1KQG


Conserved Protein Domain Family
FDH-N

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cd10558: FDH-N 
Click on image for an interactive view with Cn3D
The beta FeS subunit of formate dehydrogenase-N (FDH-N)
This subfamily contains beta FeS subunit of formate dehydrogenase-N (FDH-N), a member of the DMSO reductase family. FDH-N is involved in the major anaerobic respiratory pathway in the presence of nitrate, catalyzing the oxidation of formate to carbon dioxide at the expense of nitrate reduction to nitrite. Thus, FDH-N is a major component of nitrate respiration of Escherichia coli. This integral membrane enzyme forms a heterotrimer; the alpha-subunit (FDH-G) is the catalytic site of formate oxidation and membrane-associated, incorporating a selenocysteine (SeCys) residue and a [4Fe/4S] cluster in addition to two bis-MGD cofactors, the beta subunit (FDH-H) contains four [4Fe/4S] clusters which transfer the electrons from the alpha subunit to the gamma-subunit (FDH-I), a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups.
Statistics
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PSSM-Id: 319880
View PSSM: cd10558
Aligned: 12 rows
Threshold Bit Score: 345.912
Threshold Setting Gi: 517983909
Created: 3-Feb-2014
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Fe-S clustertrimer
Conserved site includes 50 residues -Click on image for an interactive view with Cn3D
Feature 1:Fe-S cluster binding site [ion binding site]
Evidence:
  • Structure:1KQG: Escherichia coli formate dehydrogenase N binds to 4[Fe-S] clusters; contacts at 4A distance.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          #      #######   #   #                                # #                  #  #
1KQG_B         31 AKLIDVSTCIGCKACQVACSEWNDIrde--------vghCVGVYDNPADLSaKSWTVMRFSEteqn--gklEWLIRKDGC 100
gi 113529894   29 AKLIDVSICIGCKACQVACNEWNDLrgd--------vqeNVGVYDNPRDLSpNTWTLMRFTEhedq-agklEWLIRKDGC 99
gi 14523057    46 AKLIDVSKCIGCKACQSACVEWNDThpg--------igeNVGYYTNPHDLTeDMFTLMRFTEwvnpetdnlEWLIRKDGC 117
gi 2983533     34 AILVDVSSCIGCKACEAACQQWHDTtppilsp-eevikrKIAGYQTHPDLLpETFMLMKFKEgets--rgfTWFITKYQC 110
gi 517983909    8 AILVDTSLCTGCKACQLACKEWHNLpate-------fekVPGTYQNPADRSvNTLMLMKFTEkydrktetfEWLIQKEQC 80
gi 701779629  791 AKLIDVTTCIGCKACQVACSEWNDIrde--------vghNVGMYDNPADLTaKSWTVMRFSEveqn--dklEWLIRKDGC 860
gi 747132536   28 AKLIDVTKCIGCKACQVACMEWNDTrda--------vgtNHGVLDNPADLTaDSWTVMRYSEvetd--rglEWLIRKDGC 97
gi 861642410   15 TKLIDVSICTGCKACQAACDEWNDLrtk--------vghNYGVYDNPMDLEpETWTVIRFDEyvde-ngrlEWLMRKDGC 85
gi 659915521   30 AKLFDATKCNGCKACQVACSEWNDLree--------vgsFQGTYQNPASLSpECWTLMKFNEiedq--gklRWQFTHSAC 99
gi 116223126   30 AKLIDTTTCIGCKACEVACMEWNDLpfr--------etvFDNTYQTMPETAwNYWNLIQFREhvhe-dgtlQWLMRKDQC 100
gi 931436507   12 AILYDPTRCTACRGCQAACKQWNENneyiptvengvqsaNWGSYENPPDLSpTTWLKMKFREidpg--gkvRWLFIRRSC 89
gi 931515142   18 AILFDPALCTACRACQVACKQWNANgav--------qttSRGSYENPSDLTpETWLKIEFREvern--gnfDWLFTRRAC 87
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         ###  ###   ###  ##       #      #######   ### ##            # #####             
1KQG_B        101 MHCEDPGCLKACPSaGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNke----dNRVYKCTLCVDRVSVGq----E 172
gi 113529894  100 MHCEDPGCLKACPApGAIVQYANGIVDFQSDLCIGCGYCVAGCPFDVPRISkl----dNKAYKCTLCSDRVSVGq----E 171
gi 14523057   118 MHCADPGCLKACPApGAIVQYTNGIVDFVHENCIGCGYCIKGCPFNIPRISkv----dHRAYKCTLCSDRVAVGq----G 189
gi 2983533    111 MHCREPGCLIACPSpGAVIQYQNGVVDFDHSKCIGCKMCLVGCPFDIPRYDk-----nNKPWKCNFCIDRVSAGl----E 181
gi 517983909   81 MHCSDAPCVEVCPS-SALYTHADGFVGFQSDKCIGCAYCGKACPFDVPRYEsdpltgkKKMNKCDFCQDRVTNGe----M 155
gi 701779629  861 MHCADPGCLKACPSeGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDVPRLNpe----dNRVYKCTLCVDRVTVGq----E 932
gi 747132536   98 MHCGNPGCLKACPApGAIVQYSNGIVDFHEENCIGCGYCITGCPFNIPRLSer----dSKVYKCTLCSDRVASGl----E 169
gi 861642410   86 MHCEDPGCLKSCPApGAIVQFENGIVDFESEHCIGCGYCMIGCPFNIPRVShk----dNRSYKCTLCSDRVAVGq----E 157
gi 659915521  100 MHCADPACLKACSTsGAIVQHANGTVDFDSDKCIGCGYCASACPFDIPKISav----dNKAYKCTMCSDRLAVGl----E 171
gi 116223126  101 MHCADPGCLAACPAdNAIVQYQNGIVDFQQDYCIGCQYCVTGCPFNIPKFNpq----tKKMFKCTLCNDRVSQGl----E 172
gi 931436507   90 MHCTDAGCVKVCPS-KALYHHELGFVAYNKDLCTGCGYCIEACPFDVPRSErnifsgiAKMDKCTLCTTPGLDRiaegwE 168
gi 931515142   88 MHCTDAACVRVCPT-GALRHHELGFVTYHKDLCSGCGYCRDFCPFDTPRSErdfltglAKMEKCTLCMTPGLDRvaagyE 166
                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
Feature 1         # #   ###  #                                                       
1KQG_B        173 PACVKTCPTGAIHFGTKKEMLELAEQRVAKLka-rGYEHAGVYNPegvgGTHVMYVLHHADQPELYH 238
gi 113529894  172 PACVKTCPTGAIAFGSKAEMQSLAGERVAELne-rGYARAGLYDPagvgGTHVMYVLQHADDPKRYA 237
gi 14523057   190 PACAKACPTQAIVFGTKEDMKKHAEHRIADLks-rGYTNAGLYDPpgvgGTHVMYVLHHSDKPHIYS 255
gi 2983533    182 PACVKTCPTNCLSFGFKEDMIERGKKIVERLke-rGYPNATLYDPpgvgGTGYIYVLPHGDHVKDYQ 247
gi 517983909  156 PACVQTCPTTALTFEHWEEAVKQAYQRVEKIk--eRYPNANVYGDkhmgGTHYIYVLTEKPEVYGLP 220
gi 701779629  933 PACVKTCPTGAIHFGSKEDMKTLAGERVAELkt-rGYDNAGLYDPagvgGTHVMYVLHHADKPTLYH 998
gi 747132536  170 PACVKTCPTGAIVFGSKEDMLHHAEGRIADLre-rGFARAGLYDPkgveGTHVMYVLHHADKPELYS 235
gi 861642410  158 PACVKTCTTGALAFGERGDMLDLAEVRVQGLke-rGFANAGIYNPqgvgGTHVVYVLPHADKPEIYT 223
gi 659915521  172 PSCVKSCTTGALRFGTREDMLFYSEKRVNELke-rGFAKAGLYNPegvgGTGMLMILHDVTQPETYE 237
gi 116223126  173 PACIKACPTGCLHFGTKEDMTALAETRAAQLrehsGYTKAGVYDPpgvgGTSVIYVLHDATNPEAYG 239
gi 931436507  169 PACVKTCPTNALQYDDRDKLVAEGRKRVEALka-aGWTNANLYGEnelgGLHVMYVLDDHPSVYALP 234
gi 931515142  167 PACVKSCPTDALKYGGRGELSAEGRKRIQALqa-rGYTNAYLYGEkelgGLHVMYVLGDSPEVYGLP 232

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