4QJ3,4GNK,2FJU,3QR0


Conserved Protein Domain Family
EFh_PI-PLCbeta

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cd16200: EFh_PI-PLCbeta 
Click on image for an interactive view with Cn3D
EF-hand motif found in metazoan phosphoinositide-specific phospholipase C (PI-PLC)-beta isozymes
PI-PLC-beta isozymes represent a class of metazoan PI-PLCs that hydrolyze the membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2) to propagate diverse intracellular responses that underlie the physiological action of many hormones, neurotransmitters, and growth factors (EC 3.1.4.11). They have been implicated in numerous processes relevant to central nervous system (CNS), including chemotaxis, cardiovascular function, neuronal signaling, and opioid sensitivity. Like other PI-PLC isozymes, PI-PLC-beta isozymes contain a core set of domains, including an N-terminal pleckstrin homology (PH) domain, four atypical EF-hand motifs, a PLC catalytic core, and a single C2 domain. Besides, they have a unique C-terminal coiled-coil (CT) domain necessary for homodimerization. The PLC catalytic core domain is a TIM barrel with two highly conserved regions (X and Y) split by a highly degenerate linker sequence. There are four PI-PLC-beta isozymes (1-4). PI-PLC-beta1 and PI-PLC-beta3 are expressed in a wide range of tissues and cell types, whereas PI-PLC-beta2 and PI-PLC-beta4 have been found only in hematopoietic and neuronal tissues, respectively. All PI-PLC-beta isozymes are activated by the heterotrimeric G protein alpha subunits of the Gq class through their C2 domain and long C-terminal extension. They are GTPase-activating proteins (GAPs) for these G alpha(q) proteins. PI-PLC-beta2 and PI-PLC-beta3 can also be activated by beta-gamma subunits of the G alpha(i/o) family of heterotrimeric G proteins and the small GTPases such as Rac and Cdc42. This family also includes two invertebrate homologs of PI-PLC-beta, PLC21 from cephalopod retina and No receptor potential A protein (NorpA) from Drosophila melanogaster.
Statistics
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PSSM-Id: 320030
View PSSM: cd16200
Aligned: 21 rows
Threshold Bit Score: 146.622
Threshold Setting Gi: 685826019
Created: 17-Nov-2014
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 30 residues -Click on image for an interactive view with Cn3D
Feature 1:EF-hand motif [structural motif]
Evidence:
  • None

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         ##########   ####################                                               
4QJ3_B        155 RKAYTKLKLQ---VNQDGRIPVKNILKMFSADKkr--vETALESCGLk----fNRSESIRPDEFSLEIFERFlnkl--cl 223
gi 699241972  171 EKAYTRLKLE---TNQFGEIPVKKLIKVLGGKIkr--vNHALASVGLvp---gRTDVSIKPDDFHLSGFKKLlqlc--cl 240
gi 541043301  147 QKLFAPIRYAsckELITIDDIVKIIVPCESNISdstriHKALMASELf-----FGKNGIPRTSFTDEHIFDIymai--td 219
gi 4521177    152 YKQYVLSKSM---VNASGKISMKNIFKCLNAVRf---eQTTIEGIQTal---sLPVGEIDPEMFSFEQFYKLcclv--cp 220
gi 4521171    153 KRNYQRILLS---CNGYGQIPVKSICRALLLRVrdktdMELFGIFDAqkkkmdDGTEYIQDTDFTWDKYEQLvnqltkgr 229
gi 340375710  152 RKHYVRMCLE---SNGHGQLPVKVVRKLMMVPKtskdiLNYFESAKTnvkekdDGTPYIDVSEFTEEIYMNLidtllqnr 228
gi 734555970  146 QKLLAPLRYAnckESITVDDIVKAIVPCEGNTTdsahvHKALIADELf-----FKKKYMLRSELTDERIFEIymai--td 218
gi 734546027  167 QRLFTKISHSsgeDFITVNDIVDALIPSKNKEDrr-evERLLKLIPTf-----KDKKSIPSDAITDDFIFTCyrti--td 238
gi 389565924   74 RKMHKRILLS---SKDDKNISIKEIIKMTAKDEkk-rvQEALKAAGIka--ddRKNDLFKKDKFTFEKFHKFykeml-ny 146
gi 699253629  140 RKQWKRIYLP---INNKGLIPVRRLVKSFTSDRnekvvLQKLKTCGLi----yGKKDEISQSEFSFEFYQKLynkm--cs 210
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
4QJ3_B        224 rpDIDKILLEIga-----kGKPYLTLEQLMDFINQKQRDPRLNEVLYPplrpsQARLLIEKYepnqqFLERDQMSMEGFS 298
gi 699241972  241 rpELDSVFSKLgt----gaRRMYVSHQKFKDFINNEQRDPKLNDLLYPfhtddQAQDAITNFepnemFATKGQLTLEGLD 316
gi 541043301  220 rrEIYHIFARRtrdrktriLPGRMELKKVKRFLNHVQRDPRQNEVIFPeecnqSVKKIIDKF-----NGNRKFLTEKAFA 294
gi 4521177    221 rpDIDELFISL--------FGTRLTAEKLMKFLNEDQRDPRLNEQMHPhctisNAVDLIMDYesneaCRTKELLTIQGFI 292
gi 4521171    230 tsDMECVIKDLgk----skKHQQLTLQEFHNFLTKCQRDPRLNTILYPpptkqQAKELAEKYe---gKAVEDRLTPRALL 302
gi 340375710  229 gpDLDVLKKECki----kaRKQYIDAKEFATVLNTQQRDPRLNDILHPrltneQAMALMDKY-----GGEKDRLTMKDLL 299
gi 734555970  219 rrDIRHVFARRtrdrrtrtLPGKMELKKVRRFINHVQRDPRQNELIFPeesleSIKKTVYKF-----AGNRKFLTEKGFA 293
gi 734546027  239 rtDVQSVFNERf------qNERRVTSDLFAKYLKKEHYDPRLNELLYPspnedSAQMLIDEF-----DKTDCCLNVDAFH 307
gi 389565924  147 lpGVSKVFDEIkg----dnRRNYLTIDQMVDFLNKFQRDPRLNEILFPhhnkeGTKKLIQSYeidksKQKIGQLTLDGFT 222
gi 699253629  211 rgDVENIFHEIs-------HNDIIDIDALVEWMNDNQRDPRHNEILVPafdrkQAANIIQKFepsqrNIKNNCLSLEGFT 283

                  ....*..
Feature 1                
4QJ3_B        299 RYLGGEE 305
gi 699241972  317 AYLQSDD 323
gi 541043301  295 KYLLSDE 301
gi 4521177    293 QLLLSKE 299
gi 4521171    303 NFLMSED 309
gi 340375710  300 QYLMSDD 306
gi 734555970  294 KYLLSNE 300
gi 734546027  308 RFLMSSY 314
gi 389565924  223 NFLMSPD 229
gi 699253629  284 LYLASDE 290

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