Conserved Protein Domain Family
7tm_TAS2R4

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cd15013: 7tm_TAS2R4 
mammalian taste receptor 2, subtype 4, member of the seven-transmembrane G protein-coupled receptor superfamily
This group includes the mammalian taste receptor 2 (TAS2R) subtype 4, which functions as a bitter taste receptor. The human TAS2R family contains about 25 functional members, which are glycoproteins and have the ability to form both homomeric and heteromeric receptor complexes. Five basic tastes are perceived by animals: bitter, sweet, sour, salty, and umami (the taste of glutamate, MSG). Among these, sour and salty are mediated by ion channels, while the perception of umami and sweet tastes is mediated by the TAS1R taste receptors, which belong to the class C GPCR family. The TAS2Rs in humans have a short extracellular N-terminus and the ligand binds within the transmembrane domain, whereas the TAS1Rs have a large N-terminal extracellular domain composed of the Venus flytrap module that forms the orthosteric (primary) ligand binding site. Signal transduction of bitter taste involves binding of bitter compounds to TAS2Rs linked to the alpha-subunit of gustaducin, a heterotrimeric G protein expressed in taste receptor cells. This G-alpha subunit stimulates phosphodiesterase and decreases cAMP and cGMP levels. Further steps in the signaling cascade is still unknown. The beta-gamma-subunit of gustducin also mediates bitter taste transduction by activating phospholipase C, which leads to an increased formation of IP3 (inositol triphosphate) and DAG (diacylglycerol), thereby causing release of Ca2+ from intracellular stores and enhanced neurotransmitter release.
Statistics
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PSSM-Id: 320141
View PSSM: cd15013
Aligned: 5 rows
Threshold Bit Score: 363.743
Threshold Setting Gi: 89111404
Created: 21-Jan-2014
Updated: 26-Jul-2017
Structure
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Aligned Rows:
  next features
Feature 1:TM helix 1 [structural motif]
Evidence:
  • Comment:All members of this receptor family contain a seven-transmembrane helix domain.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         ##########################                                                     
gi 29839665    7 FSAIIASVILNFVGIIMNLFITVVNCKTWVKshrISSSDRILFSLGITRFLMLGLFLVNTIYFvssnte-rsvylSAFFV 85
gi 29839645    7 VFVFAASVFLNFVGIIANLFIIVIIIKTWVNsrrIASPDRILFSLAITRFLTLGLFLLNSVYIatntg--rsvyfSTFFL 84
gi 81890708    7 AFVFAASVVFNFVGIVANLFIIVIISKTWVKshkISSSDKILFSLAITRFLTLGLFLLNTVYIatntg--rsvyfSTFFL 84
gi 89111404   11 IFFMIVALTQLLTGIVGNLLIMIVNCKAWIKsrkLSSFDRILSSLSITRCISLSLMFLHLIFSlifpkskypelmALIIL 90
gi 617599931   6 SSVLIASAVFNFVGLLANLFIAVVNYKSWMQnhrISSSDRILFSLAITRFLMLGSFLLLVIYQflfplvlrsvfvLIFSR 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 29839665   86 LCFMFLDSSSVWFVTLLNILYCVKITNFqhsVFLLLKRNISPKIPRLLLACVLISAFTTCLYitlsqaspfpelv--ttr 163
gi 29839645   85 LCWKFLDANSLWLVTILNSLYCVKITNFqhpVFLLLKRTISMKTTSLLLACLLISALTTLLYymlsqisrfpehi--igr 162
gi 81890708   85 LCWKFLDSNSLWLVTFLNCLYCVKITHFqhpVFLLLKRTVSMKTTSLLLACLLISAFTTLLYfvltqisrfpehi--igr 162
gi 89111404   91 NSWLFLDSCSLWIVTLLNIFYCVKIANLnhlLFLWIKRNLSLKTPWPLLACLLVPIISNFLQillnkifqlvhptqenvr 170
gi 617599931  86 LCWVFLESTSLWFMTLLNTLYCVKITNFqhsVFLLLKRNLSPKTPWLLLACVLISFFITLLYfvlrltspippym--lgr 163
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 29839665  164 nntsfniseGILSLVVSLVLSSSLQFIINVTSASLLIHSLRRhiqkmqknatGFWNPQTEAHVGAMKLMVYFLILYIPYS 243
gi 29839645  163 ndtsfdlsdGILTLVASLVLNSLLQFMLNVTFASLLIHSLRRhiqkmqrnrtSFWNPQTEAHMGAMRLMICFLVLYIPYS 242
gi 81890708  163 ndtlfdvsdGILTLAASLILSSLLQFLLNVTFASLLIHSLRRhvqkmqrnrsSFWNPQTEAHVGAMRLMICFLVLYIPYS 242
gi 89111404  171 nstmvningSIFLLMAPVILSFSLPFIINLVSCSLLISSLRKhmlemqknatSFWNPQTKAHVGAMKVMIYFLILYVPYS 250
gi 617599931 164 nnteidlnaEVLPILMSVALSCCLQFILNVASTSLVIKSLWQhikmmqknntKFWNPRTEALAGAMKQMIYFLMAYVPHS 243
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*....
Feature 1                                                         
gi 29839665  244 VATLVQylpfyaGMDMGTKSICLIFATLYSPGHSVLIIITHpKLKTTAK 292
gi 29839645  243 IATLLYlps-ymRKNLRAQAICMIITAAYPPGHSVLLIITHhKLKAKAK 290
gi 81890708  243 IAALLYfps-ymRKNLRAQAACMIITAAYPPGHSILLIITHhKLKAKAK 290
gi 89111404  251 IAQLVIflpsfgIRNNWIRSILIIVNCTYSPGHTIFIILLHpKLKARIK 299
gi 617599931 244 VLMLLFylniaiGETVEHLTIYSFFSTLYHPGHSVLIVLTHpRLKAEAK 292

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