Conserved Protein Domain Family
7tmB2_GPR128

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cd15257: 7tmB2_GPR128 
orphan adhesion receptor GPR128, member of the class B2 family of seven-transmembrane G protein-coupled receptors
GPR128 is an orphan receptor of the adhesion family (subclass B2) that belongs to the class B GPCRs. Expression of GPR128 was detected in the mouse intestinal mucosa and is thought to be involved in energy balance, as its knockout mice showed a decrease in body weight gain and an increase in intestinal contraction frequency compared to wild-type controls. The adhesion receptors are characterized by the presence of large N-terminal extracellular domains containing multiple adhesion motifs, which play critical roles in cell-cell adhesion and cell-matrix interactions, that are coupled to a class B seven-transmembrane domain. These include, for example, EGF (epidermal growth factor)-like domains in CD97, Celsr1 (cadherin family member), Celsr2, Celsr3, EMR1 (EGF-module-containing mucin-like hormone receptor-like 1), EMR2, EMR3, and Flamingo; two laminin A G-type repeats and nine cadherin domains in Flamingo and its human orthologs Celsr1, Celsr2 and Celsr3; olfactomedin-like domains in the latrotoxin receptors; and five or four thrombospondin type 1 repeats in BAI1 (brain-specific angiogenesis inhibitor 1), BAI2 and BAI3. Furthermore, almost all adhesion receptors, except GPR123, contain an evolutionarily conserved GPCR- autoproteolysis inducing (GAIN) domain that undergoes autoproteolytic processing at the GPCR proteolysis site (GPS) motif located immediately N-terminal to the first transmembrane region, to generate N- and C-terminal fragments (NTF and CTF), which may serve important biological functions.
Statistics
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PSSM-Id: 320385
View PSSM: cd15257
Aligned: 4 rows
Threshold Bit Score: 341.853
Threshold Setting Gi: 32965075
Created: 11-Nov-2013
Updated: 26-Jul-2017
Structure
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Aligned Rows:
  next features
Feature 1:putative polypeptide ligand binding pocket [polypeptide binding site]
Evidence:
  • Comment:based on mutagenesis of human glucagon receptor (GCGR), and modeling studies of GCGR and other related class B GPCRs
  • Comment:Residues in the globular N-terminal extracellular domain and the extracellular loops of the 7TM domain may also be involved in ligand binding.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #   #                                                 #  ##  #           
gi 229462926 431 PKSLDILSNVGCALSVTGLALTVIFQIVTRKVRKTSv-------TWVLVNLCISMLIFNLLFVFGIENSnknlqtsdgdi 503
gi 483503970 425 AKPLECISYVGVGLSIAGLVITILFQIFTRKTRKSSv-------TWMLVSLCSSMLTFNIIFISGIENQnarnhssntsg 497
gi 32965075  215 DVTLSIISTIGCVTSIVCLVITIILHLHNKKLRSLRp-------TKIFLNVTSNLALFYVIFTMGVSRTdnd-------- 279
gi 410906365 253 AQALDWISTIGLCLSIAGLVVTIVHHLEYNFCKFSGdskgsvkaRVVLLCIYITLLAFIITFLSGVRNYsrqyeistvpd 332
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                     #      #                                           
gi 229462926 504 nnidfd------nndiprtdtinipnpmCTAIAALLHYFLLVTFTWNALSAAQLYYLLIrtmkplprHFILFISLIGWGV 577
gi 483503970 498 seqqystyyttnntllksdlvdppadswCMAVAVLLHYFLLATFMWTALNCAQLYLLLIramkplpgNFIVTMSAIGWGI 577
gi 32965075  280 --------------------------dvCTAVAMTMHYVILVTWMWMVVYSFNIYTAVAmmf--skeMTILQAAIFAYLL 331
gi 410906365 333 retn---------tvllseeyvepdtgpCTAVTALLHFFLLASFLWNCVYGTQLVLLLRslhsslppYWTKLSHAVGWGI 403
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                        ## # #          
gi 229462926 578 PAIVVAITVGVIysqngnnpq----------------weldyrqekICWLaipep--ngviKSPLLWSFIVPVTIILISN 639
gi 483503970 578 PAVVVAVTLGATyregkal---------------------nyrqeeFCWLaaldqdqnfsiKKPMLWSFLLPVALILLFN 636
gi 32965075  332 PVVIVGATSGASiafptslpvdedrfcdsspptiyenftrtyrsqdKCWIh----------EGALLYGFLLPVGVCYVTC 401
gi 410906365 404 PAVVTAITLAITyraddpl---------------------gyrqeeFCWLaaldkakrfdyRKPMFWGFLLPVGLILIYN 462
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                    #  #               #   #            
gi 229462926 640 VVMFITISIKVLWKNNqnltstkkvSSMKKIVSTLSVAVVFGITWILAYLMLVNdd-siRIVFSYIFCLFNTTQGLQIFI 718
gi 483503970 637 IIIFIKIIVSVIWKENkdltrnkkdSFMGKITSTISVVVVLGITWTTGYFMLINqe-etSLVFSYVFCILNATQGIQICI 715
gi 32965075  402 VILFIMTMQKILKKQVkkk----krSYHRERYIAVTMATMFGVTWVFGYVILVVndplsKSVTSWLFAVTSSIQGVMIFI 477
gi 410906365 463 IVLLVLTSMTTCKTNKnlqs-tkhlSLRKKVLISFSVAVLLGLSWILGYLVLVTsg-qsHLVFSIIFCICTTTQGFQIFI 540
                        330
                 ....*....|....*....
Feature 1                           
gi 229462926 719 LYTVRTKVFQSEASKVLML 737
gi 483503970 716 LYTFRSPIFKKKVSKMSSA 734
gi 32965075  478 LMGWRKPEFRQLFPSLSLK 496
gi 410906365 541 LFTARTPSFRKNVSLSLQR 559

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