237
gi 2105094   160 Alapev-raqleEIEGVVFQYLPRICNHCLNPACVAACP-----SGAIYKRAEDGVVLVNENKCKAWRMCVAACPYKKVY 233
gi 18161786  151 Glqre----iyaQFKDVFMMYLPRICNHCLNPSCLAACP-----RKAIYKREEDGIVLIDQNRCRGYRYCVAACPYKKVY 221
gi 495756146 160 Vltdee-krqlnELERLIFFYLPRICNHCLNPGCVAACP-----AGAIYKRGEDGIVLISQEKCRAWRMCVSGCPYKKTY 233
gi 494026574 160 Rleid----tylKFKNLFMFWLPRLCEHCLNPACVASCP-----SGALYKRDEDGIVLVDQERCRGWRYCVSGCPYKKVY 230
gi 625007302 161 Kvsee----vklSLEETFMFYLPRICEHCLNPSCVASCP-----SGAMYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVY 231
gi 938511316 160 Nsteee-rhllnEVERNVFFYLPRICNHCLNPGCVAACPagavnPGAMYKRGEDGIVLISPEKCRAWRMCVSGCPYKKVY 238
gi 780798054 160 Hlsqae-qeamfQLEKMTFHYLPRICNHCLNPACVASCP-----SGALYKRGEDGVVLINQERCRAWRMCVTACPYKKSY 233
gi 288892955 160 Erri------vtNFGSIFMFYLPRICNHCLNPACVAACP-----QRAIYKRGEDGIVLVDQNTCRGWRFCVTACPYKKVY 228
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               # #####         ###   ### ##                                             
1Y4Z_B       234 FNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADaier-aastENEKDLYQRqlDVFLDPNDPKVIEQA 312
gi 521976469 238 FNHRSGKAEKCTFCYPRLEVGLPTVCAETCVGRLRYVGIVLYDPDavta--aasVPDEELYDAqlGLLLDPHDPEVIADA 315
gi 2105094   234 YNWATGKSEKCILCFPRLETGQAPACAHSCVGRIRYMGVLLYDADripe--aamVPDEELVESqlSLILDPFDPEVIAAA 311
gi 18161786  222 YNWKTGKSEKCILCYPSVEAGQPTVCSLTCVGKIRYMGVLLYDADkvldvaatpDPAQLVRRFidEVLLDPFDPAVIEAA 301
gi 495756146 234 FNWATGKSEKCILCYPRLESGHAPACFHSCVGRIRYLGLVLYDADkiqe--tasVPDRDLVEAqrAIIQDPFDPKIIAAA 311
gi 494026574 231 FNWKQGRAEKCIFCYPRIEAGLPTLCAHSCVGRIRYVGVLLYDADrile-aaavPRDKDVYPAhlDILMDPNDPETIQAA 309
gi 625007302 232 FNHKTGKAEKCTLCYPRMEVGLPTICSETCVGRLRYLGVVLYDVDrvlq-aasvEKETDLYPAqcEILLDPNDPEVIAGA 310
gi 938511316 239 YNWATGKSEKCILCYPRLESGHAPACFHVCVGRVRFLGILLYDADriqe--tasVNDTDLVEAqrAIIQDPFDFQVIAAA 316
gi 780798054 234 YNWNTGKSEKCVLCYPRLESGQPPACMHSCVGRIRYLGVMLYDADriqa--vasCDESEIVDRhmDIYLNPNDPTVIKAA 311
gi 288892955 229 YNWNTGKSEKCIFCYPRTETAQANACAHSCVGRIRYVGVVLYDADried--alkVKDEELVEAqrKIILDPRDEMVRKAA 306
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                                                                
1Y4Z_B       313 IKDGIPlsVIEAAQQSPVYKMAMEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADA 368
gi 521976469 316 RAGGMAedWIAAAQRSPVYALIKHFKVALPLHPEYRTMPMVWYIPPLSPVVDLLKE 371
gi 2105094   312 KAEGIDegWIQAAQKSPIYKFVKTWGLALPLHPEYRTLAMMFYVPPLSPVVATVEK 367
gi 18161786  302 RKNGIPdyWITAAQRSPVYKIVKKWKVGFPLRPEFRVLPMVFYVPPLSPVVTTFEK 357
gi 495756146 312 LANGVTanQIDSAQRSPVYKFVKKWKIALPLHPEFRTLPMLFYVPPMGPMQAAVSG 367
gi 494026574 310 KAQGIAsnVLSAARRSPVYSLIRQWRLALPLHPEYRTLPMVWYIPPLSPVSRQVAD 365
gi 625007302 311 RAEGIPdeWIEAAQRSPVYALIHTYKVALPLHPEYRTMPMVWYIPPLSPVVDAVSR 366
gi 938511316 317 KANGISdaMIDSAQKSPVYKFVKQWKIALPLSPQFRTLPMLFYVPPLLPVLATMKN 372
gi 780798054 312 RENGIAdsTIKAAQNSPVYKFVKEWKIALPLHPEFRTLPNLFYVPPLLPAMASVST 367
gi 288892955 307 KENGVPdnWLEAALKSPAYALIKEYELALPLHPEFRTMPMTFYIPPLSPVLAVHRA 362

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