Conserved Protein Domain Family
EFh_PI-PLCzeta

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cd16204: EFh_PI-PLCzeta 
EF-hand motif found in phosphoinositide phospholipase C zeta 1 (PI-PLC-zeta1)
PI-PLC-zeta1, also termed 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1, or phospholipase C-zeta-1 (PLC-zeta-1), or testis-development protein NYD-SP27, is only found in the testis. The sperm-specific PI-PLC plays a fundamental role in vertebrate fertilization by initiating intracellular calcium oscillations that trigger the embryo development. However, the mechanism of its activation still remains unclear. PI-PLC-zeta1 contains an N-terminal four atypical EF-hand motifs, a PLC catalytic core domain, and a C-terminal C2 domain. The PLC catalytic core domain is a TIM barrel with two highly conserved regions (X and Y) split by a highly degenerate linker sequence. Unlike other PI-PLCs, PI-PLC-zeta is responsible for Ca2+ oscillations in fertilized oocytes and exhibits a high sensitivity to Ca2+ mediated through its EF-hand domain. There is only one PLC-zeta isozyme. Aside from PI-PLC-zeta identified in mammals, its eukaryotic homologs have been classified with this family.
Statistics
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PSSM-Id: 320034
View PSSM: cd16204
Aligned: 4 rows
Threshold Bit Score: 256.275
Threshold Setting Gi: 74714209
Created: 18-Nov-2014
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Feature 1:EF-hand motif [structural motif]
Evidence:
  • None

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ################################                                                
gi 74714209    1 MEMRWFLSKIQDDFRGGKINLEKTQRLLEKLDircsyiHVKQIFKDN-DRLKQGRITIEEFRAIYRIIThreEIIEIFNT 79
gi 637299388   1 MENKWFMDIIFDEFRDGKINLDKTLKLFNKMEisydyvHVKKVFKKN-DSQKKGLITMEDFRAIYRTILhrpELSELFNT 79
gi 86129446    2 EENRWFLNIIQDGFMNGKIDFDSTVKLLEKLHmpfnlaHVKHVFKKTvDKRKIHTINIEDFRAIYRAIVhrnEFHEIFCA 81
gi 565321889   1 FSFTWFLSIIHGRFRKGKINLESTLELLNNFDipfdyiHVKYIFKTS-DSFKAGNITIEDFRRIYRALAhrcEIHELFTI 79
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
Feature 1                                                                       
gi 74714209   80 YSENRKILLASNLAQFLTQEQyaaemskaIAFEIIQKYEPIEEVRKAHQMSLEGFTRYMDSRE 142
gi 637299388  80 YAQNKKVLSAANFVDFLKKEQfevkaderRAGSLISKYEPIEEARKRKEMTFEGFIRYMLSEE 142
gi 86129446   82 YSENRKNLADTELTAFLKKEQfktegaetTALEVILKYEPIDEVRKRRQLSFEGFIRYMSSED 144
gi 565321889  80 HSPNHRILSLPNLVGFLRNEQfqdnadetIASQLVAKFEPIEEAKRNKEMTFEGFIRYVTSED 142

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