Conserved Protein Domain Family
7tmB1_NPR_B7_insect-like

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cd15273: 7tmB1_NPR_B7_insect-like 
insect neuropeptide receptor subgroup B7 and related proteins, member of the class B family of seven-transmembrane G protein-coupled receptors
This subgroup includes a neuropeptide receptor found in Nilaparvata lugens (brown planthopper) and its closely related proteins from invertebrates. They belong to the B1 subfamily of class B GPCRs, also referred to as secretin-like receptor family, which includes receptors for polypeptide hormones of 27-141 amino-acid residues such as secretin, glucagon, glucagon-like peptide (GLP), calcitonin gene-related peptide, parathyroid hormone (PTH), and corticotropin-releasing factor. These receptors contain the large N-terminal extracellular domain (ECD), which plays a critical role in hormone recognition by binding to the C-terminal portion of the peptide. On the other hand, the N-terminal segment of the hormone induces receptor activation by interacting with the receptor transmembrane domains and connecting extracellular loops, triggering intracellular signaling pathways. All members of the B1 subfamily preferentially couple to G proteins of G(s) family, which positively stimulate adenylate cyclase, leading to increased intracellular cAMP formation and calcium influx. The class B GPCRs have been identified in all the vertebrates, from fishes to mammals, as well as invertebrates including Caenorhabditis elegans and Drosophila melanogaster, but are not present in plants, fungi, or prokaryotes.
Statistics
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PSSM-Id: 320401
View PSSM: cd15273
Aligned: 8 rows
Threshold Bit Score: 396.356
Threshold Setting Gi: 270006438
Created: 11-Nov-2013
Updated: 26-Jul-2017
Structure
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Aligned Rows:
  next features
Feature 1:putative polypeptide ligand binding pocket [polypeptide binding site]
Evidence:
  • Comment:based on mutagenesis of human glucagon receptor (GCGR), and modeling studies of GCGR and other related class B GPCRs
  • Comment:Residues in the globular N-terminal extracellular domain and the extracellular loops of the 7TM domain may also be involved in ligand binding.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #   #                                         #  ##  #                   
gi 549137513 119 VPIVKFVSQVGYSVSLFTLIIAFCIFATFKGLRCPRNKLHMHLFLSFMLRAFSTLFKDLETnrlerfsatsg-------- 190
gi 546685497  36 VDLIMGISETGYAISLITLLIAFTIMFKIKKLHCARNILHMNLFASFILRSFMHIMKNSLFmdglglskdiverngsnvf 115
gi 316974041 165 LRIAQLIGVIGYCASLVSLSLASAVFLHFKRLHCSRNMVHLNLFLAFMLRAFMCLLQYSLFrngkttlfdgdltid---- 240
gi 270006438 115 LPIIKNITQCGYILSTVSLIISLFVFIRIKRLHCARNKLHIHLFASFVMRALMSLIKDGLFiegtalpheiiqin----- 189
gi 212510071 121 LPILKGISHIGYGISLISLVMASLILLSFRKLYCSRNTLHLHLFVSFIMRAFVTFLKEFLFeqgiglrsnfveqn----- 195
gi 321477804 110 LPIIRRISQVGYGVSLGTLLIALCILASFKKLYCPRNTLHMHLFISFIMRAFMALLRDNLFvdgldslalstdealv--- 186
gi 307174829 143 LPIIRIVSQIGYSTSFITLIVAMVIFSLLRKLRNPRNRLHMHLFVSFIMRAFMALLKDWIFidgigmawnvvfvd----- 217
gi 270016404 135 APVVQVISETGYIVSFATLIIAFAIMLFIKKLHCARNILHMHLFASFILRALTFIVIKSTFveglglpsdlnyrng---- 210
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                             #      #                                                   
gi 549137513 191 ---------elislredwasCRAVVTLWQYCLLANYSWILMEGLYLHNLIFlalf-sdtSSISLYVALGWGLPLLFVAIW 260
gi 546685497 116 hvqadvgvftsianignnfeCKLIVSLVQYFTTANYSWILMEGLYLNNLILralftdsnKNLIYYITFGWGLPLLVVVPW 195
gi 316974041 241 -----gngnekfadngshwvCKSIITMWNYSLIASYFWQLMEGMYLHNFVYifv--fheTRVILYVLLGWGLPMIIMVPW 313
gi 270006438 190 -------gklvynktnfswvCKAIISLWNYFIISNYMFLLMEGAYLHNLLFlkl--lseNGVVIYYSLGWGIPLLFIIPW 260
gi 212510071 196 ------gtkyfldeinsnweCKSLTTIWQYFIMANYSWILMEGLYMHNLLMfalf-sdnANIFMYIILGWGLPIIFVISW 268
gi 321477804 187 ----eaasdgvlhqypgtsgCKLVTSLWQYFIMANYSWILMEGLYLHNLIFlalf-tdsSAITIYIILGWGLPFIFVSSW 261
gi 307174829 218 -------gnnafinehsmgiCKAITSLWQYFIVANYSWILMEGLYLHNLVFkalc-adtNTIALYILLGWGLPILVVVPW 289
gi 270016404 211 ------slyfdinsetnnwaCKLLTSLWQYFITANYSWILMEGLYLHNLIFralfadssNSIKWYVVMGWGLPLIIVGFW 284
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                             ## # #                                                     
gi 549137513 261 ALARivweNSMCWTtn-eNALIVMLVKGPTTISVLLNFVLFIKIVRVLLTKLQasvn--eqnrRFKRWAKSTLVLMPLFG 337
gi 546685497 196 VIARilfdDTLCWTtn-dNFEVFLIIIIPTFTSVLVNLVLFVIISVVLYNKLKspin--edsrRYLKWAKSTLVLVPLFG 272
gi 316974041 314 IVVRlkdeNSLCWVqy-sNLNNFWILRGPIMLTIVINFILCMNICRELFIKLRqshl--tdlrRYKKFAKSTFLLIILLG 390
gi 270006438 261 IVLKagneNIYCWTtk-sSKFIAMLIDVPIGLTVVINFILFTIIVRILFVKLTsmyiq-qrwtKYQKLIRAILILVPLFG 338
gi 212510071 269 ALVRinleNDLCWTtn-lISRNFLIIRIPIIISVLVNFGLFLNIIRLLLLKVKsavq--eetqKYWRLAKSTIVLAPLFG 345
gi 321477804 262 VVMRiifeNLLCWTtnenKIIFWLLIRGPITASILVNLIFFLHIAWVLLSKLRssvsaetrkyRYRKWAKSTLVLVPLFG 341
gi 307174829 290 IIIRatieDTLCWTth-kNPLLFLIIRIPIIISILFNFLLFLNIVRVLLVKLKtsvhlqrkkmKYKRWAKSTMILVPLFG 368
gi 270016404 285 VAARllveDNLCWTth-eNYDVFLIIGIPTMVSILINLLLFMRISMVLYSKLRspin--edsrRYQKWVKSTLVLVPLFG 361
                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1          #  #                   #   #                               
gi 549137513 338 VHYAIFISMSYVdcg---peiEIIWLFGDQLFASFQGSFVALLYCFMNgEVRAELNKKCLR 395
gi 546685497 273 VNYAIFLIFYFLn-------eQTIWMVGDALFGSFQGFFVAILYCFLNgEVKAEIKPYMST 326
gi 316974041 391 GYYFVMSIPMLImdtakltaqLLACLFVEHVLSSLQGVLVATVYCFCNsEVRAEIQRHWQR 451
gi 270006438 339 IPYTISFVLSFYaled--qtfEIMWLFFDQTFTAFQGLFASLVYCLLNsEVQMEIMRKYNS 397
gi 212510071 346 VHYTIFLGMSYCvgin--etiELIWLFCDQLFASFQGFFVALFFCFLNnEVQTEMKKKLRQ 404
gi 321477804 342 VHYTVYCIMHGAdglg--eavEVALLVSDQLFASFQGFLVALLYCLMNgEVRGELSRRWKR 400
gi 307174829 369 IHYSFFLGLSYHkdy----rvELIWLFCDQLFASFQGALVALLYCLLNgEVRAEMRRAWRA 425
gi 270016404 362 VHYALFLALYYLiktn--kivEVVWLFCDLLFGSFQGFFVAILYCFLNgEVKSEIQPHLYY 420

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