Conserved Protein Domain Family
7tmB1_NPR_B4_insect-like

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cd15260: 7tmB1_NPR_B4_insect-like 
insect neuropeptide receptor subgroup B4 and related proteins, member of the class B family of seven-transmembrane G protein-coupled receptors
This subgroup includes a neuropeptide receptor found in Nilaparvata lugens (brown planthopper) and its closely related proteins from mollusks and annelid worms. They belong to the B1 subfamily of class B GPCRs, also referred to as secretin-like receptor family, which includes receptors for polypeptide hormones of 27-141 amino-acid residues such as secretin, glucagon, glucagon-like peptide (GLP), calcitonin gene-related peptide, parathyroid hormone (PTH), and corticotropin-releasing factor. These receptors contain the large N-terminal extracellular domain (ECD), which plays a critical role in hormone recognition by binding to the C-terminal portion of the peptide. On the other hand, the N-terminal segment of the hormone induces receptor activation by interacting with the receptor transmembrane domains and connecting extracellular loops, triggering intracellular signaling pathways. All members of the B1 subfamily preferentially couple to G proteins of G(s) family, which positively stimulate adenylate cyclase, leading to increased intracellular cAMP formation and calcium influx. The class B GPCRs have been identified in all the vertebrates, from fishes to mammals, as well as invertebrates including Caenorhabditis elegans and Drosophila melanogaster, but are not present in plants, fungi, or prokaryotes.
Statistics
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PSSM-Id: 320388
View PSSM: cd15260
Aligned: 18 rows
Threshold Bit Score: 339.634
Threshold Setting Gi: 556093828
Created: 11-Nov-2013
Updated: 26-Jul-2017
Structure
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Aligned Rows:
  next features
Feature 1:putative polypeptide ligand binding pocket [polypeptide binding site]
Evidence:
  • Comment:based on mutagenesis of human glucagon receptor (GCGR), and modeling studies of GCGR and other related class B GPCRs
  • Comment:Residues in the globular N-terminal extracellular domain and the extracellular loops of the 7TM domain may also be involved in ligand binding.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #   #                                         #  ##  #              #    
gi 549137507 114 REFVNSLYVTGYSVSSAALLISLLIFLTFRSlRCTRIAIHVHLFVSFAANNLMWIVWYKQVvgvtkvvqendiYCQALHI 193
gi 270008144  89 RTHINHLFVIGYSISLAALVISLAIFFTFRTlKCTRIRIHIQLFISFALNNLMWIIWYKEVvpnpfvtirnelWCQALHL 168
gi 161077789 191 RQFINELYVKGYALSLLALLISIIIFLGFKSlRCTRIRIHVHLFASLACTCVAWILWYRLVversetiaenplWCIGLHL 270
gi 167873341 164 RRLVNDLYIGGYTISLVTLIVSLCVFFSFRTlKCTRIRIHINLFISLALSCIFWLVWYKLVveqpevthrsgnWCISLHI 243
gi 357612140 122 RSTVNEIYVGGYSISVAALILSLFIFFYFKSlHCTRIRVHMNLFTAFALNNILWIVWYRVVvnnatvvqenkiWCQLLHI 201
gi 546683807  99 YKFINFLYSVGYTISSIALLVSLVIFFSFRSlRCTRIRIHVQLFISFLFYNILAIIWYAEVvikvdvtngnpvWCLFLYI 178
gi 195947048 124 RNVINNMYVGGYAISLIALILSLMIFVFLRHfQYKRTRIHANLFISFILNNIMWIVWYKTVvdyievvqentiWCQSLHI 203
gi 443686399 103 DKRVINVFIAGYTVSILFLIASLLIFTFFKQlQCDRVTIHKHLFTSYIFNGAVWILYYTTAamdaqvqannpvWCQAVHV 182
gi 556093828 119 DYGYIYVFIAGYSLSLLLLVISLAIFFVFRQlRCERITIHKNLFFSYLFTGLTWILYYSLVilnghvilqnpiWCQALHV 198
gi 405957368  68 NFAHVYVSVVGFCVSLILLICSLVIFFKFRQlRCDRITVHKNLFVSYIITAFCFVIYLSVVsfgdgvlmenpvWCQVLHV 147
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          #                                                                       ## # #
gi 549137507 194 ILQYLMVANYMWMFCEGLHLHLALVVVFvkddCAMRLFYFIGWCLPLFITTVYTLVRmsyphdtsqCWMg-DSYSQWVLI 272
gi 270008144 169 VVHYLMLANYMWMFCEGLHLHLALVVVFvrdaETMKWFFALGWGAPFIIVLIYSVVRifilkdnymCWMadSYYSSWILT 248
gi 161077789 271 VVHYFMLVNYFWMFCEGLHLHLVLVVVFvkdtIVMRWFIVISWFSPIPIAIVYGLARhfsspdnkhCWIt-DSLYLWIFS 349
gi 167873341 244 LLHYLMLVNYFWMFCEGLHLHLVLVIVFikdaIAMRWFMTIGWIVPMGLISFYATFRnnytadtehCWMd-ESHAMWLLT 322
gi 357612140 202 VTTYFMVTGYTWMFCEGLHLHIALVVVFvkekVAMRCFLAIGWGLSLVIIIVYAVVRyhipgaterCWMe-QSEAFWIIT 280
gi 546683807 179 AREYFMVANYIWMFCEALHLHIALVVVFvaeeKIMKWFYGFGWGVPSVIVLVHNLVRvyysgdvgrCFMeeVTFSTWFII 258
gi 195947048 204 LTYYHMMTSYMWMFCEGLHLHVGLVSINe--yISVRSYCAIGWAIPALVVALYTGVRmqlrygterCWMd-QSHALWIIV 280
gi 443686399 183 ITQYFVTCNFFWMFCEGLYLHTIIVVAFstgkKLLICCYVLGWVLPIFITALYTAARalspdeakaCWLh-ESTLQWIVA 261
gi 556093828 199 LAQYFTVCNFAWMFCEGVYLHTIMVQAYrsgkTLLICCIVFGWSVPLLLTAIYTGVRgslelhstdCWIf-ADKYQWILF 277
gi 405957368 148 ITQYAVVSNFSWMFCEGLCLHTLMMHTFisgnSLIIVCLIIGWCVPLVLTGIYAGLRatltdntkfCWLs-ESSLQWIMF 226
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                   #  #               # 
gi 549137507 273 VPVLLSMLASLAFLINVVRVLLTKLHcnsan-----papiGMRKAVRAALILIPLFGIHHILIPFRPEPha-plEMTYQI 346
gi 270008144 249 APVCISLLVSLIFLINVLRVILTIMHpnsan-----papmGLRRAARAALILIPLFGLQHILIPFRPDMyd-pyEHLYQY 322
gi 161077789 350 VPITLSLLASFIFLINVLRVIVRKLHpqsaq-----paplAIRKAVRATIILVPLFGLQHFLLPYRPDAgt-qlDHFYQM 423
gi 167873341 323 VPVFLSLAASLVFLVNVVRVLLTKLNstspn-----paplGLKKATRATLILVASRFLLLLAPLVTSRCaa--nETYRRV 395
gi 357612140 281 VPVVISLIASFVFLINVVRVLLTKLHpaphr-----yssvATKKAARAALILIPLFGLHFVLIPFRPSPdm-pgEKLYQV 354
gi 546683807 259 VPITITLAISMGFLINILRVILTIMHpsspn-----papdSVRKAVRAALILTPLFGLQFIFIPVKPDAqh-plYTVHQY 332
gi 195947048 281 IAVVIILTLSFLFLVNVIRVLLKKMQppasnqpneaakkaARATATLVIYFMIPLYGLHFILIPFQRAPes-igEKIYQV 359
gi 443686399 262 GPIVLTIVINFIFLCNIVRVLVTKLRsvns------pdtnGYRKAVRATLILIPLLGLQFILFPLRPEEnt-twDEIYML 334
gi 556093828 278 GPVVASVAINICLLINIIRLLVTKLRqip--------easQSKKAARATLILVPLFGLQYLIMPVKPGKds-pfYDFYHY 348
gi 405957368 227 APVVFSMLINIIFVINIMRLLVTKLKqmp--------eaaQTRKAVRATGILIPLLGLQFLVFIKRPNDkdstlYDVYTY 298
                        250       260       270
                 ....*....|....*....|....*....|....*..
Feature 1          #                                  
gi 549137507 347 FSALLVSLQGFCVSVLFCFANv---DVHCAFKNVLRR 380
gi 270008144 323 VTVVVVTLQGLCVSCLFCFANq---DVHQAIRGFMHR 356
gi 161077789 424 LSVVLVSLQGFVVSFLFCFANh---DVTFAIRTLLNK 457
gi 167873341 396 GRPCGKSCGTYGQLCAMKRINrcqcLVGFARNRSGAC 432
gi 357612140 355 VSALLTSLQGVCVAILFCFTNh---DVVAAAKTYISR 388
gi 546683807 333 ISMVIGSYQGLCCAILYCFANh---EVHQAIKRSFQR 366
gi 195947048 360 VSALLTSLQGLCVSILFCFTNn---DVKTALTNYLAR 393
gi 443686399 335 VSAVVTSCQGLFVAVIFCFLNg---EVHTVIKRRWNQ 368
gi 556093828 349 FIALLTSLQNYDPTSGFSPTVc--vKTVFFWILDLKL 383
gi 405957368 299 AVAIVVSLQGVGSGAPPCDSTl---PAILSLDPRMVR 332

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