Conserved Protein Domain Family
NapF_like

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cd10564: NapF_like 
NapF, iron-sulfur subunit of periplasmic nitrate reductase
This family contains NapF protein, the iron-sulfur subunit of periplasmic nitrate reductase. The periplasmic nitrate reductase NapABC of Escherichia coli likely functions during anaerobic growth in low-nitrate environments; napF operon expression is activated by cyclic AMP receptor protein (Crp). NapF is a subfamily of the beta subunit of DMSO reductase (DMSOR) family. DMSOR family members have a large, periplasmic molybdenum-containing alpha subunit as well as a small beta FeS subunit, and may also have a small gamma subunit. The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.
Statistics
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PSSM-Id: 319886
View PSSM: cd10564
Aligned: 120 rows
Threshold Bit Score: 129.286
Threshold Setting Gi: 802397621
Created: 15-Jan-2016
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Fe-S cluster
Feature 1:Fe-S cluster binding site [ion binding site]
Evidence:
  • Comment:predicted based on similar proteins.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #      #### ##   #   #              # #      #  ####  #   ##  ##         
gi 836454970  20 RLPWMVNDDIFTDGCTRCGKCAAACETQIIIh---------GDGGFPEVDFKRGECTFCYRCADVCPEPLFHPQtt---- 86
gi 46915433   49 RPPGAVEEGVFLQLCQSCNYCETACVQQVIE----------MVEGRPQLNLDYNHCTQCGECQKACLSGALSSKn----- 113
gi 767118258  35 RPPTAVDEDIFKRLCTQCGQCRTVCPEHVII----------FKDGYPVINIEYSACTLCFKCKAVCPTNALSNL------ 98
gi 404627308  27 RPPWAIEESQYLTLCTRCGECFKACPRGLLKpatleeyegsPIAGTPVLDLACGQCSYCGSCARACPTGALDLQlg---- 102
gi 197314995  50 RSPYAIEEGLFRELCQDCEQCAAVCPQHVIE----------MVDARPQLNLDYNHCTFCGECQLVCDSGALGKDl----- 114
gi 944716662  47 RPPQAVAESLFIQRCSGCGNCTQACPVGMLH----------LQEGKPALSIEYADCDFCGACTQACETGALDASvr---- 112
gi 684609005  59 RPPKALDETLFLQICDGCGTCATSCPEQVIE----------VVEGKAQLNLDYGYCSQCGGCNQVCPTGALHGSi----- 123
gi 451932030  35 RPPHAVDEALFVRLCDGCGECLKVCPNSVIE----------LDAQLAQLNLNYNQCSLCSECVKACPSDALHASvp---- 100
gi 377540819  45 LPPGASSATQFLTACTRCGQCADACPMGVIIq---------REDGYPQLAIEFASCDGCGQCIEACTTDVLQHQa----- 110
gi 885131362  17 RPPGAIEEVLFQKICTGCTECELACPETIIH----------VESGLAQLDIDYGYCSMCGECQKACPTGALHAKklqdkq 86
                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
Feature 1              #               ####   ### ##               # #####  #   #    #     
gi 836454970  87 pAWAMVAEIGEG--CLAYqhVECRSCGDMCETQAIRFRLqpggvAQPELEATNCTGCGACVSVCPTSAITVVNP 158
gi 46915433  114 nDIGLRPSFSKG--CTNRlsGECLICVDACPQQAISVSSr----QLPVIDTAVCSGCGQCRSACFIGAVQLEWQ 181
gi 767118258  99 -LNDTGLRVHITntCINEy-VYCNECETSCPVSALQWQDk----QIPTIDHDKCIGCGLCKNDCYIEAITMQEE 166
gi 404627308 103 rQVQTRVQIEES--CQARqgFYCLLCEDACPQQAIKATA-----DGINVNMDACNGCGACGLACLHGAITLIRQ 169
gi 197314995 115 gIINLQPEFISS--CNNKlsGGCEVCADACPQKAIIIEPr----KLPQVDASLCNGCGLCRSACFIGAVQMTFA 182
gi 944716662 113 aDTGLRPVHLPQ--CLGRmdDMCRMCEIACPRTAIFFNQq----NQPSVDDEKCDGCGKCKLVCYHGDIRLSLN 180
gi 684609005 124 sDLAMRPVFSMS--CQNAifGDCYLCKEPCQTNAITITDy----ELPRVDNSKCDGCGRCKQSCPFSAIQIDYV 191
gi 451932030 101 lDINLRPDFTQS--CDNYlqTECQQCASACPQHAISVQDd----ELPHINNELCNGCGQCRISCYSNAILMKLY 168
gi 377540819 111 -HFDTRLRPSFStqCTHAs-RGCRQCATACPVQACAFTEs----ALPVVNNERCNGCGECMIQCDRQAITLAQC 178
gi 885131362  87 nDTELRPSFSTN--CQNRlfGECELCAEQCPHQAISIADf----CLPELDQAKCDGCVRCKQACPFSVITMNLL 154

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