Conserved Protein Domain Family
EFh_ScPlc1p_like

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cd16207: EFh_ScPlc1p_like 
EF-hand motif found in Saccharomyces cerevisiae phospholipase C-1 (ScPlc1p) and similar proteins
This family represents a group of putative phosphoinositide-specific phospholipase C (PI-PLC, EC 3.1.4.11) encoded by PLC1 genes from yeasts, which are homologs of the delta isoforms of mammalian PI-PLC in terms of overall sequence similarity and domain organization. Mammalian PI-PLC is a signaling enzyme that hydrolyzes the membrane phospholipids phosphatidylinositol-4,5-bisphosphate (PIP2) to generate two important second messengers in eukaryotic signal transduction cascades, inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol (DAG). InsP3 triggers inflow of calcium from intracellular stores, while DAG, together with calcium, activates protein kinase C, which then phosphorylates other molecules, leading to altered cellular activity. Calcium is required for the catalysis. The prototype of this family is protein Plc1p (also termed 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1) encoded by PLC1 genes from Saccharomyces cerevisiae. ScPlc1p contains both highly conserved X- and Y- regions of PLC catalytic core domain, as well as a presumptive EF-hand like calcium binding motif. Experiments show that ScPlc1p displays calcium dependent catalytic properties with high similarity to those of the mammalian PLCs, and plays multiple roles in modulating the membrane/protein interactions in filamentation control. CaPlc1p encoded by CAPLC1 from the closely related yeast Candida albicans, an orthologue of S. cerevisiae Plc1p, is also included in this group. Like SCPlc1p, CaPlc1p has conserved presumptive catalytic domain, shows PLC activity when expressed in E. coli, and is involved in multiple cellular processes. There are two other gene copies of CAPLC1 in C. albicans, CAPLC2 (also named as PIPLC) and CAPLC3. Experiments show CaPlc1p is the only enzyme in C. albicans which functions as PLC. The biological functions of CAPLC2 and CAPLC3 gene products must be clearly different from CaPlc1p, but their exact roles remain unclear. Moreover, CAPLC2 and CAPLC3 gene products are more similar to extracellular bacterial PI-PLC than to the eukaryotic PI-PLC, and they are not included in this subfamily.
Statistics
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PSSM-Id: 320037
View PSSM: cd16207
Aligned: 41 rows
Threshold Bit Score: 142.388
Threshold Setting Gi: 695113945
Created: 13-Nov-2014
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Feature 1:EF-hand motif [structural motif]
Evidence:
  • None

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #########           ##################                                          
gi 417490     233 ARIHWQITVseke----edeKKDTLSFADVKKLCDKFHiyvstgQLLEFFQLADi-----------nHNGLLNYFEFEKF 297
gi 453089905  377 MAIAWKQHMalk-----dsaDEERLDLKDAMSMCRKLEinctesAVKTHFKAISr-----------dEHMSLDYNQYKDF 440
gi 667830296  362 VRALWTSVMdrkfnrlergdEDERLDFEGVKKICRNLHifcaekIIREQFNRADd-----------dLLNSLNFTQFLRF 430
gi 546234925  310 AKLVWQRAMkkr------ggGDESLDFPSTVELCRSLHincteaTLRAYFSKADt-----------lDAGKLNQQQFLYF 372
gi 300539673  390 VHEFWQRQMrkkyadrvipaEGETIELPDIERICRSLHmhlsptEIRDNFNAAKaakatsngvcdptSPSSLNFNEFKEF 469
gi 629636564  356 VRAHWERETarrsmgnlpkvDEDSLDFQAIESLSRSLHihcskaMLHKQFGLADs-----------rQCGRLNYSEFKDF 424
gi 627801813  246 MSLLWRQAMqlk-----aqdAEEAFTLEDARRFCKQLQinctsrTVESHFHKADa-----------dRKGALSYAQYRTF 309
gi 662527280  242 MRMAWKQATsrk-----npdAEPKIDFEDAKWICRKLEincsinTVRAHFNHADh-----------dLVGMLNYSQYQYF 305
gi 656909562  317 IRAHWDNEIekrsh-rpdqaKVDGLDLSAIRSLCRKLHihapekELELQFNTADa-----------sNSNMLNYEGFRDF 384
gi 759226456  364 IKGIWKRETrg--------dPNARLDFQRAKQLCRKLDvncsehTLKYYFNHADt-----------dNSRDLDYAQFESF 424
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 417490     298 IKILKnrkEVNMIWSKFtkpphSHLSFENFFQFLITEQheqv----drqTAWSYFIKYr----------------ePTQL 357
gi 453089905  441 IQSFRerkDITHIFKNWqlgtdHDMDLETFFDFMRQEQkvdvd--kdraYWQSVFDKFarpaqnrp--slpdavpsSLQK 516
gi 667830296  431 LRRIKkrqDVKKVYESIkkpdePGLNLESFIAFLRDIQgvnvq--sdmnYWVSVFERCcksskski--kvettdveAASL 506
gi 546234925  373 VRRLKerkDIKAIYKTYtsgskIEMDKFTFFTFLRQDQgvdvn--edleYWTNTFEKFaratrprmaatpteggdiPLPL 450
gi 300539673  470 VRLLKarsEISHVFKNHttnpkAGMTRQEFIQFLKEIQcedve--edstHWNNIFSQFarrrrskd---aeqtisgDESL 544
gi 629636564  425 VRRLKqrdDMKSIYSALtedsgSVLTKDQFLSFLQNVQgidts--tdpaKWTSEFNHWvrttnskq--cnhsaqssPGLD 500
gi 627801813  310 VQSFKdrkDIERLHKNLkhgleSDLELDEFLDFLRIDQmvdvd--hdraCWEGIFDRYarpaqtrp--vlpdaepsPYSK 385
gi 662527280  306 VNLFKirkDVQSLYYGIkrsdePELSLEAFLEFLRKEQridve--kdraSWESKFELYcrsasg-------kaadrQLPP 376
gi 656909562  385 VRRLRerrDIKKIFNDLkddsiPGITRSQVVWFLENVQgisfntvseasIWSDKLDDLishsfl-------ddptmRGSG 457
gi 759226456  425 VKSVLerqDLKKIFNSIrqpgnPELDERSFLDFLQHTQgidts--arpeYWANVFETHakrcrpkg--atspvaseHKEY 500
                         170
                  ....*....|....*
Feature 1                        
gi 417490     358 TMGQDGFTKFLKEQP 372
gi 453089905  517 VWTIQSFQSFLTSSY 531
gi 667830296  507 RMSSAAFQAYLASPA 521
gi 546234925  451 AMNFPAFQTYLTSAA 465
gi 300539673  545 TISEAGFTGFLNSHF 559
gi 629636564  501 IMTFEAFSSFMVSPA 515
gi 627801813  386 TMSAQGLQNFLTSSH 400
gi 662527280  377 TMNLQAFQTFLSSGS 391
gi 656909562  458 LVDLNAFASFIMSKD 472
gi 759226456  501 TMNFAAFQDLMTRTS 515

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