3UG9


Conserved Protein Domain Family
7tm_ChRs

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cd15241: 7tm_ChRs 
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channelrhodopsins, member of the seven-transmembrane GPCR superfamily
Channelrhodopsins (ChRs) are light-gated ion channels acting as sensory photoreceptors in unicellular green algae, controlling phototaxis (directional movement toward or away from light). ChRs are large seven-transmembrane proteins with large C-terminal extensions, which have been implicated in localizing the channel to the algal eyespot, a single layer of pigmented granules, overlaying part of the plasma membrane but are not required for ion channel function. ChRs are belongs to the microbial rhodopsin family, also known as type I rhodopsins, comprising the light-driven inward chloride pump halorhodopsin (HR), the outward proton pump bacteriorhodopsin (BR), the light-sensor activating transmembrane transducer protein sensory rhodopsin II (SRII), the light-sensor activating soluble transducer protein Anabaena sensory rhodopsin (ASR), and the other light-driven proton pumps such as blue-light absorbing and green-light absorbing proteorhodopsins, among others. Microbial rhodopsins have been found in various single-celled microorganisms from all three domains of life, including halophile archaea, gamma-proteobacteria, cyanobacteria, fungi, and green algae. While microbial (type 1) and animal (type 2) rhodopsins have no sequence similarity with each other, they share a common architecture consisting of seven-transmembrane alpha-helices (TM) connected by extracellular loops and intracellular loops. Both types of rhodopsins consist of opsin and a covalently attached retinal (the aldehyde of vitamin A), a photoreactive chromophore, via a protonated Schiff base linkage to an amino group of lysine in the middle of the seventh transmembrane helix (TM7). Upon the absorption of light, microbial rhodopsins undergo light-induced photoisomerization of all-trans retinal into the 13-cis isomer, whereas the photoisomerization of 11-cis retinal to all-trans isomer occurs in the animal rhodopsins. While animal visual rhodopsins are activated by light to catalyze GDP/GTP exchange in the alpha subunit of the retinal G protein transducin (Gt), microbial rhodopsins do not activate G proteins, but instead can function as light-dependent ion pumps, cation channels, and sensors.
Statistics
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PSSM-Id: 320369
View PSSM: cd15241
Aligned: 15 rows
Threshold Bit Score: 385.521
Threshold Setting Gi: 575828307
Created: 16-Feb-2014
Updated: 26-Jul-2017
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:putative ligand binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                               #  #  ## 
3UG9_A        66 LAANILQWITFALSALCLMFYGYQTWKSTCgWEEIYVATIEMIKFIIEYFHEFDEPAVIYssngnktvWLRYAEWLLTCP 145
gi 451913047 105 DAAMVWAYIVFAICIIQLVYFMFAAWKATVgWEEVYVNVIELVHIALVIWIEFDKPAMLYlndgqmvpWLRYSAWLLSCP 184
gi 355340880  97 TFAHVCQWSIFAVCVLSLLWYAYQYWKATCgWEEVYVCCIELVFICFELYHEFDSPCSLYlstsnvvnWLRYSEWLLCCP 176
gi 355340884  88 LGAIVAMFIVFGSCVGALIFYGVAAWRTTCgWEEVYVCVIELAHVLIAIFHEIESPSTLYlstgnqvlWLRYAEWLLSCP 167
gi 575827565  63 VAAFAIAWVVFSLSVLQLLYYAYAQWRSTCgWEEVYVGIIELTHICIAIFREFDSPAMLYlstgnfvvWARYASWLLSCP 142
gi 401709451  94 AASIAFQWLAFATAVIGLMFYAWDTWKATTgWEEVYVCTIELIKVLIEIFKEFEIPCSLYlptgnwvlWLRYAEWLLTCP 173
gi 575827846  50 SLSNACDWFAFAISVIFLVYYAWAAFNSSVgWEEIYVCTVELIKVSIDQFLSSNSPCTLYlstgnrvlWIRYGEWLLTCP 129
gi 575827828  50 LGANVCMWMAFAACLLCLMFYAYSTWRATCgWEEVYVCLVEMVKVMIEVFHENDSPATLYlstgnfimWIRYGEWLLSCP 129
gi 575828339 128 KVLRGFQWVAFAFSLGVLAYYAYNSWKATTgWEEVYVCIVELIKVCLEIWHEPDHPATLYlsagnfilWLRYGEWLLTCP 207
gi 575827602  67 KIAVGLQWFTVIVAIVQLIFYGWHSFKATTgWEEVYVCVIELVKCFIELFHEVDSPATVYqtnggaviWLRYSMWLLTCP 146
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #                           #   #               #  ##  #                       
3UG9_A       146 VILIHLSNLTGLandYNKRTMGLLVSDIGTIVWGTTAALskg-yvRVIFFLMGLCYGIYTFFNAAKVYIEAYhtvPKGRC 224
gi 451913047 185 VILIHLSNLTGLkgdYSKRTMGLLVSDIGTIVFGTSAALsppnhvKVILFIIGLLYGLYTFFTAAKVYIEAYhtvPKGHC 264
gi 355340880 177 VILIHLSNVTGLsddYGRRTMGLLVSDIATIVFGVTAAMlvn-wpKIIFYLIGFTMCCYTFFLAAKVLIESFhqvPKGIC 255
gi 355340884 168 VILIHLSNLTGMkddYSKRTMGLLISDIGTIVFGTTAAMsppnylKVIFWFCGLSYGVTTFYLAAKVYIEAYhtvPKGIC 247
gi 575827565 143 VILIHLSNLTGMkgnYSKRTMALLVSDIGTIVWGSTSAMsphnhvKIIFFFLGLVFGLFTFYAAAKVYLEAYhtvPKGKC 222
gi 401709451 174 VILIHLSNITGLkddYNKRTMRLLVSDIGCIVWGVTSAMtvg-ylKWIFFAIGLLYGSNTYFHSAKVYIEAYhtvPKGRC 252
gi 575827846 130 VILIHLSNVTGLkdnYSKRTMALLVSDIGTIVFGVTSAMctg-ypKVIFFILGCCYGANTFFNAAKVYLEAHhtlPKGSC 208
gi 575827828 130 VILIHLSNITGLqdqYSKRTMQLLVSDLGTITMGVTAALcsn-yvKWIFFIAGLCYGVNTYFHAAKVYIESYhivPKGIC 208
gi 575828339 208 VILIHLSNLTGLkdnYSKRTMMLLISDIGCIVWGVTSALttltwlKWFFFFIGLCYGLSTFFHAGRVYIESYmmlPKGQC 287
gi 575827602 147 VILIHLSNLTGLheeYSKRTMTILVTDIGNIVWGITAAFtkg-plKILFFMIGLFYGVTCFFQIAKVYIESYhtlPKGVC 225
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                      #  ##  #                      #  ##           
3UG9_A       225 RQVVTGMAWLFFVSWGMFPILFILGPEGfgvlsvYGSTVGHTIIDLMSKNCWGLLGHYLR 284
gi 451913047 265 RNLVRAMAWTYFVSWAMFPILFILGREGfghityYGSSIGHYILEIFSKNLWSLLGHGLR 324
gi 355340880 256 RHLVKAMAITYFVGWSFFPLIFLFGQSGfkkispYADVIASSFGDLISKNAFGMLGHFLR 315
gi 355340884 248 RKIVRFMAWDYFGSWCMFPILFVLGPEGfghisaYGSVIAHQVLDITSKNIWSMAGHFLR 307
gi 575827565 223 RNIVRFMAWTYYVTWALFPILFILGPEGfghityYGSSIGHYVLEIFSKNLWSGTGHYLR 282
gi 401709451 253 RVIVRLMAYCFYLAWTMFPILFALGPEGmgqmsaYMSTILTTIADVLSKQIWGLLGHHLR 312
gi 575827846 209 RTLIRLMAYTYYASWGMFPILFVLGPESfghmnmYQSNIAHTVIDLMSKNIWGMLGHFLR 268
gi 575827828 209 RICVRVMAWCFFAAWTCYPLLFVFGPEGlgvlsyNASAIGHTIIDIFSKQVWGFVGHYLR 268
gi 575828339 288 RKILRIMAGVYYFSWSAFPILFILGPEGfghisaYASTIGHTINDMLSKQLWTLLGHHLR 347
gi 575827602 226 RKICKIMAYVFFCSWLMFPVMFIAGHEGlglitpYTSGIGHLILDLISKNTWGFLGHHLR 285

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