Conserved Protein Domain Family
EFh_PEF_CalpC

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cd16197: EFh_PEF_CalpC 
Penta-EF hand, calcium binding motifs, found in Drosophila melanogaster calpain-C (CalpC) and similar proteins
CalpC, also termed calcium-activated neutral proteinase homolog C (CANP C), is a catalytically inactive homolog of CalpA and CalpB found in Drosophila. It is strongly expressed in the salivary glands. In contrast with CalpA and CalpB, both of which are closely related to that of vertebrate calpains, and they share similar domain architecture, which consists of four domains: the N-terminal domain I, the catalytic domain II carrying the three active site residues, Cys, His and Asn, the Ca2+-regulated phospholipid-binding domain III, and penta-EF-hand Ca2+-binding domain IV. CalpC is a truncated calpain form missing domain I and about 20 residues from domain II. Moreover, due to all three mutated active site residues (Cys to Arg, His to Val and Asn to Ser), it may not have proteolytic activity.
Statistics
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PSSM-Id: 320072
View PSSM: cd16197
Aligned: 5 rows
Threshold Bit Score: 333.307
Threshold Setting Gi: 229541100
Created: 19-Feb-2015
Updated: 18-Aug-2016
Structure
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Aligned Rows:
  next features
Feature 1:putative Ca binding site [ion binding site]
Evidence:
  • Comment:Based on the structure evidence that Rattus norvegicus m-calpain (3DF0) binds four Ca2+ ions through its EF1, EF2, EF3, and EF4 hands.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    # #    #               #              # # #      #                  
gi 229541100 515 QYEPVYMQLADENKTINCFELHELLEACLpNDYIKGCANidICRQVIALQDRSGSGRITFQQFKTFMVNLKSWQGVFKMY 594
gi 321475083 517 QYEAVFLQLADEHRTVNAFELHELLEACLpNDYIKSCANldVCRQLVLVFDVSGLGRLKLTDFRDFMASLKQWQTAFKSH 596
gi 607360199 501 QYEAVFLQLADEHRTVNAFELQELLEACLpNDYIKSCACmeVCRQVVLTMDSSGSGRLKFNDFKDLMCSLKYWQAAFKNH 580
gi 568256841 515 QYEPVFMQLADDQRTLNCFDLQELLEACLpNDYIRSCASidVCRQVVCLMDKSNRGRINFNDFNKFMINLKTWWGVFKMH 594
gi 391330163 488 TYEQVFLQMADEHKCVNAFDLQEILETCLpNDYVKSCATmeVCRQVVLAMDNTGLGRLRFQDYKQFICSLKWWQTVFKAY 567
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        # # #      #                               # ##                                 
gi 229541100 595 TKEKAGILRAERLRDALCDIGFqlstdIMNCLIQRYIRKDGTLRLSDFVSAVIHLTTAFNQFHLKNYGQVNVIEVHLHDW 674
gi 321475083 597 TKEKTGILRAERFRDALHDVGFqvnndVLSALILRHMRKDGTLRFGDFVSAVLHLTVAFGTFDKRDPLQNGSVKLTLTEW 676
gi 607360199 581 TKEKTGILKAERLRDALLEVGFqlntdVLSILILRYMRKDGTLRFGDFVSAILHLSDAFGIFESKDPLQNGTIKLSLSEW 660
gi 568256841 595 TKEKSGILKAERLRDALFDVGFqlstdNISILILRYMRRDGTLRLADFISAILHLTMAFELFKAKDTNQDGIISMGMTEW 674
gi 391330163 568 TKGTVGVLRADRLKESLEDIGFklnadVLSLLVLRYMRKDGTLRFGDFVACVLHLAAAFGSFEKKDPLQNGFVKLSLQEW 647

                 ....*.
Feature 1              
gi 229541100 675 IKSILS 680
gi 321475083 677 LKYALM 682
gi 607360199 661 LRSSLT 666
gi 568256841 675 IKSVFM 680
gi 391330163 648 LRACLP 653

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