Conserved Protein Domain Family
7tm_Opsin-1_euk

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cd15028: 7tm_Opsin-1_euk 
proton pumping rhodopsins in fungi and algae, member of the seven-transmembrane GPCR superfamily
This subgroup represents uncharacterized proton pumping rhodopsins found in fungi and algae. They belong to the microbial rhodopsin family, also known as type I rhodopsins, consisting of the light-driven inward chloride pump halorhodopsin (HR), the outward proton pump bacteriorhodopsin (BR), the light-gated cation channel channelrhodopsin (ChR), the light-sensor activating transmembrane transducer protein sensory rhodopsin II (SRII), and the other light-driven proton pumps such as blue-light absorbing and green-light absorbing proteorhodopsins, among others. Microbial rhodopsins have been found in various single-celled microorganisms from all three domains of life, including halophile archaea, gamma-proteobacteria, cyanobacteria, fungi, and green algae. While microbial (type 1) and animal (type 2) rhodopsins have no sequence similarity with each other, they share a common architecture consisting of seven-transmembrane alpha-helices (TM) connected by extracellular loops and intracellular loops. Both types of rhodopsins consist of opsin and a covalently attached retinal (the aldehyde of vitamin A), a photoreactive chromophore, via a protonated Schiff base linkage to an amino group of lysine in the middle of the seventh transmembrane helix (TM7). Upon the absorption of light, microbial rhodopsins undergo light-induced photoisomerization of all-trans retinal into the 13-cis isomer, whereas the photoisomerization of 11-cis retinal to all-trans isomer occurs in the animal rhodopsins. While animal visual rhodopsins are activated by light to catalyze GDP/GTP exchange in the alpha subunit of the retinal G protein transducin (Gt), microbial rhodopsins do not activate G proteins, but instead can function as light-dependent ion pumps, cation channels, and sensors.
Statistics
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PSSM-Id: 320156
View PSSM: cd15028
Aligned: 22 rows
Threshold Bit Score: 247.195
Threshold Setting Gi: 58397465
Created: 10-May-2005
Updated: 26-Jul-2017
Structure
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Aligned Rows:
  next features
Feature 1:putative ligand binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 32699624   41 ETGHRTLWVTFALMVLSSGIFALLSWNVPTskRLFHVITTLITVVASLSYFAMATghattfncdtawdhh-khvpdtshq 119
gi 46095956   35 QWGHTYLWVVFFIMAGFSLLFFISSQRASYryRLMHTTTFFITAIAALSYFAMATgvgktlstig-----------gghh 103
gi 58397465   53 RVGHVSVWVFTALFIAGLVVALVLTSRTQKknRLFHGISAVILTVSALTYMSLAThigstfvpiygppg----hheplvh 128
gi 154319009  51 QSGQRTLWVVFVIMLLSTLTFFYLAWRVPVqkRLFHVLTAFITLFATLSYYAMAIgdgnalthiilrevh-ehtpdtveh 129
gi 501750000  23 STGYNILWVVFILMLLATASFVVLSLRVKKemRLFHYITAMITLTAAVSYYAMAThsgttfskv-------------gkd 89
gi 440638953  44 HAGHVALWVGFIIMLLSTLAFISMAWRVPVekRVFHIIATFIAAFATISYFGMATgggnsfvhmvasnchkhtdpvqdvd 123
gi 358059197  35 EAGKRTLWITFVVMAVASLIFLLMSFRQPAkaRIFHHVTFFITATAAVSYYCMASgmgnaiikap------------gnh 102
gi 521779928  39 PTGAKTLWVVFLIMLISSITFVALSFRVPVqkRLFYVLTTFMTIFATTFYFVMATgsgfywinsnln--------liqdv 110
gi 392575188  51 YAGHVALWVVFAFFTLGLLGVLALSLRVEKkaRVFHWLSAAVLTIAMVSYLAMATgmgisyvpikysg-----phagelh 125
gi 328851545  34 RLSHSVLWVVFAIMILSTVVIGILTTQQQKnnKLFHHITLTINFIAAISYYSMASgigislsh----------------h 97
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    #  ##                                   #   #                    #  
gi 32699624  120 vcrqvfWGRYVDWALTTPLLLLELCLLAGvd--------gAHTLMAIVADVIMVLCGLFAAlgegg---ntaqKWGWYTI 188
gi 46095956  104 aprefyFARYIDWALTTPLLLFDLTLLAGlp--------vAEIIVLVLADEVMIVTGVIAGvhpt-----ktgKWGFFTF 170
gi 58397465  129 ffrqvfSIRYIDAAITGPLTILALSRLAGvspatalsaalAQLVVVYSAWAASVGGGWPWGkhgk----gagtKWAWFAV 204
gi 154319009 130 vyrqvfWARYVDWSLTTPLLLLDLSFLAGln--------gANILVTIVADLVMVLTGLFAAyhnd-----gaaKWGWYAM 196
gi 501750000  90 hyrevfYARYIDWSITTPLLLLDLCFLAGtp--------gIDIVALIFADLGMILTGLFASlesh-----ktySWGWYTM 156
gi 440638953 124 vfrqlfWARYVDWLLTTPLILLSLALLAGln--------gADILVTLVADAAMILTGLFAAlget-----kgqRWGFYII 190
gi 358059197 103 afrevyYARYLDWLITTPLLLLDCCLLAGvp--------aAEIVLIILADIGMILTGLLAGvddg-----rttRWGLFTI 169
gi 521779928 111 slrqvfWVRFLDLIFTTPFIILDLAFLSNmn--------gASITTALVSDTLMIILSIFATfsts-----qgqKWSYFAF 177
gi 392575188 126 yfrevyWARYVDWLFTTPLLLLSLALLAGls--------pADTLITIGADVFMIVTGLLSTihsshsnagervKWLFYAV 197
gi 328851545  98 garplfWARYLDWLLTTPLLLTDLGLLAGip--------iQDIVLLVLSDVVMVVTGLLGAinqs-----estKWGYFVM 164
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ##  #                                    #  ##  #                     #  ##     
gi 32699624  189 GCFSYLFVIWHVALHGSRtvtakGRGVSRLFTGLAVFALLLWTAYPIIWGIAggarrtnvDTEILIYTVLDLLAKPVFGF 268
gi 46095956  171 SCIAFAWVLLQLVTSGRStaflrSPKVGGLYNQVSLALVVVWTAYPVAFALCegtgklnpDAEILFFAILDVIAKPLWGA 250
gi 58397465  205 ADLAFLAVWTVLLAKGRKasvhrARPTQGLFYLLSSMIILIHIGQGVIWILTdginlisvNAEIISYGIMDVAAKIGFTH 284
gi 154319009 197 GCVAFLVIIYQLVVPGRRavstkDAKTSKLFAAIAGYTLIIWTLYPIVWGIGdgsrtwsvDAEIIAYAVLDVLAKPVFGL 276
gi 501750000 157 ACIFYLYIVYVLVLNGRSsaasrGQKVSNLFDAVALFTLVLWTLYPVAWGLSegagilnvDQEIAFYAVLDVLAKGVFGL 236
gi 440638953 191 AWLAYLVVVYQLVIGGRRnasqrGSGTARFFGAVGGFILVLWTIYPIIWALGagkgklsvDCEIIWYVVLDALAKPVFAL 270
gi 358059197 170 SCLFFLYVLWGLLFSARNtafgkSDRVGKLYLGIAVYTAVLWIAYPVVWAFAegtnsitgDTEVLAYAILDIAAKAVFGG 249
gi 521779928 178 SCLAYLSIVYQFAVPGRRavatkASSTRKLFTAIGSFTCILWTLYLIVWALGdcshtygvNTEIVAFAVLDILTKPVFGF 257
gi 392575188 198 SCVGFVVIWWILLSGGLKaaklrPSKTRGLFHLLGVMTFHLWLAYPIVFALSegankisvNAEIIAYGVLDVAAKLGFTY 277
gi 328851545 165 SCIFFVYVVYELATTARRtsylkSSRVGFLFTAIAVYSMLLWTVYPVVWALSegssiispDLEILFYAVLDVMAKAVFGF 244

                 ....*.
Feature 1              
gi 32699624  269 WLLLSH 274
gi 46095956  251 WLLLAT 256
gi 58397465  285 LLLLLH 290
gi 154319009 277 WLLFAH 282
gi 501750000 237 WLLTGH 242
gi 440638953 271 WLLSTH 276
gi 358059197 250 WLLLAH 255
gi 521779928 258 WLLIAH 263
gi 392575188 278 MLLLVH 283
gi 328851545 245 WLLIAH 250

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