1Y1X


Conserved Protein Domain Family
EFh_PEF_ALG-2_like

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cd16185: EFh_PEF_ALG-2_like 
Click on image for an interactive view with Cn3D
EF-hand, calcium binding motif, found in homologs of mammalian apoptosis-linked gene 2 protein (ALG-2)
The family includes some homologs of mammalian apoptosis-linked gene 2 protein (ALG-2) mainly found in lower eukaryotes, such as a parasitic protist Leishmarua major and a cellular slime mold Dictyostelium discoideum. These homologs contains five EF-hand motifs. Due to the presence of unfavorable residues at the Ca2+-coordinating positions, their non-canonical EF4 and EF5 hands may not bind Ca2+. Two Dictyostelium PEF proteins are the prototypes of this family. They may bind to cytoskeletal proteins and/or signal-transducing proteins localized to detergent-resistant membranes named lipid rafts, and occur as monomers or weak homo- or heterodimers like ALG-2. They can serve as a mediator for Ca2+ signaling-related Dictyostehum programmed cell death (PCD).
Statistics
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PSSM-Id: 320060
View PSSM: cd16185
Aligned: 24 rows
Threshold Bit Score: 204.37
Threshold Setting Gi: 159470455
Created: 19-Feb-2015
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:Ca binding site [ion binding site]
Evidence:
  • Structure:1Y1X; Leishmania major Friedlin PDCD6-like protein binds two Ca2+ ions through its EF1 and EF2 hands.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 # # #      #                             # # #      #                  
1Y1X_A        28 ELMEWFRAVDTDGSGAISVPELNAALSSAGvpf-----slATTEKLLHMYDKNHSGEITFDEFKDLHHFILSMREGFRKR 102
gi 75020351   37 EMSAWFRSIDKDRSGSISAMELQHLHVGYGpl------giETATKLIRVFDVDKSGQIDFYEYAALHQFINILYANFLAN 110
gi 290981337 330 QLKAWFDAVDLDKSGQVSAEELSTVLSKGGmvf-----dkSVTQKMIKMFDRDNSGQIGFHEFEQLHYYLMNMKNAFEKT 404
gi 290992711  10 ALRPLFNAVDLDKSGKITHNELQKVLTMPGselqggsfseRCAKRLVKMFDRNGNGSVDFEEYSALHQYLIQMKAGFESV 89
gi 290993530   5 PLRPFFDAVDLDRSGKITWIELQKALTQPGseftgkvfseRCAKRLIKMFDRNNNAEIDFEEFMQLHQYLLQMKQGFEFV 84
gi 470381459  66 EIQAWFRSVDRDGSGSITANEIAGITFNGVpl------glDVATKLVRVFDRDGNRSIDFYEYAAMHKFLASLQAAFFAA 139
gi 470409524 103 ELQRWFRSMDRDNSGSISANELANVAVGGSvl------giDNGMKLIRVFDVDGNGTIDFNEYASLHKFLLSMQQTFKMG 176
gi 686624440  39 DLMGFFRAVDQSGSGTISVPELNAALSAAGmkf-----syATTERLLSMYDRDFDGEVNFGEFQELHAFIQDMRSGFQSR 113
gi 75020350   45 EMQSWFMRVDANRSGTISSGELQYLNIGGTpl------giETATKLIKVFDHNKNGQIDFYEYAALHQFINNLYRCFVAN 118
gi 407851644  48 EAAQWFRAVSSSSGGYISVPQLQSALSQGGmnf-----syATTERLISMFDANLDGAIDFTEFQGLHRYILSMRGGFSQR 122
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1Y1X_A       103 DsSGDGRLDSNEVRAALLSSGYqvs-eqTFQALMRKFDRqRRGSLGFDDYVELSIFVCRVRNVFAFYDrERTGQVTFTFD 181
gi 75020351  111 DrNRSGTIDAQEIHRALGTSGFnlp-fnTVNLLFLKASP-RGYGLKFSDFLGLCASIAIARSLFEAHDrMRTGVAHLNVN 188
gi 290981337 405 DtNRSGTLDLNEVKTAIQYSGVqvs-psALDRLFKNFDKdKQGTFSFAEYIDFTIFIGICRNSFQLFDkQSTGQATFSFD 483
gi 290992711  90 DtNKSGKVEFNEVTITLSRVGFnfs-pqIVQKLFKLFDFqNKGSLDFDGYIELCAFLGLMRAQFIPRDaNYSGQAMFTWE 168
gi 290993530  85 DtDKSGSLSFDEISRALAQSGYris-piVLQKIFQTVDTqKKGSLNFDGYIELCVYVGIVRNIFQPKDfYRNGQATFTFD 163
gi 470381459 140 DrDRSGTIDAREIHNALAAAGFqvs-lpVVQTFMMVHNK-TGYGVNFHQFLLICATIAQGRSLFQWRDpQRSGRITVTLD 217
gi 470409524 177 DkDGNGRLDAREIHEAILKGGFtnvsfkAAQGLYNKYNR-SGMGLNMAEFISIVAHIALARSVLERLDyDRDGRITVNID 255
gi 686624440 114 DtNGDGRLNGNEVRVAFRSNGYqld-dpTFQALMHHFDRtKQGGLAFDDYIELSIFITKANNTFRTRA-QGNGLATWDFN 191
gi 75020350  119 DrNFSGTIDANEIYNALITSGFqlp-fpTVNYLFLKLSP-SGYGLLFTQFLNLCATVALTRSLFEWNDpMRTGVVHLNLA 196
gi 407851644 123 DtSRDGLLEENEVRMALSANFYqvd-dtTFQTLMRKFDRrKRGSLGFDDYIELSLFVGKANDAFQAQS-QGKAGATFSFS 200

                 ....*....
Feature 1                 
1Y1X_A       182 TFIGGSVSI 190
gi 75020351  189 HIYDIISLV 197
gi 290981337 484 QFLEAAAYI 492
gi 290992711 169 QFIQACMEA 177
gi 290993530 164 QFLEACTEL 172
gi 470381459 218 QLLELVAQM 226
gi 470409524 256 QMYEISANF 264
gi 686624440 192 TFVKAGVSL 200
gi 75020350  197 QLYDIIALV 205
gi 407851644 201 SFLSAGASL 209

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