Conserved Protein Domain Family
EFh_PEF_peflin

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cd16184: EFh_PEF_peflin 
EF-hand, calcium binding motif, found in peflin and similar proteins
Peflin, also termed penta-EF hand (PEF) protein with a long N-terminal hydrophobic domain, or penta-EF hand domain-containing protein 1, is a ubiquitously expressed 30-kD PEF protein containing five EF-hand motifs in its C-terminal domain and a longer N-terminal hydrophobic domain (NHB domain) than any other member of the PEF family. The NHB domain harbors nine repeats of a nonapeptide (A/PPGGPYGGP). Peflin may modulate the function of ALG-2 in Ca2+ signaling. It exists only as a heterodimer with ALG-2, and binds two Ca2+ ions through its EF1 and EF3 hands. Its additional EF5 hand is unpaired and does not bind Ca2+ ion but mediates the heterodimerization with ALG-2. The dissociation of heterodimer occurs in the presence of Ca2+. In lower vertebrates, peflin may interact with transient receptor potential N (TRPN1), suggesting a potential role of peflin in fast transducer channel adaptation.
Statistics
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PSSM-Id: 320059
View PSSM: cd16184
Aligned: 22 rows
Threshold Bit Score: 264.897
Threshold Setting Gi: 332376755
Created: 18-Nov-2014
Updated: 18-Aug-2016
Structure
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Aligned Rows:
  next features
Feature 1:putative Ca binding site [ion binding site]
Evidence:
  • Comment:Peflin may harbor two Ca2+-binding sites through its highly conserved EF1 and EF3 hands.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 # # #      #                                                       # # 
gi 74761895  118 EAYSWFQSVDSDHSGYISMKELKQALVNCNwssfndeTCLMMINMFDKTKSGRIDVYGFSALWKFIQQWKNLFQQYDRDR 197
gi 19921976   34 QAQQWFSMVDRDRSGKINASELQAALVNGRgdhfsdnACKLMISMFDNDASGTIDIYEFEKLYNYINQWLQVFKTYDQDS 113
gi 675879226 104 NVFQWFQAVDRDRTGKINANELQMALTNANwskfnldTCRLMITMFDRDKSGQIDINEFQGLWTYIHQWKTIFDQFDRDR 183
gi 675893020  52 QVHQWFLSVDHDKSGKINMHELQQALTNANwthfnaeTCRLMIGMFDKDQSGQIEMNEFQALWNYIIQWKSVFEQFDRDR 131
gi 242018867  33 EIQQWFNAVDVDRSGKITAKELQSALVNGQgknfnlpVCELLISMFSKDQSGTVNVDEFQHLYKFVNQWLQTFKSFDKDQ 112
gi 443685714  29 VIQQWFVSVDQDRSGQISTSELQQALTNSNwsrfneeTCHLMIGLFDRDMSGTINLGEFQALWTYIQQWKGVFDQFDRDR 108
gi 390364554   8 DVYSWFVAVDQDNTGKIDSRELQQALTNANwskfdeaTCKQMIGMFDRDKSGTIDASEFSQLWNYIQQWKQVFEGFDRDR 87
gi 225709504  83 QIQTWFRAVDTDNSGQIDAAELKKALVNGNwsnfseeACTLMISMFDRTRSGTISINEFGDLYNYINQWKAIFEGIDRDR 162
gi 321461377  30 DLIKWFQAVDQDKSGKISSNELRQALVVGNrshfsieACELLVKMFSSENSRMIDVQGFKQLFHYVNQWKTSFHMFDRDH 109
gi 307174834  20 NINQWFSMVDKDGSGQITAKELQSALANGQggtfsdiACKLMIGMFDKEKNGTINVSEFQALYNYINAWLGVFRGFDHDN 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #      #                                                                        
gi 74761895  198 SGSISYTELQQALSQMGYnlspqFTQLLVSRYCPrsANPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFVT 277
gi 19921976  114 SGHIEEQELTQAFTQMGFrfspeFINFLVKKSDPq-GHKEVSVDQFIVLCVQVQRFTEAFRQRDTQQNGTITIGFEDFLT 192
gi 675879226 184 SGTIDGNELHSAYLQMGYrlspqFSQSVVYRFDMs-GQQRISLDYFIHSCILIKNITEQFRARDTANSGFVQIGYEDFVS 262
gi 675893020 132 SGFIDANELCNALNTMGYkvtpqFANHVVFRYDPq-AKQRLGLDNFIQVCVLIKSVTDMFRQRDSQMKGTIQVAYEDFMT 210
gi 242018867 113 SGVIEEEEVSQAFQQMGYrfsneFIKFLIGRADKv-AKKRISVDQFILVCIQIQRFTDAFRVRDTEMKGVITVSFEDFLT 191
gi 443685714 109 SGFIDANELNNAYTQMGYrlspaFSSMVVFRYDPq-FRRQLSLDNFIQSCVLLKTITDTFRQKDAQAQGVINVGYEEFLS 187
gi 390364554  88 SGGIDANELNTALNQMGYhlspaFSQMVVQKYDTv-HHKQIGLDHYIKLCAVLNSLTASFRQRDTQMTGSININYEDFMM 166
gi 225709504 163 SGFIEQNELMAAFQQMGYrftptFVQNLLSKYDP--QNRRLTLDNFIVSSVQIKRLTDSFRVRDKEMKGQVTLGYEDFVG 240
gi 321461377 110 SGAIDEKELGQALVQMGYrlsdkSVTALLNKFTS--KPGQITFDNFILACVQLHQLTDAFRRHDTQHTGTITIAYEDFIQ 187
gi 307174834 100 SGSIQESELNAALTQMGYrlsseFIEFLIKKSDLr-GHQSITVDQFIVLCVQIQRFTEAFRTRDTDQTGTISINFEDFLG 178

                 ....*.
Feature 1              
gi 74761895  278 MTASRM 283
gi 19921976  193 VAIGCS 198
gi 675879226 263 AVVLNK 268
gi 675893020 211 MVMLNK 216
gi 242018867 192 VALSCS 197
gi 443685714 188 MVMLNR 193
gi 390364554 167 VVLSSQ 172
gi 225709504 241 LALGTH 246
gi 321461377 188 AVVESI 193
gi 307174834 179 VALSCS 184

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