Conserved Protein Domain Family
EFh_HEF_CR

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cd16177: EFh_HEF_CR 
EF-hand, calcium binding motif, found in calretinin (CR)
CR, also termed 29 kDa calbindin, is a cytosolic hexa-EF-hand calcium-binding protein predominantly expressed in a variety of normal and tumorigenic t specific neurons of the central and peripheral nervous system. It possibly functions as a calcium buffer, calcium sensor, and apoptosis regulator, which may be implicated in many biological processes, including cell proliferation, differentiation, and cell death. CR contains six EF-hand motifs within two independent domains, CR I-II and CR III-VI. CR I-II consists of EF-hand motifs 1 and 2, and CR III-VI consists of EF-hand motifs 3-6. The first 5 EF-hand motifs are capable of binding calcium ions, while the EF-hand 6 is inactive. Thus, CR has two pairs of cooperative binding sites (I-II and III-IV), which display high affinity calcium-binding sites, and one independent calcium ion-binding site (V), which displays lower affinity binding.
Statistics
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PSSM-Id: 320077
View PSSM: cd16177
Aligned: 5 rows
Threshold Bit Score: 447.784
Threshold Setting Gi: 632988702
Created: 18-Nov-2014
Updated: 18-Aug-2016
Structure
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Aligned Rows:
  next features
Feature 1:Ca binding site [ion binding site]
Evidence:
  • Comment:The consensus sequence of the EF-hand calcium-binding loop is D#x#D/N#G#D/S/N#G#x#I/L/V#x#x#x#E/D, where the uppercase letters represent residues that occur with more than 50% frequency and #x# stands in positions with less defined preference.
  • Comment:Calretinin binds five Ca2+ ions through its EF-hand motifs 1, 2, 3, 4, and 5. Due to the lack of key residues involved in Ca2+ coordination, the EF-hand motif 6 is a non-canonical, low-affinity Ca2+-binding motif.
  • Citation:PMID 9368033

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                # # #      #                                   # # #      #             
gi 109940061  21 FLEIWKHFDADGNGYIEGKELENFFQELEkarkgsgmmsksdnfgeKMKEFMQKYDKNSDGKIEMAELAQILPTEEnfll 100
gi 115644     19 FLDVWRHFDADGNGYIEGKELENFFQELEsarkgtgvdskrdslgdKMKEFMHKYDKNADGKIEMAELAQILPTEEnfll 98
gi 41152295   21 FLDIWKHFDADGNGYIEGKELDNFFSQLEiarrgagvdpsnaafkgRMTEFMQKFDKNKDGRIEMSELAQILPTEEnfll 100
gi 632988702  20 FLEIWKHYDSDGNGYIEGKELENFIRELQrvrrg-------akveiHVKEFMEKYDKNADGRIEMAELAQILPTDEnfll 92
gi 556999230  20 FLEIWKHFDSDGNGYIEGKELENFFRELEnarrgagvettsvnfaeKMKEFMKKYDKNADGRIELSELVQILPTDEnfll 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                           # # #      #                                # # #      #     
gi 109940061 101 cfrqhvgssaEFMEAWRKYDTDRSGYIEANELKGFLSDLLkkanrpydepklqeYTQTILRMFDLNGDGKLGLSE-MSRL 179
gi 115644     99 cfrqhvgsssEFMEAWRRYDTDRSGYIEANELKGFLSDLLkkanrpydeaklqeYTQTILRMFDMNGDGKLGLSE-MSRL 177
gi 41152295  101 cfrqfvgsstEFMAAWRKYDTDRSGFIEANELKGFLSDLLkkanrhyddqklqeYTQTILKMFDLNGDGKLGLSE-MARL 179
gi 632988702  93 cfrqcvhssgEFMETWRKYDTDRSGYIEANELKSFLLDLLqkanrhyddkklqeYTQTILRMFDANGDGKLGLSE-MARL 171
gi 556999230 100 cfrqyagsssEFMEAWRRYDTDRSGYIEANELKGFLSDLLkkaqrpyeesklleYTQTIVKGDNLNGDGWTFPLTsVVRL 179
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                    # # #      #                                        
gi 109940061 180 LPVQEnfllkfqgmkltseEFNAIFTFYDKDRSGYIDEHELDALLKDLYeknkkemniqqLTNYRKSVMSLAEAGKLYRK 259
gi 115644    178 LPVQEnfllkfqgmklsseEFNAIFAFYDKDGSGFIDEHELDALLKDLYeknkkemsiqqLTNYRRSIMNLSDGGKLYRK 257
gi 41152295  180 LPVQEnfllkfqgvrlsaeQFNAIFTYYDKDGNGYIDEQELDALLKDLYeknnkevdsksLTGYKQSIMALSDGGKLYRS 259
gi 632988702 172 LPVQEnfllkfqgmklsteEFNAIFAFYDKDGNGYIDKNELDALLKDLYekixkemdvqkFSAYKKNILSLSDGGRLYRR 251
gi 556999230 180 LPVQEnfllkfqgmkltsdEFNAIFAFYDRDGSGYIDDNELDALLKDLYeknrkemniqnLTSYKKSIMSLSDGGKLYRK 259

                 ....*....
Feature 1                 
gi 109940061 260 DLEIVLCSE 268
gi 115644    258 ELEVVLCSE 266
gi 41152295  260 ELEIVLCRD 268
gi 632988702 252 ELAMVLCTE 260
gi 556999230 260 ELEMVICQD 268

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