Conserved Protein Domain Family
EFh_PI-PLCbeta1

?
cd16208: EFh_PI-PLCbeta1 
EF-hand motif found in phosphoinositide phospholipase C beta 1 (PI-PLC-beta1)
PI-PLC-beta1, also termed 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1, or PLC-154, or phospholipase C-I (PLC-I), or phospholipase C-beta-1 (PLC-beta1), is expressed at highest levels in specific regions of the brain, as well as in the cardiovascular system. It has two splice variants, PI-PLC-beta1a and PI-PLC-beta1b, both of which are present within the nucleus. Nuclear PI-PLC-beta1 is a key molecule for nuclear inositide signaling, where it plays a role in cell cycle progression, proliferation and differentiation. It also contributes to generate cell-specific Ca2+ signals evoked by G protein-coupled receptor stimulation. PI-PLC-beta1 acts as an effector and a GTPase activating protein (GAP) specifically activated by the heterotrimeric G protein alpha q subunits through their C2 domain and long C-terminal extension. It regulates neuronal activity in the cerebral cortex and hippocampus, and has been implicated for participations in diverse critical functions related to forebrain diseases such as schizophrenia. It may play an important role in maintenance of the status epilepticus, and in osteosarcoma-related signal transduction pathways. PI-PLC-beta1 also functions as a regulator of erythropoiesis in kinamycin F, a potent inducer of gamma-globin production in K562 cells. The G protein activation and the degradation of PI-PLC-beta1 can be regulated by the interaction of alpha-synuclein. As a result, it may reduce cell damage under oxidative stress. Moreover, PI-PLC-beta1 works as a new intermediate in the HIV-1 gp120-triggered phosphatidylcholine-specific phospholipase C (PC-PLC)-driven signal transduction pathway leading to cytoplasmic CCL2 secretion in macrophages. PI-PLC-beta1 contains a core set of domains, including an N-terminal pleckstrin homology (PH) domain, four atypical EF-hand motifs, a PLC catalytic core, and a single C2 domain. Besides, it has a unique C-terminal coiled-coil (CT) domain necessary for homodimerization. The PLC catalytic core domain is a TIM barrel with two highly conserved regions (X and Y) split by a highly degenerate linker sequence.
Statistics
?
PSSM-Id: 320038
View PSSM: cd16208
Aligned: 6 rows
Threshold Bit Score: 274.063
Threshold Setting Gi: 82264549
Created: 18-Nov-2014
Updated: 18-Aug-2016
Structure
?
Aligned Rows:
 
Feature 1:EF-hand motif [structural motif]
Evidence:
  • None

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         ##############################                                                  
gi 12643814   153 EKAYTKLKLQVTPEGRIPLKNIYRLFSADRkrvETALEACSLPSSRNDSIPQEDFTPEVYRVFlnnlcprpEIDNIFSEF 232
gi 676708265   73 EKAYTKLKLQVTPDGRIPLKNIYRLFSADRkrvETALEACNLPSSRNDSIPQDDFTPDVYRVFlnnlcprpEIDHIFSEF 152
gi 66911561   154 QKAYTKLKLQVNPEGRIPVKNIYRLFSADRkrvETALEACNLPAGRSDSVPQDDFTPEVYKVFlnnicprpEIDNIFSEF 233
gi 208342308  154 EKAYTRLKLQLNPEGRIPVKNIFRMFSADRkrvETALENCNLPSGRNDSVPQEDFTPEVYNMFlsnicsrsELDHIFSDV 233
gi 632977549  125 DKVYTKLKLQMNQDARIPVKNIVRMFSSDRkrvETALDSSGLPSVRGDSIPVEDFSPEAFRTFvtnigprpEIDHIFSEF 204
gi 82264549   157 LKAYVRLTLQPNAEGRITVKNIVRLFSSDRkrvENALENCKLPYSRGDSIKLEDFTLEVYRSFldslcprsELSSIFKLQ 236
                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1                                                                                
gi 12643814   233 GaKSKPYLTVDQMMDFINLKQRDPRLNEILYPplkqeQVQVLIEKYEPNNSLARKGQISVDGFMRYLSGEE 303
gi 676708265  153 GaKSKPYLTVDQMMEFINLKQRDPRLNEILYPplkqeQVQQLIEKYEPNSNLAKKGQISVDGFMRYLSGEE 223
gi 66911561   234 GsKSKTHLSVDELMEFINSKQRDPRLNEILYPplkqeQVQQLIEKYEHKSILATRGQISVEGFMNYLSGEE 304
gi 208342308  234 GaKSRPYLSVEQMTEFINSKQRDPRLNEILYPplkpeQVQQLVEKYEPNASLVQKGQISVEGFARYLNGEE 304
gi 632977549  205 GaKSRPYLVVDQMTEFINTKQRDPRLNEILYPplkqeQVQQLIEKYEPNITLAKKGQISVDGLKLYLSGDE 275
gi 82264549   237 G-GDEGAMSVDQMTEFINNKQRDPRLNEILYPplrpaQTHTLMERYQHDKTQLEQGIITLQGFSSYLSSDE 306

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap