2G9B


Conserved Protein Domain Family
EFh_HEF_CB

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cd16176: EFh_HEF_CB 
Click on image for an interactive view with Cn3D
EF-hand, calcium binding motif, found in calbindin (CB)
CB, also termed calbindin D28, or D-28K, or avian-type vitamin D-dependent calcium-binding protein, is a unique intracellular calcium binding protein that functions as both a calcium sensor and buffer in eukaryotic cells, which undergoes a conformational change upon calcium binding and protects cells against insults of high intracellular calcium concentration. CB is highly expressed in brain and sensory neurons. It plays essential roles in neural functioning, altering synaptic interactions in the hippocampus, modulating calcium channel activity, calcium transients, and intrinsic neuronal firing activity. It prevents a neuronal death, as well as maintains and controls calcium homeostasis. CB also modulates the activity of proteins participating in the development of neurodegenerative disorders such as Alzheimer's disease, Huntington's disease, and bipolar disorder. Moreover, CB interacts with Ran-binding protein M, a protein known to involve in microtubule function. It also interacts with alkaline phosphatase and myo-inositol monophosphatase, as well as caspase 3, an enzyme that plays an important role in the regulation of apoptosis. CB contains six EF-hand motifs in a single globular domain, where EF-hands 1, 3, 4, 5 bind four calcium ions with high affinity.
Statistics
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PSSM-Id: 320076
View PSSM: cd16176
Aligned: 5 rows
Threshold Bit Score: 436.962
Threshold Setting Gi: 632969970
Created: 18-Nov-2014
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:Ca binding site [ion binding site]
Evidence:
  • Comment:The consensus sequence of the EF-hand calcium-binding loop is D#x#D/N#G#D/S/N#G#x#I/L/V#x#x#x#E/D, where the uppercase letters represent residues that occur with more than 50% frequency and #x# stands in positions with less defined preference.
  • Comment:Due to the lack of key residues involved in Ca2+ coordination, the EF-hand motifs 2 and 6 are non-canonical, low-affinity Ca2+-binding motifs.
  • Structure:2G9B; Rattus norvegicus Calbindin D28k binds four Ca2+ ions through its EF-hand motifs 1, 3, 4, and 5.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                # # #      #                                                            
2G9B_A        18 FFEIWLHFDADGSGYLEGKELQNLIQELLqarkkaglelspEMKTFVDQYGQRDDGKIGIVELAHVLPTEEnflllfrcq 97
gi 115393     17 FFEIWHHYDSDGNGYMDGKELQNFIQELQqarkkagldltpEMKAFVDQYGKATDGKIGIVELAQVLPTEEnfllffrcq 96
gi 213626987  16 FLEIWRHYDTDGNGFIEGKELQDFILELQqarkkagldlseQMKAFVDQYGQSSDGKIGIAELAQILPTEEnfllffr-q 94
gi 642116368  16 FLDIFHHYDNDGNGYIEGKELQSFIKELQqarkqagleltdQMKAFVQEYEKNTDAKIGIVELVQILPTEEnfllffr-q 94
gi 632969970  19 FLEIWHHYDNAGRGYIEGRELQSFIRELQqarkatgselsdGMKALVEKYDQSADGKIEMADLAQILPTEEnfflffr-q 97
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                       # # #      #                                # # #      #         
2G9B_A        98 qlksceEFMKTWRKYDTDHSGFIETEELKNFLKDLLekanktvddtklaeYTDLMLKLFDSNNDGKLELTEMARLLPVQE 177
gi 115393     97 qlksseDFMQTWRKYDSDHSGFIDSEELKSFLKDLLqkankqiedsklteYTEIMLRMFDANNDGKLELTELARLLPVQE 176
gi 213626987  95 qlksseEFMQSWRRYDTDHSGFIETDELKSFLKDLLkkankpceeskleeYTHTMLRMFDTNNDGKLGLTELSMLLPVQE 174
gi 642116368  95 qlkscaEFMQAWRCYDADHSGYIEADELKNFLKDLLqkakkpydenkldeYTHTTLKIFDSNHDGKLCLAEMARLLPDEE 174
gi 632969970  98 qlkssaEFMETWRIFDADHSGFIEADELKSFLRDLLkkanrpfdevkleeYTQTTLKMFDSNNDGKLGLAEMARLLPDKE 177
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                               # # #      #                                             
2G9B_A       178 nfllkfqgikmcgkEFNKAFELYDQDGNGYIDENELDALLKDLCeknkqeldinnISTYKKNIMALSDGGKLYRTDLALI 257
gi 115393    177 nflikfqgvkmcakEFNKAFEMYDQDGNGYIDENELDALLKDLCeknkkeldinnLATYKKSIMALSDGGKLYRAELALI 256
gi 213626987 175 nfllkfqgvkmcgkEFNKVFELYDKDGNGHMDENELDDLLKDLCeknkkdldinsLSTYKKSIMALSDGGKLYRAELALV 254
gi 642116368 175 nfllkfqnvkmvrrEFNKIFELYDQDENGYMDENELDALLKDLCeknkkvlevnkIPTYKTAIMALSDGGKLYRTELALV 254
gi 632969970 178 nfllrfqgvnmsskEFDDIFEFFDQDGNGYIDENELDALLKHLCeknkedldiqnVSMYKKSIMALSDGGKLFRKDLALI 257

                 ....
Feature 1            
2G9B_A       258 LSAG 261
gi 115393    257 LCAE 260
gi 213626987 255 LCAD 258
gi 642116368 255 LCAE 258
gi 632969970 258 LCAE 261

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